Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4152

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4150 and GFF4151 are separated by 1 nucleotidesGFF4151 and GFF4152 are separated by 13 nucleotidesGFF4152 and GFF4153 are separated by 600 nucleotides GFF4150 - putative periplasmic protein, at 31,135 to 31,773 GFF4150 GFF4151 - integrase-like protein, at 31,775 to 31,942 GFF4151 GFF4152 - Phage integrase, Phage P4-associated, at 31,956 to 33,020 GFF4152 GFF4153 - Type I secretion system, membrane fusion protein LapC, at 33,621 to 34,811 GFF4153 Position (kb) 31 32 33 34Strain fitness (log2 ratio) -1 0 1 2at 30.990 kb on + strandat 30.991 kb on - strandat 31.043 kb on + strandat 31.043 kb on + strandat 31.072 kb on - strandat 31.238 kb on - strand, within GFF4150at 31.238 kb on - strand, within GFF4150at 31.477 kb on - strand, within GFF4150at 31.515 kb on + strand, within GFF4150at 31.550 kb on + strand, within GFF4150at 31.627 kb on - strand, within GFF4150at 31.631 kb on + strand, within GFF4150at 31.729 kb on - strandat 32.069 kb on + strand, within GFF4152at 32.069 kb on + strand, within GFF4152at 32.069 kb on + strand, within GFF4152at 32.287 kb on - strand, within GFF4152at 32.481 kb on - strand, within GFF4152at 32.736 kb on + strand, within GFF4152at 32.834 kb on - strand, within GFF4152at 32.881 kb on + strand, within GFF4152at 32.946 kb on + strandat 32.947 kb on - strandat 33.242 kb on - strandat 33.250 kb on - strandat 33.261 kb on - strandat 33.304 kb on - strandat 33.562 kb on - strandat 33.562 kb on - strandat 33.565 kb on + strandat 33.608 kb on + strandat 33.623 kb on - strandat 33.705 kb on + strandat 33.706 kb on - strandat 33.759 kb on + strand, within GFF4153at 33.871 kb on + strand, within GFF4153at 33.927 kb on - strand, within GFF4153

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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30,990 + +0.3
30,991 - -0.4
31,043 + -1.1
31,043 + +0.0
31,072 - -0.8
31,238 - GFF4150 0.16 -0.6
31,238 - GFF4150 0.16 -0.1
31,477 - GFF4150 0.54 +0.1
31,515 + GFF4150 0.59 +0.3
31,550 + GFF4150 0.65 +1.1
31,627 - GFF4150 0.77 +0.4
31,631 + GFF4150 0.78 -0.7
31,729 - -1.5
32,069 + GFF4152 0.11 -0.4
32,069 + GFF4152 0.11 -0.3
32,069 + GFF4152 0.11 -0.5
32,287 - GFF4152 0.31 -0.2
32,481 - GFF4152 0.49 -0.3
32,736 + GFF4152 0.73 -0.8
32,834 - GFF4152 0.82 -0.7
32,881 + GFF4152 0.87 -0.4
32,946 + +1.0
32,947 - -0.4
33,242 - +1.9
33,250 - +2.0
33,261 - +2.5
33,304 - +2.5
33,562 - +1.7
33,562 - +1.6
33,565 + +1.9
33,608 + +0.3
33,623 - +0.3
33,705 + +0.0
33,706 - -0.3
33,759 + GFF4153 0.12 +0.1
33,871 + GFF4153 0.21 +0.4
33,927 - GFF4153 0.26 -0.5

Or see this region's nucleotide sequence