Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4152 and GFF4153 are separated by 600 nucleotides GFF4153 and GFF4154 overlap by 20 nucleotides
GFF4152 - Phage integrase, Phage P4-associated, at 31,956 to 33,020
GFF4152
GFF4153 - Type I secretion system, membrane fusion protein LapC, at 33,621 to 34,811
GFF4153
GFF4154 - Type I secretion system ATPase, LssB family LapB, at 34,792 to 36,972
GFF4154
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1
2 at 32.736 kb on + strand, within GFF4152 at 32.834 kb on - strand, within GFF4152 at 32.881 kb on + strand, within GFF4152 at 32.946 kb on + strand at 32.947 kb on - strand at 33.242 kb on - strand at 33.250 kb on - strand at 33.261 kb on - strand at 33.304 kb on - strand at 33.562 kb on - strand at 33.562 kb on - strand at 33.565 kb on + strand at 33.608 kb on + strand at 33.623 kb on - strand at 33.705 kb on + strand at 33.706 kb on - strand at 33.759 kb on + strand, within GFF4153 at 33.871 kb on + strand, within GFF4153 at 33.927 kb on - strand, within GFF4153 at 34.177 kb on + strand, within GFF4153 at 34.178 kb on - strand, within GFF4153 at 34.431 kb on + strand, within GFF4153 at 34.432 kb on - strand, within GFF4153 at 34.432 kb on - strand, within GFF4153 at 34.982 kb on - strand at 34.982 kb on - strand at 35.007 kb on + strand at 35.008 kb on - strand at 35.041 kb on + strand, within GFF4154 at 35.049 kb on + strand, within GFF4154 at 35.248 kb on + strand, within GFF4154 at 35.303 kb on - strand, within GFF4154 at 35.722 kb on - strand, within GFF4154 at 35.722 kb on - strand, within GFF4154 at 35.722 kb on - strand, within GFF4154 at 35.768 kb on + strand, within GFF4154 at 35.768 kb on + strand, within GFF4154 at 35.769 kb on - strand, within GFF4154 at 35.802 kb on + strand, within GFF4154
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 32,736 + GFF4152 0.73 -0.8 32,834 - GFF4152 0.82 -0.7 32,881 + GFF4152 0.87 -0.4 32,946 + +1.0 32,947 - -0.4 33,242 - +1.9 33,250 - +2.0 33,261 - +2.5 33,304 - +2.5 33,562 - +1.7 33,562 - +1.6 33,565 + +1.9 33,608 + +0.3 33,623 - +0.3 33,705 + +0.0 33,706 - -0.3 33,759 + GFF4153 0.12 +0.1 33,871 + GFF4153 0.21 +0.4 33,927 - GFF4153 0.26 -0.5 34,177 + GFF4153 0.47 +1.0 34,178 - GFF4153 0.47 -0.1 34,431 + GFF4153 0.68 -0.7 34,432 - GFF4153 0.68 +0.4 34,432 - GFF4153 0.68 +0.5 34,982 - +0.1 34,982 - -0.1 35,007 + +0.7 35,008 - -0.6 35,041 + GFF4154 0.11 -0.0 35,049 + GFF4154 0.12 +1.1 35,248 + GFF4154 0.21 +0.2 35,303 - GFF4154 0.23 +1.8 35,722 - GFF4154 0.43 +0.1 35,722 - GFF4154 0.43 +0.3 35,722 - GFF4154 0.43 +1.0 35,768 + GFF4154 0.45 -0.4 35,768 + GFF4154 0.45 -0.9 35,769 - GFF4154 0.45 -0.7 35,802 + GFF4154 0.46 -0.2
Or see this region's nucleotide sequence