Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF393

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF392 and GFF393 are separated by 202 nucleotidesGFF393 and GFF394 are separated by 9 nucleotides GFF392 - ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20), at 106,830 to 107,762 GFF392 GFF393 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 107,965 to 109,161 GFF393 GFF394 - L-threonine 3-dehydrogenase (EC 1.1.1.103), at 109,171 to 110,196 GFF394 Position (kb) 107 108 109 110Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 106.992 kb on - strand, within GFF392at 107.126 kb on - strand, within GFF392at 107.252 kb on - strand, within GFF392at 107.404 kb on + strand, within GFF392at 107.426 kb on - strand, within GFF392at 107.482 kb on + strand, within GFF392at 107.583 kb on - strand, within GFF392at 107.781 kb on - strandat 107.822 kb on - strandat 107.827 kb on + strandat 107.869 kb on + strandat 107.930 kb on + strandat 107.931 kb on - strandat 108.048 kb on + strandat 108.096 kb on + strand, within GFF393at 108.115 kb on - strand, within GFF393at 108.118 kb on + strand, within GFF393at 108.118 kb on + strand, within GFF393at 108.118 kb on + strand, within GFF393at 108.118 kb on + strand, within GFF393at 108.151 kb on - strand, within GFF393at 108.286 kb on + strand, within GFF393at 108.349 kb on - strand, within GFF393at 108.378 kb on + strand, within GFF393at 108.378 kb on - strand, within GFF393at 108.379 kb on - strand, within GFF393at 108.422 kb on - strand, within GFF393at 108.668 kb on + strand, within GFF393at 108.668 kb on + strand, within GFF393at 108.669 kb on - strand, within GFF393at 108.672 kb on + strand, within GFF393at 108.673 kb on - strand, within GFF393at 108.673 kb on - strand, within GFF393at 108.676 kb on - strand, within GFF393at 108.776 kb on - strand, within GFF393at 108.822 kb on + strand, within GFF393at 109.016 kb on - strand, within GFF393at 109.016 kb on - strand, within GFF393at 109.016 kb on - strand, within GFF393at 109.033 kb on + strand, within GFF393at 109.104 kb on + strandat 109.167 kb on + strandat 109.168 kb on - strandat 109.168 kb on - strandat 109.261 kb on - strandat 109.303 kb on + strand, within GFF394at 109.306 kb on - strand, within GFF394at 109.363 kb on + strand, within GFF394at 109.364 kb on - strand, within GFF394at 109.364 kb on - strand, within GFF394at 109.364 kb on - strand, within GFF394at 109.364 kb on - strand, within GFF394at 109.540 kb on + strand, within GFF394at 109.599 kb on - strand, within GFF394at 109.670 kb on + strand, within GFF394at 109.974 kb on + strand, within GFF394

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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106,992 - GFF392 0.17 +3.2
107,126 - GFF392 0.32 +4.0
107,252 - GFF392 0.45 +2.2
107,404 + GFF392 0.62 +2.2
107,426 - GFF392 0.64 +2.6
107,482 + GFF392 0.70 +2.1
107,583 - GFF392 0.81 +1.6
107,781 - +3.0
107,822 - -0.6
107,827 + +2.6
107,869 + -0.8
107,930 + +0.3
107,931 - -0.3
108,048 + -0.3
108,096 + GFF393 0.11 -0.3
108,115 - GFF393 0.13 -0.3
108,118 + GFF393 0.13 -0.4
108,118 + GFF393 0.13 -1.0
108,118 + GFF393 0.13 -0.1
108,118 + GFF393 0.13 -0.4
108,151 - GFF393 0.16 -0.6
108,286 + GFF393 0.27 -0.3
108,349 - GFF393 0.32 -0.8
108,378 + GFF393 0.35 -0.5
108,378 - GFF393 0.35 -2.4
108,379 - GFF393 0.35 -0.6
108,422 - GFF393 0.38 -2.4
108,668 + GFF393 0.59 -0.5
108,668 + GFF393 0.59 +0.2
108,669 - GFF393 0.59 -0.2
108,672 + GFF393 0.59 -0.8
108,673 - GFF393 0.59 +0.2
108,673 - GFF393 0.59 -0.2
108,676 - GFF393 0.59 -0.1
108,776 - GFF393 0.68 -0.4
108,822 + GFF393 0.72 -0.9
109,016 - GFF393 0.88 +0.1
109,016 - GFF393 0.88 +0.7
109,016 - GFF393 0.88 +0.9
109,033 + GFF393 0.89 -2.2
109,104 + -0.5
109,167 + -0.2
109,168 - -0.7
109,168 - -0.6
109,261 - -0.8
109,303 + GFF394 0.13 -1.1
109,306 - GFF394 0.13 +0.7
109,363 + GFF394 0.19 -0.1
109,364 - GFF394 0.19 -0.2
109,364 - GFF394 0.19 +0.2
109,364 - GFF394 0.19 -1.5
109,364 - GFF394 0.19 -0.4
109,540 + GFF394 0.36 -0.2
109,599 - GFF394 0.42 +0.7
109,670 + GFF394 0.49 -1.4
109,974 + GFF394 0.78 -0.6

Or see this region's nucleotide sequence