Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1043 and GFF1044 are separated by 123 nucleotides GFF1044 and GFF1045 are separated by 51 nucleotides
GFF1043 - Deoxyribose-phosphate aldolase (EC 4.1.2.4), at 224,783 to 225,562
GFF1043
GFF1044 - Thymidine phosphorylase (EC 2.4.2.4), at 225,686 to 227,008
GFF1044
GFF1045 - Phosphopentomutase (EC 5.4.2.7), at 227,060 to 228,283
GFF1045
Position (kb) 225
226
227
228 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 224.695 kb on + strand at 224.695 kb on + strand at 224.696 kb on - strand at 224.696 kb on - strand at 224.924 kb on - strand, within GFF1043 at 225.004 kb on + strand, within GFF1043 at 225.005 kb on - strand, within GFF1043 at 225.157 kb on + strand, within GFF1043 at 225.184 kb on - strand, within GFF1043 at 225.184 kb on - strand, within GFF1043 at 225.400 kb on - strand, within GFF1043 at 225.560 kb on + strand at 225.561 kb on - strand at 225.616 kb on + strand at 225.653 kb on - strand at 225.712 kb on + strand at 225.781 kb on + strand at 225.781 kb on + strand at 225.781 kb on + strand at 225.782 kb on - strand at 225.782 kb on - strand at 226.052 kb on - strand, within GFF1044 at 226.294 kb on - strand, within GFF1044 at 226.325 kb on + strand, within GFF1044 at 226.514 kb on - strand, within GFF1044 at 226.673 kb on + strand, within GFF1044 at 226.682 kb on + strand, within GFF1044 at 226.683 kb on - strand, within GFF1044 at 226.683 kb on - strand, within GFF1044 at 226.713 kb on + strand, within GFF1044 at 226.713 kb on + strand, within GFF1044 at 226.715 kb on + strand, within GFF1044 at 226.716 kb on - strand, within GFF1044 at 226.716 kb on - strand, within GFF1044 at 226.949 kb on - strand at 227.006 kb on + strand at 227.007 kb on - strand at 227.012 kb on + strand at 227.012 kb on + strand at 227.013 kb on - strand at 227.075 kb on - strand at 227.075 kb on - strand at 227.449 kb on + strand, within GFF1045 at 227.631 kb on - strand, within GFF1045 at 227.675 kb on + strand, within GFF1045 at 227.849 kb on + strand, within GFF1045 at 227.850 kb on - strand, within GFF1045 at 227.850 kb on - strand, within GFF1045
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 224,695 + -0.1 224,695 + -0.8 224,696 - +1.1 224,696 - +0.5 224,924 - GFF1043 0.18 +0.4 225,004 + GFF1043 0.28 +0.3 225,005 - GFF1043 0.28 +0.7 225,157 + GFF1043 0.48 +0.3 225,184 - GFF1043 0.51 +0.7 225,184 - GFF1043 0.51 +0.8 225,400 - GFF1043 0.79 -1.0 225,560 + -1.9 225,561 - +2.9 225,616 + +0.6 225,653 - -0.8 225,712 + +0.6 225,781 + +0.8 225,781 + +0.0 225,781 + -0.2 225,782 - +0.5 225,782 - +1.0 226,052 - GFF1044 0.28 +0.0 226,294 - GFF1044 0.46 +0.3 226,325 + GFF1044 0.48 -0.7 226,514 - GFF1044 0.63 +0.3 226,673 + GFF1044 0.75 -0.2 226,682 + GFF1044 0.75 -2.4 226,683 - GFF1044 0.75 -0.5 226,683 - GFF1044 0.75 +1.1 226,713 + GFF1044 0.78 -0.4 226,713 + GFF1044 0.78 +0.1 226,715 + GFF1044 0.78 +0.8 226,716 - GFF1044 0.78 +0.9 226,716 - GFF1044 0.78 +0.6 226,949 - +1.6 227,006 + -0.2 227,007 - +0.2 227,012 + +0.2 227,012 + -0.2 227,013 - +0.3 227,075 - +0.6 227,075 - +1.4 227,449 + GFF1045 0.32 -0.2 227,631 - GFF1045 0.47 -0.9 227,675 + GFF1045 0.50 -0.2 227,849 + GFF1045 0.64 -0.2 227,850 - GFF1045 0.65 -1.9 227,850 - GFF1045 0.65 -0.4
Or see this region's nucleotide sequence