Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4476 and GFF4477 are separated by 66 nucleotides GFF4477 and GFF4478 are separated by 14 nucleotides GFF4478 and GFF4479 are separated by 2 nucleotides
GFF4476 - Putative phosphosugar isomerase, at 262,401 to 263,387
GFF4476
GFF4477 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 263,454 to 264,515
GFF4477
GFF4478 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 264,530 to 265,384
GFF4478
GFF4479 - PTS system, mannose-specific IIC component (EC 2.7.1.69), at 265,387 to 266,130
GFF4479
Position (kb) 263
264
265 Strain fitness (log2 ratio) -1
0
1
2 at 262.473 kb on - strand at 262.652 kb on + strand, within GFF4476 at 262.652 kb on + strand, within GFF4476 at 262.711 kb on - strand, within GFF4476 at 262.711 kb on - strand, within GFF4476 at 262.749 kb on - strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.801 kb on + strand, within GFF4476 at 262.802 kb on - strand, within GFF4476 at 262.854 kb on + strand, within GFF4476 at 262.928 kb on - strand, within GFF4476 at 263.027 kb on - strand, within GFF4476 at 263.038 kb on + strand, within GFF4476 at 263.217 kb on - strand, within GFF4476 at 263.727 kb on - strand, within GFF4477 at 263.758 kb on + strand, within GFF4477 at 263.759 kb on - strand, within GFF4477 at 264.117 kb on - strand, within GFF4477 at 264.122 kb on + strand, within GFF4477 at 264.125 kb on - strand, within GFF4477 at 264.125 kb on - strand, within GFF4477 at 264.321 kb on + strand, within GFF4477 at 264.395 kb on + strand, within GFF4477 at 264.396 kb on - strand, within GFF4477 at 264.497 kb on + strand at 264.498 kb on - strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.500 kb on - strand at 264.508 kb on - strand at 264.508 kb on - strand at 264.515 kb on + strand at 264.516 kb on - strand at 264.516 kb on - strand at 264.522 kb on - strand at 264.622 kb on - strand, within GFF4478 at 264.622 kb on - strand, within GFF4478 at 264.789 kb on + strand, within GFF4478 at 264.790 kb on - strand, within GFF4478 at 264.951 kb on + strand, within GFF4478 at 264.951 kb on + strand, within GFF4478 at 264.952 kb on - strand, within GFF4478 at 264.952 kb on - strand, within GFF4478 at 265.258 kb on - strand, within GFF4478 at 265.280 kb on - strand, within GFF4478 at 265.480 kb on - strand, within GFF4479 at 265.511 kb on + strand, within GFF4479 at 265.511 kb on + strand, within GFF4479 at 265.512 kb on - strand, within GFF4479 at 265.512 kb on - strand, within GFF4479
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 262,473 - +0.5 262,652 + GFF4476 0.25 -0.3 262,652 + GFF4476 0.25 -0.5 262,711 - GFF4476 0.31 +1.8 262,711 - GFF4476 0.31 -0.3 262,749 - GFF4476 0.35 -0.0 262,754 + GFF4476 0.36 -0.2 262,754 + GFF4476 0.36 -0.1 262,754 + GFF4476 0.36 +0.3 262,754 + GFF4476 0.36 -0.5 262,801 + GFF4476 0.41 -0.1 262,802 - GFF4476 0.41 +0.0 262,854 + GFF4476 0.46 -0.2 262,928 - GFF4476 0.53 +0.3 263,027 - GFF4476 0.63 -0.5 263,038 + GFF4476 0.65 +0.7 263,217 - GFF4476 0.83 -0.6 263,727 - GFF4477 0.26 +0.6 263,758 + GFF4477 0.29 -0.0 263,759 - GFF4477 0.29 +0.3 264,117 - GFF4477 0.62 +0.3 264,122 + GFF4477 0.63 +1.1 264,125 - GFF4477 0.63 +0.7 264,125 - GFF4477 0.63 +0.4 264,321 + GFF4477 0.82 -0.1 264,395 + GFF4477 0.89 +0.2 264,396 - GFF4477 0.89 +0.5 264,497 + +0.3 264,498 - +0.8 264,499 + +0.8 264,499 + +0.2 264,500 - +0.5 264,508 - -0.2 264,508 - +0.9 264,515 + -0.7 264,516 - +0.9 264,516 - +0.8 264,522 - -0.9 264,622 - GFF4478 0.11 +0.4 264,622 - GFF4478 0.11 -1.6 264,789 + GFF4478 0.30 +0.3 264,790 - GFF4478 0.30 +1.5 264,951 + GFF4478 0.49 +0.3 264,951 + GFF4478 0.49 +1.8 264,952 - GFF4478 0.49 +0.4 264,952 - GFF4478 0.49 -0.1 265,258 - GFF4478 0.85 +0.2 265,280 - GFF4478 0.88 -0.0 265,480 - GFF4479 0.12 -1.6 265,511 + GFF4479 0.17 +0.3 265,511 + GFF4479 0.17 -0.4 265,512 - GFF4479 0.17 +0.2 265,512 - GFF4479 0.17 +0.5
Or see this region's nucleotide sequence