Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4503 and GFF4504 are separated by 2 nucleotides GFF4504 and GFF4505 are separated by 181 nucleotides GFF4505 and GFF4506 are separated by 63 nucleotides GFF4506 and GFF4507 are separated by 35 nucleotides
GFF4503 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) of siderophore biosynthesis, at 290,135 to 290,890
GFF4503
GFF4504 - Proofreading thioesterase in enterobactin biosynthesis EntH, at 290,893 to 291,306
GFF4504
GFF4505 - Carbon starvation protein A, at 291,488 to 293,593
GFF4505
GFF4506 - COG2879, Hypothetical small protein yjiX, at 293,657 to 293,854
GFF4506
GFF4507 - Glycerol dehydrogenase (EC 1.1.1.6), at 293,890 to 294,978
GFF4507
Position (kb) 291
292
293
294 Strain fitness (log2 ratio) -1
0
1
2 at 290.548 kb on + strand, within GFF4503 at 290.563 kb on + strand, within GFF4503 at 290.584 kb on + strand, within GFF4503 at 290.651 kb on - strand, within GFF4503 at 290.842 kb on + strand at 290.842 kb on + strand at 290.843 kb on - strand at 290.974 kb on + strand, within GFF4504 at 290.975 kb on - strand, within GFF4504 at 291.103 kb on + strand, within GFF4504 at 291.296 kb on + strand at 291.376 kb on - strand at 291.439 kb on + strand at 291.439 kb on + strand at 291.439 kb on + strand at 291.439 kb on + strand at 291.493 kb on + strand at 291.494 kb on - strand at 291.506 kb on + strand at 291.554 kb on + strand at 291.557 kb on - strand at 291.557 kb on - strand at 291.557 kb on - strand at 291.620 kb on + strand at 291.620 kb on + strand at 291.621 kb on - strand at 291.626 kb on - strand at 291.650 kb on + strand at 291.651 kb on - strand at 291.652 kb on + strand at 291.688 kb on + strand at 291.689 kb on - strand at 291.709 kb on + strand, within GFF4505 at 291.725 kb on + strand, within GFF4505 at 291.886 kb on + strand, within GFF4505 at 292.067 kb on + strand, within GFF4505 at 292.067 kb on + strand, within GFF4505 at 292.067 kb on + strand, within GFF4505 at 292.068 kb on - strand, within GFF4505 at 292.113 kb on - strand, within GFF4505 at 292.186 kb on + strand, within GFF4505 at 292.230 kb on - strand, within GFF4505 at 292.274 kb on + strand, within GFF4505 at 292.480 kb on + strand, within GFF4505 at 292.480 kb on + strand, within GFF4505 at 292.637 kb on - strand, within GFF4505 at 292.639 kb on + strand, within GFF4505 at 292.739 kb on - strand, within GFF4505 at 292.808 kb on - strand, within GFF4505 at 292.861 kb on - strand, within GFF4505 at 293.051 kb on - strand, within GFF4505 at 293.443 kb on + strand at 293.477 kb on - strand at 293.600 kb on + strand at 293.608 kb on + strand at 293.688 kb on - strand, within GFF4506 at 293.738 kb on + strand, within GFF4506 at 293.738 kb on + strand, within GFF4506 at 293.738 kb on + strand, within GFF4506 at 293.739 kb on - strand, within GFF4506 at 293.739 kb on - strand, within GFF4506 at 293.739 kb on - strand, within GFF4506 at 293.786 kb on + strand, within GFF4506 at 293.786 kb on + strand, within GFF4506 at 293.787 kb on - strand, within GFF4506 at 293.787 kb on - strand, within GFF4506 at 293.894 kb on - strand at 293.960 kb on + strand at 293.988 kb on + strand at 294.230 kb on + strand, within GFF4507 at 294.460 kb on - strand, within GFF4507 at 294.482 kb on + strand, within GFF4507 at 294.482 kb on + strand, within GFF4507 at 294.483 kb on - strand, within GFF4507 at 294.582 kb on - strand, within GFF4507 at 294.582 kb on - strand, within GFF4507
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 290,548 + GFF4503 0.55 -0.0 290,563 + GFF4503 0.57 +0.9 290,584 + GFF4503 0.59 -0.4 290,651 - GFF4503 0.68 +0.2 290,842 + +1.3 290,842 + -0.6 290,843 - +0.1 290,974 + GFF4504 0.20 +0.9 290,975 - GFF4504 0.20 +0.3 291,103 + GFF4504 0.51 +0.5 291,296 + -0.2 291,376 - +0.0 291,439 + -0.0 291,439 + +0.4 291,439 + +0.1 291,439 + -0.3 291,493 + +0.5 291,494 - +2.3 291,506 + -0.0 291,554 + +0.0 291,557 - -0.2 291,557 - +0.1 291,557 - +0.1 291,620 + +0.5 291,620 + -0.0 291,621 - +1.2 291,626 - -0.2 291,650 + +0.6 291,651 - +0.9 291,652 + +0.7 291,688 + +0.2 291,689 - +0.6 291,709 + GFF4505 0.10 -1.6 291,725 + GFF4505 0.11 +0.2 291,886 + GFF4505 0.19 -0.3 292,067 + GFF4505 0.27 -0.1 292,067 + GFF4505 0.27 +0.1 292,067 + GFF4505 0.27 -0.9 292,068 - GFF4505 0.28 -0.2 292,113 - GFF4505 0.30 +2.0 292,186 + GFF4505 0.33 +0.3 292,230 - GFF4505 0.35 +0.4 292,274 + GFF4505 0.37 +0.2 292,480 + GFF4505 0.47 +2.6 292,480 + GFF4505 0.47 +0.3 292,637 - GFF4505 0.55 -0.6 292,639 + GFF4505 0.55 +0.2 292,739 - GFF4505 0.59 +0.5 292,808 - GFF4505 0.63 +0.2 292,861 - GFF4505 0.65 +0.3 293,051 - GFF4505 0.74 +0.6 293,443 + -0.1 293,477 - +0.2 293,600 + +1.9 293,608 + +0.6 293,688 - GFF4506 0.16 +0.8 293,738 + GFF4506 0.41 +0.9 293,738 + GFF4506 0.41 +0.8 293,738 + GFF4506 0.41 +1.2 293,739 - GFF4506 0.41 -0.1 293,739 - GFF4506 0.41 -0.1 293,739 - GFF4506 0.41 +0.4 293,786 + GFF4506 0.65 +0.7 293,786 + GFF4506 0.65 +0.5 293,787 - GFF4506 0.66 -1.6 293,787 - GFF4506 0.66 -0.2 293,894 - -0.5 293,960 + +0.6 293,988 + +1.0 294,230 + GFF4507 0.31 +0.5 294,460 - GFF4507 0.52 +0.4 294,482 + GFF4507 0.54 +0.8 294,482 + GFF4507 0.54 +1.0 294,483 - GFF4507 0.54 -0.8 294,582 - GFF4507 0.64 +0.4 294,582 - GFF4507 0.64 +0.1
Or see this region's nucleotide sequence