Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF561 and GFF562 are separated by 44 nucleotides GFF562 and GFF563 overlap by 4 nucleotides
GFF561 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 290,874 to 292,616
GFF561
GFF562 - Puative phophotriesterase, at 292,661 to 293,695
GFF562
GFF563 - Putative inner membrane protein, at 293,692 to 294,663
GFF563
Position (kb) 292
293
294 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 291.681 kb on + strand, within GFF561 at 291.682 kb on - strand, within GFF561 at 291.789 kb on - strand, within GFF561 at 291.891 kb on + strand, within GFF561 at 291.891 kb on + strand, within GFF561 at 291.892 kb on - strand, within GFF561 at 292.068 kb on - strand, within GFF561 at 292.095 kb on + strand, within GFF561 at 292.274 kb on + strand, within GFF561 at 292.283 kb on + strand, within GFF561 at 292.541 kb on + strand at 292.652 kb on - strand at 292.662 kb on + strand at 292.687 kb on + strand at 292.694 kb on - strand at 292.712 kb on - strand at 292.741 kb on - strand at 292.823 kb on + strand, within GFF562 at 292.953 kb on - strand, within GFF562 at 292.972 kb on + strand, within GFF562 at 293.104 kb on + strand, within GFF562 at 293.129 kb on - strand, within GFF562 at 293.129 kb on - strand, within GFF562 at 293.162 kb on - strand, within GFF562 at 293.254 kb on + strand, within GFF562 at 293.287 kb on + strand, within GFF562 at 293.288 kb on - strand, within GFF562 at 293.319 kb on + strand, within GFF562 at 293.443 kb on + strand, within GFF562 at 293.580 kb on + strand, within GFF562 at 293.580 kb on + strand, within GFF562 at 293.673 kb on - strand at 293.735 kb on + strand at 293.802 kb on - strand, within GFF563 at 293.896 kb on + strand, within GFF563 at 294.061 kb on - strand, within GFF563 at 294.061 kb on - strand, within GFF563 at 294.061 kb on - strand, within GFF563 at 294.532 kb on + strand, within GFF563 at 294.547 kb on - strand, within GFF563 at 294.647 kb on + strand at 294.648 kb on - strand at 294.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 291,681 + GFF561 0.46 +0.1 291,682 - GFF561 0.46 +0.2 291,789 - GFF561 0.52 +0.3 291,891 + GFF561 0.58 -1.0 291,891 + GFF561 0.58 -1.0 291,892 - GFF561 0.58 -0.4 292,068 - GFF561 0.69 -0.1 292,095 + GFF561 0.70 -1.9 292,274 + GFF561 0.80 -0.3 292,283 + GFF561 0.81 -0.4 292,541 + +0.3 292,652 - -0.6 292,662 + -0.5 292,687 + -1.0 292,694 - -0.7 292,712 - -0.3 292,741 - -0.7 292,823 + GFF562 0.16 -0.9 292,953 - GFF562 0.28 -0.8 292,972 + GFF562 0.30 -2.0 293,104 + GFF562 0.43 +0.0 293,129 - GFF562 0.45 -1.2 293,129 - GFF562 0.45 -0.5 293,162 - GFF562 0.48 -3.3 293,254 + GFF562 0.57 -0.4 293,287 + GFF562 0.60 -0.4 293,288 - GFF562 0.61 -1.0 293,319 + GFF562 0.64 +0.6 293,443 + GFF562 0.76 +0.1 293,580 + GFF562 0.89 -0.1 293,580 + GFF562 0.89 -0.2 293,673 - -0.4 293,735 + -0.6 293,802 - GFF563 0.11 +0.2 293,896 + GFF563 0.21 -0.6 294,061 - GFF563 0.38 +0.7 294,061 - GFF563 0.38 -0.5 294,061 - GFF563 0.38 -0.8 294,532 + GFF563 0.86 -1.1 294,547 - GFF563 0.88 -0.3 294,647 + +0.1 294,648 - -0.1 294,676 - -0.7
Or see this region's nucleotide sequence