Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4252 and GFF4253 are separated by 12 nucleotides GFF4253 and GFF4254 are separated by 494 nucleotides
GFF4252 - Type III restriction-modification system methylation subunit (EC 2.1.1.72), at 33,543 to 35,501
GFF4252
GFF4253 - Type III restriction-modification system StyLTI enzyme res (EC 3.1.21.5), at 35,514 to 38,483
GFF4253
GFF4254 - putative Cytochrome bd2, subunit I, at 38,978 to 40,381
GFF4254
Position (kb)
35
36
37
38
39 Strain fitness (log2 ratio)
-2
-1
0
1 at 34.514 kb on - strand, within GFF4252 at 34.622 kb on + strand, within GFF4252 at 34.875 kb on - strand, within GFF4252 at 34.925 kb on + strand, within GFF4252 at 34.926 kb on - strand, within GFF4252 at 34.926 kb on - strand, within GFF4252 at 34.931 kb on + strand, within GFF4252 at 34.932 kb on - strand, within GFF4252 at 35.084 kb on + strand, within GFF4252 at 35.097 kb on - strand, within GFF4252 at 35.243 kb on + strand, within GFF4252 at 35.243 kb on + strand, within GFF4252 at 35.296 kb on - strand, within GFF4252 at 35.301 kb on + strand, within GFF4252 at 35.376 kb on - strand at 35.409 kb on + strand at 35.410 kb on - strand at 35.499 kb on + strand at 35.500 kb on - strand at 35.520 kb on - strand at 35.520 kb on - strand at 35.616 kb on + strand at 35.617 kb on - strand at 35.621 kb on + strand at 35.660 kb on + strand at 35.697 kb on + strand at 35.698 kb on - strand at 35.703 kb on + strand at 35.704 kb on - strand at 35.704 kb on - strand at 35.704 kb on - strand at 35.718 kb on + strand at 35.718 kb on + strand at 35.719 kb on - strand at 35.736 kb on + strand at 35.736 kb on + strand at 35.736 kb on + strand at 35.737 kb on - strand at 35.804 kb on + strand at 35.804 kb on + strand at 35.804 kb on + strand at 35.964 kb on - strand, within GFF4253 at 36.098 kb on + strand, within GFF4253 at 36.099 kb on - strand, within GFF4253 at 36.129 kb on - strand, within GFF4253 at 36.139 kb on - strand, within GFF4253 at 36.224 kb on + strand, within GFF4253 at 36.243 kb on + strand, within GFF4253 at 36.312 kb on - strand, within GFF4253 at 36.312 kb on - strand, within GFF4253 at 36.318 kb on - strand, within GFF4253 at 36.321 kb on + strand, within GFF4253 at 36.321 kb on + strand, within GFF4253 at 36.360 kb on + strand, within GFF4253 at 36.363 kb on - strand, within GFF4253 at 36.813 kb on + strand, within GFF4253 at 36.813 kb on + strand, within GFF4253 at 36.813 kb on + strand, within GFF4253 at 36.814 kb on - strand, within GFF4253 at 36.929 kb on - strand, within GFF4253 at 37.069 kb on + strand, within GFF4253 at 37.070 kb on - strand, within GFF4253 at 37.070 kb on - strand, within GFF4253 at 37.132 kb on + strand, within GFF4253 at 37.132 kb on + strand, within GFF4253 at 37.132 kb on + strand, within GFF4253 at 37.133 kb on - strand, within GFF4253 at 37.198 kb on - strand, within GFF4253 at 37.459 kb on - strand, within GFF4253 at 37.563 kb on - strand, within GFF4253 at 37.594 kb on - strand, within GFF4253 at 37.594 kb on - strand, within GFF4253 at 37.595 kb on + strand, within GFF4253 at 37.595 kb on + strand, within GFF4253 at 37.595 kb on + strand, within GFF4253 at 37.596 kb on - strand, within GFF4253 at 37.668 kb on + strand, within GFF4253 at 37.859 kb on - strand, within GFF4253 at 37.926 kb on - strand, within GFF4253 at 37.926 kb on - strand, within GFF4253 at 37.980 kb on + strand, within GFF4253 at 38.051 kb on + strand, within GFF4253 at 38.120 kb on - strand, within GFF4253 at 38.237 kb on + strand at 38.370 kb on - strand at 38.575 kb on - strand at 38.619 kb on + strand at 38.619 kb on + strand at 38.619 kb on + strand at 38.742 kb on - strand at 38.768 kb on + strand at 38.768 kb on + strand at 38.772 kb on + strand at 38.776 kb on - strand at 38.872 kb on + strand at 38.872 kb on + strand at 38.959 kb on + strand at 38.961 kb on + strand at 38.961 kb on + strand at 39.097 kb on - strand at 39.250 kb on + strand, within GFF4254 at 39.367 kb on + strand, within GFF4254 at 39.374 kb on - strand, within GFF4254 at 39.471 kb on + strand, within GFF4254
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 34,514 - GFF4252 0.50 -0.3 34,622 + GFF4252 0.55 -1.2 34,875 - GFF4252 0.68 -0.2 34,925 + GFF4252 0.71 -0.6 34,926 - GFF4252 0.71 -0.1 34,926 - GFF4252 0.71 -0.2 34,931 + GFF4252 0.71 -0.8 34,932 - GFF4252 0.71 +0.4 35,084 + GFF4252 0.79 +0.2 35,097 - GFF4252 0.79 -1.0 35,243 + GFF4252 0.87 -0.8 35,243 + GFF4252 0.87 -0.2 35,296 - GFF4252 0.89 -0.8 35,301 + GFF4252 0.90 -1.6 35,376 - -0.4 35,409 + -0.5 35,410 - -1.1 35,499 + -0.4 35,500 - -0.2 35,520 - -0.6 35,520 - -1.2 35,616 + -1.1 35,617 - -0.2 35,621 + -0.8 35,660 + -0.8 35,697 + +0.7 35,698 - -0.4 35,703 + -0.4 35,704 - -0.9 35,704 - -0.8 35,704 - -0.2 35,718 + -1.4 35,718 + -1.0 35,719 - -0.5 35,736 + -1.2 35,736 + +1.6 35,736 + -2.4 35,737 - -0.1 35,804 + +0.2 35,804 + -0.8 35,804 + -1.1 35,964 - GFF4253 0.15 -0.9 36,098 + GFF4253 0.20 -0.9 36,099 - GFF4253 0.20 +0.0 36,129 - GFF4253 0.21 -0.2 36,139 - GFF4253 0.21 -0.2 36,224 + GFF4253 0.24 -1.1 36,243 + GFF4253 0.25 -0.1 36,312 - GFF4253 0.27 -0.7 36,312 - GFF4253 0.27 -1.2 36,318 - GFF4253 0.27 -0.3 36,321 + GFF4253 0.27 -0.6 36,321 + GFF4253 0.27 -0.8 36,360 + GFF4253 0.28 -1.0 36,363 - GFF4253 0.29 -0.6 36,813 + GFF4253 0.44 -1.1 36,813 + GFF4253 0.44 -0.6 36,813 + GFF4253 0.44 -1.0 36,814 - GFF4253 0.44 +0.3 36,929 - GFF4253 0.48 -1.4 37,069 + GFF4253 0.52 -0.4 37,070 - GFF4253 0.52 +0.0 37,070 - GFF4253 0.52 -1.4 37,132 + GFF4253 0.54 -0.7 37,132 + GFF4253 0.54 -0.5 37,132 + GFF4253 0.54 -1.2 37,133 - GFF4253 0.55 +0.1 37,198 - GFF4253 0.57 +0.5 37,459 - GFF4253 0.65 -0.9 37,563 - GFF4253 0.69 +0.2 37,594 - GFF4253 0.70 -0.3 37,594 - GFF4253 0.70 -0.6 37,595 + GFF4253 0.70 -0.5 37,595 + GFF4253 0.70 -0.7 37,595 + GFF4253 0.70 -0.8 37,596 - GFF4253 0.70 -1.0 37,668 + GFF4253 0.73 -0.2 37,859 - GFF4253 0.79 -0.6 37,926 - GFF4253 0.81 -0.7 37,926 - GFF4253 0.81 -0.2 37,980 + GFF4253 0.83 -0.8 38,051 + GFF4253 0.85 -0.6 38,120 - GFF4253 0.88 -0.6 38,237 + -0.1 38,370 - -0.4 38,575 - +0.4 38,619 + +0.4 38,619 + +0.0 38,619 + -0.2 38,742 - -0.2 38,768 + -0.5 38,768 + +0.2 38,772 + +0.2 38,776 - +0.0 38,872 + +0.7 38,872 + -0.3 38,959 + +0.3 38,961 + +0.2 38,961 + -1.0 39,097 - +0.6 39,250 + GFF4254 0.19 -0.0 39,367 + GFF4254 0.28 -0.3 39,374 - GFF4254 0.28 +0.4 39,471 + GFF4254 0.35 +0.0
Or see this region's nucleotide sequence