Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF828

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF827 and GFF828 are separated by 2 nucleotidesGFF828 and GFF829 are separated by 78 nucleotidesGFF829 and GFF830 are separated by 102 nucleotides GFF827 - HflK protein, at 9,800 to 11,059 GFF827 GFF828 - HflC protein, at 11,062 to 12,066 GFF828 GFF829 - Putative inner membrane protein YjeT (clustered with HflC), at 12,145 to 12,342 GFF829 GFF830 - Adenylosuccinate synthetase (EC 6.3.4.4), at 12,445 to 13,743 GFF830 Position (kb) 11 12 13Strain fitness (log2 ratio) -1 0 1 2 3at 10.063 kb on - strand, within GFF827at 10.390 kb on + strand, within GFF827at 10.394 kb on + strand, within GFF827at 10.468 kb on + strand, within GFF827at 10.590 kb on - strand, within GFF827at 10.610 kb on + strand, within GFF827at 10.610 kb on + strand, within GFF827at 10.677 kb on - strand, within GFF827at 10.810 kb on + strand, within GFF827at 10.821 kb on + strand, within GFF827at 10.954 kb on + strandat 11.003 kb on - strandat 11.068 kb on - strandat 11.102 kb on + strandat 11.110 kb on + strandat 11.111 kb on - strandat 11.371 kb on + strand, within GFF828at 11.372 kb on - strand, within GFF828at 11.558 kb on + strand, within GFF828at 11.559 kb on - strand, within GFF828at 11.652 kb on + strand, within GFF828at 11.731 kb on + strand, within GFF828at 11.739 kb on + strand, within GFF828at 11.869 kb on - strand, within GFF828at 12.131 kb on + strandat 12.133 kb on + strandat 12.134 kb on - strandat 12.317 kb on - strand, within GFF829at 12.390 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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10,063 - GFF827 0.21 +0.7
10,390 + GFF827 0.47 +1.3
10,394 + GFF827 0.47 +1.3
10,468 + GFF827 0.53 +0.4
10,590 - GFF827 0.63 +0.7
10,610 + GFF827 0.64 +0.4
10,610 + GFF827 0.64 +1.6
10,677 - GFF827 0.70 +1.6
10,810 + GFF827 0.80 +1.5
10,821 + GFF827 0.81 +1.4
10,954 + +1.0
11,003 - +2.0
11,068 - +2.0
11,102 + +1.0
11,110 + +1.3
11,111 - +2.3
11,371 + GFF828 0.31 +1.4
11,372 - GFF828 0.31 +2.1
11,558 + GFF828 0.49 +1.4
11,559 - GFF828 0.49 +2.4
11,652 + GFF828 0.59 -0.3
11,731 + GFF828 0.67 +2.2
11,739 + GFF828 0.67 +1.7
11,869 - GFF828 0.80 +3.1
12,131 + -1.1
12,133 + -0.8
12,134 - -0.7
12,317 - GFF829 0.87 +0.6
12,390 - -0.0

Or see this region's nucleotide sequence