Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF111 and GFF112 overlap by 4 nucleotides GFF112 and GFF113 are separated by 234 nucleotides GFF113 and GFF114 are separated by 245 nucleotides
GFF111 - Candidate type III effector Hop protein, at 96,669 to 97,940
GFF111
GFF112 - 2-keto-3-deoxygluconate permease (KDG permease), at 97,937 to 98,890
GFF112
GFF113 - FIG01045153: hypothetical protein, at 99,125 to 99,487
GFF113
GFF114 - HNH endonuclease, at 99,733 to 100,575
GFF114
Position (kb) 97
98
99 Strain fitness (log2 ratio) -2
-1
0
1 at 97.399 kb on + strand, within GFF111 at 97.407 kb on - strand, within GFF111 at 97.410 kb on + strand, within GFF111 at 97.411 kb on - strand, within GFF111 at 97.419 kb on + strand, within GFF111 at 97.456 kb on + strand, within GFF111 at 97.486 kb on - strand, within GFF111 at 97.673 kb on + strand, within GFF111 at 97.705 kb on + strand, within GFF111 at 97.930 kb on - strand at 98.335 kb on + strand, within GFF112 at 98.335 kb on + strand, within GFF112 at 98.335 kb on + strand, within GFF112 at 98.465 kb on - strand, within GFF112 at 98.477 kb on - strand, within GFF112 at 98.477 kb on - strand, within GFF112 at 98.530 kb on - strand, within GFF112 at 98.650 kb on + strand, within GFF112 at 98.651 kb on - strand, within GFF112 at 98.651 kb on - strand, within GFF112 at 98.777 kb on - strand, within GFF112 at 99.213 kb on + strand, within GFF113 at 99.300 kb on + strand, within GFF113 at 99.391 kb on + strand, within GFF113 at 99.533 kb on + strand at 99.534 kb on - strand at 99.540 kb on - strand at 99.544 kb on - strand at 99.553 kb on - strand at 99.553 kb on - strand at 99.553 kb on - strand at 99.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 97,399 + GFF111 0.57 -0.0 97,407 - GFF111 0.58 -2.2 97,410 + GFF111 0.58 +0.4 97,411 - GFF111 0.58 +0.5 97,419 + GFF111 0.59 +0.7 97,456 + GFF111 0.62 +0.7 97,486 - GFF111 0.64 -0.1 97,673 + GFF111 0.79 -0.3 97,705 + GFF111 0.81 +0.8 97,930 - +0.7 98,335 + GFF112 0.42 -1.4 98,335 + GFF112 0.42 -0.7 98,335 + GFF112 0.42 +0.9 98,465 - GFF112 0.55 +0.6 98,477 - GFF112 0.57 +1.4 98,477 - GFF112 0.57 -0.3 98,530 - GFF112 0.62 +0.7 98,650 + GFF112 0.75 -0.2 98,651 - GFF112 0.75 +0.2 98,651 - GFF112 0.75 +0.0 98,777 - GFF112 0.88 +0.9 99,213 + GFF113 0.24 -0.4 99,300 + GFF113 0.48 -0.8 99,391 + GFF113 0.73 +0.4 99,533 + -0.2 99,534 - +0.3 99,540 - +1.2 99,544 - +0.3 99,553 - -0.6 99,553 - +0.7 99,553 - -0.1 99,693 - -0.5
Or see this region's nucleotide sequence