Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF513 and GFF514 are separated by 12 nucleotides GFF514 and GFF515 overlap by 40 nucleotides GFF515 and GFF516 are separated by 210 nucleotides
GFF513 - Glutathione reductase (EC 1.8.1.7), at 243,133 to 244,485
GFF513
GFF514 - hypothetical protein, at 244,498 to 244,629
GFF514
GFF515 - Protein involved in catabolism of external DNA, at 244,590 to 245,432
GFF515
GFF516 - Putative phosphatase, at 245,643 to 246,914
GFF516
Position (kb) 244
245
246 Strain fitness (log2 ratio) -2
-1
0
1
2 at 243.635 kb on + strand, within GFF513 at 243.675 kb on + strand, within GFF513 at 243.675 kb on + strand, within GFF513 at 243.678 kb on + strand, within GFF513 at 243.956 kb on + strand, within GFF513 at 243.998 kb on - strand, within GFF513 at 244.028 kb on + strand, within GFF513 at 244.191 kb on - strand, within GFF513 at 244.198 kb on - strand, within GFF513 at 244.214 kb on + strand, within GFF513 at 244.214 kb on + strand, within GFF513 at 244.215 kb on - strand, within GFF513 at 244.289 kb on + strand, within GFF513 at 244.409 kb on + strand at 244.409 kb on + strand at 244.410 kb on - strand at 244.414 kb on - strand at 244.466 kb on + strand at 244.466 kb on + strand at 244.466 kb on + strand at 244.467 kb on - strand at 244.467 kb on - strand at 244.467 kb on - strand at 244.467 kb on - strand at 244.467 kb on - strand at 244.467 kb on - strand at 244.473 kb on - strand at 244.498 kb on - strand at 244.518 kb on - strand, within GFF514 at 244.554 kb on - strand, within GFF514 at 244.554 kb on - strand, within GFF514 at 244.715 kb on + strand, within GFF515 at 244.821 kb on - strand, within GFF515 at 244.858 kb on + strand, within GFF515 at 244.880 kb on + strand, within GFF515 at 244.882 kb on + strand, within GFF515 at 245.192 kb on - strand, within GFF515 at 245.232 kb on - strand, within GFF515 at 245.252 kb on - strand, within GFF515 at 245.262 kb on - strand, within GFF515 at 245.381 kb on + strand at 245.382 kb on - strand at 245.442 kb on + strand at 245.455 kb on - strand at 245.471 kb on - strand at 245.488 kb on + strand at 245.534 kb on + strand at 245.642 kb on - strand at 245.657 kb on + strand at 245.662 kb on + strand at 245.708 kb on + strand at 245.709 kb on - strand at 245.819 kb on - strand, within GFF516 at 245.819 kb on - strand, within GFF516 at 245.819 kb on - strand, within GFF516 at 246.144 kb on + strand, within GFF516 at 246.144 kb on + strand, within GFF516 at 246.149 kb on + strand, within GFF516 at 246.182 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.258 kb on - strand, within GFF516 at 246.258 kb on - strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.356 kb on + strand, within GFF516
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 243,635 + GFF513 0.37 -0.4 243,675 + GFF513 0.40 +0.2 243,675 + GFF513 0.40 +0.3 243,678 + GFF513 0.40 +0.4 243,956 + GFF513 0.61 +0.5 243,998 - GFF513 0.64 +1.9 244,028 + GFF513 0.66 +0.0 244,191 - GFF513 0.78 +0.7 244,198 - GFF513 0.79 +1.1 244,214 + GFF513 0.80 -0.1 244,214 + GFF513 0.80 +0.7 244,215 - GFF513 0.80 +0.5 244,289 + GFF513 0.85 +0.7 244,409 + +0.1 244,409 + +0.9 244,410 - +1.3 244,414 - +0.2 244,466 + -0.1 244,466 + +0.9 244,466 + +0.5 244,467 - +0.1 244,467 - +0.5 244,467 - +0.9 244,467 - +0.2 244,467 - +0.6 244,467 - +1.9 244,473 - +1.4 244,498 - -0.5 244,518 - GFF514 0.15 -0.1 244,554 - GFF514 0.42 +0.6 244,554 - GFF514 0.42 -0.3 244,715 + GFF515 0.15 -1.5 244,821 - GFF515 0.27 -0.3 244,858 + GFF515 0.32 -0.6 244,880 + GFF515 0.34 -0.0 244,882 + GFF515 0.35 +0.3 245,192 - GFF515 0.71 -0.6 245,232 - GFF515 0.76 -0.2 245,252 - GFF515 0.79 -0.8 245,262 - GFF515 0.80 +0.0 245,381 + +0.5 245,382 - +1.1 245,442 + +0.1 245,455 - -0.3 245,471 - -0.2 245,488 + -0.0 245,534 + -0.5 245,642 - -0.5 245,657 + -0.4 245,662 + -0.3 245,708 + -0.4 245,709 - +0.0 245,819 - GFF516 0.14 +0.7 245,819 - GFF516 0.14 -0.8 245,819 - GFF516 0.14 -1.5 246,144 + GFF516 0.39 -0.1 246,144 + GFF516 0.39 -0.3 246,149 + GFF516 0.40 -0.4 246,182 + GFF516 0.42 -0.2 246,257 + GFF516 0.48 -0.3 246,257 + GFF516 0.48 -1.8 246,257 + GFF516 0.48 +1.6 246,257 + GFF516 0.48 -1.1 246,258 - GFF516 0.48 -0.2 246,258 - GFF516 0.48 -0.9 246,356 + GFF516 0.56 -1.9 246,356 + GFF516 0.56 +0.1 246,356 + GFF516 0.56 -0.1
Or see this region's nucleotide sequence