Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF387

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF385 and GFF386 are separated by 22 nucleotidesGFF386 and GFF387 are separated by 149 nucleotidesGFF387 and GFF388 are separated by 100 nucleotides GFF385 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58), at 99,581 to 100,591 GFF385 GFF386 - Lipopolysaccharide core biosynthesis protein RfaY, at 100,614 to 101,312 GFF386 GFF387 - Lipopolysaccharide core biosynthesis protein RfaZ, at 101,462 to 102,271 GFF387 GFF388 - Lipopolysaccharide 1,2-N-acetylglucosaminetransferase (EC 2.4.1.56), at 102,372 to 103,517 GFF388 Position (kb) 101 102 103Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 100.954 kb on - strand, within GFF386at 100.958 kb on - strand, within GFF386at 101.056 kb on + strand, within GFF386at 101.507 kb on - strandat 101.982 kb on - strand, within GFF387at 102.025 kb on + strand, within GFF387at 102.028 kb on - strand, within GFF387at 102.641 kb on + strand, within GFF388at 102.671 kb on + strand, within GFF388at 102.671 kb on + strand, within GFF388at 102.690 kb on - strand, within GFF388at 102.759 kb on - strand, within GFF388at 103.029 kb on - strand, within GFF388at 103.148 kb on + strand, within GFF388at 103.152 kb on - strand, within GFF388at 103.196 kb on + strand, within GFF388at 103.198 kb on + strand, within GFF388

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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100,954 - GFF386 0.49 -0.2
100,958 - GFF386 0.49 -0.5
101,056 + GFF386 0.63 -0.7
101,507 - +4.1
101,982 - GFF387 0.64 +4.0
102,025 + GFF387 0.70 -0.0
102,028 - GFF387 0.70 +3.8
102,641 + GFF388 0.23 +5.0
102,671 + GFF388 0.26 +5.2
102,671 + GFF388 0.26 +4.7
102,690 - GFF388 0.28 +5.6
102,759 - GFF388 0.34 +6.3
103,029 - GFF388 0.57 +4.9
103,148 + GFF388 0.68 +4.9
103,152 - GFF388 0.68 +4.3
103,196 + GFF388 0.72 +4.8
103,198 + GFF388 0.72 +6.3

Or see this region's nucleotide sequence