Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3799 and GFF3800 are separated by 11 nucleotides GFF3800 and GFF3801 are separated by 15 nucleotides GFF3801 and GFF3802 are separated by 8 nucleotides
GFF3799 - 4-hydroxybenzoate transporter, at 68,096 to 69,454
GFF3799
GFF3800 - Gentisate 1,2-dioxygenase (EC 1.13.11.4), at 69,466 to 70,503
GFF3800
GFF3801 - Fumarylacetoacetase (EC 3.7.1.2), at 70,519 to 71,220
GFF3801
GFF3802 - Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18), at 71,229 to 71,873
GFF3802
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-1
0
1
2 at 68.561 kb on - strand, within GFF3799 at 68.561 kb on - strand, within GFF3799 at 68.950 kb on + strand, within GFF3799 at 69.072 kb on - strand, within GFF3799 at 69.072 kb on - strand, within GFF3799 at 69.102 kb on + strand, within GFF3799 at 69.164 kb on - strand, within GFF3799 at 69.326 kb on - strand at 69.488 kb on + strand at 69.520 kb on - strand at 69.632 kb on + strand, within GFF3800 at 69.740 kb on - strand, within GFF3800 at 69.749 kb on + strand, within GFF3800 at 69.813 kb on + strand, within GFF3800 at 70.045 kb on + strand, within GFF3800 at 70.046 kb on - strand, within GFF3800 at 70.123 kb on + strand, within GFF3800 at 70.308 kb on + strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.312 kb on + strand, within GFF3800 at 70.312 kb on + strand, within GFF3800 at 70.501 kb on + strand at 70.528 kb on + strand at 70.621 kb on + strand, within GFF3801 at 70.622 kb on - strand, within GFF3801 at 70.760 kb on - strand, within GFF3801 at 70.765 kb on + strand, within GFF3801 at 70.766 kb on - strand, within GFF3801 at 70.794 kb on + strand, within GFF3801 at 70.835 kb on - strand, within GFF3801 at 70.959 kb on - strand, within GFF3801 at 70.981 kb on - strand, within GFF3801 at 70.999 kb on - strand, within GFF3801 at 71.080 kb on + strand, within GFF3801 at 71.184 kb on - strand at 71.184 kb on - strand at 71.272 kb on + strand at 71.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 68,561 - GFF3799 0.34 +0.8 68,561 - GFF3799 0.34 +0.2 68,950 + GFF3799 0.63 +0.5 69,072 - GFF3799 0.72 +0.1 69,072 - GFF3799 0.72 +2.3 69,102 + GFF3799 0.74 -0.2 69,164 - GFF3799 0.79 +0.7 69,326 - +0.2 69,488 + +0.9 69,520 - -0.2 69,632 + GFF3800 0.16 -0.2 69,740 - GFF3800 0.26 +0.4 69,749 + GFF3800 0.27 +0.7 69,813 + GFF3800 0.33 -0.0 70,045 + GFF3800 0.56 +0.4 70,046 - GFF3800 0.56 -0.1 70,123 + GFF3800 0.63 +1.6 70,308 + GFF3800 0.81 -0.5 70,309 - GFF3800 0.81 +0.5 70,309 - GFF3800 0.81 +1.3 70,309 - GFF3800 0.81 -0.4 70,309 - GFF3800 0.81 +1.7 70,312 + GFF3800 0.82 -0.3 70,312 + GFF3800 0.82 +2.1 70,501 + +0.3 70,528 + -0.2 70,621 + GFF3801 0.15 +0.4 70,622 - GFF3801 0.15 -0.4 70,760 - GFF3801 0.34 +0.3 70,765 + GFF3801 0.35 +0.1 70,766 - GFF3801 0.35 +0.2 70,794 + GFF3801 0.39 -0.8 70,835 - GFF3801 0.45 -0.5 70,959 - GFF3801 0.63 -0.3 70,981 - GFF3801 0.66 +0.1 70,999 - GFF3801 0.68 -0.0 71,080 + GFF3801 0.80 +0.2 71,184 - +1.0 71,184 - +0.4 71,272 + +0.0 71,277 - -0.3
Or see this region's nucleotide sequence