Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3778 and GFF3779 are separated by 384 nucleotides GFF3779 and GFF3780 are separated by 43 nucleotides
GFF3778 - Lysine-specific permease, at 44,494 to 45,963
GFF3778
GFF3779 - Colicin I receptor precursor, at 46,348 to 48,300
GFF3779
GFF3780 - Hexose phosphate uptake regulatory protein UhpC, at 48,344 to 49,666
GFF3780
Position (kb) 46
47
48
49 Strain fitness (log2 ratio) -2
-1
0
1
2 at 45.430 kb on - strand, within GFF3778 at 45.443 kb on - strand, within GFF3778 at 45.463 kb on + strand, within GFF3778 at 45.464 kb on - strand, within GFF3778 at 45.706 kb on + strand, within GFF3778 at 45.786 kb on + strand, within GFF3778 at 45.802 kb on + strand, within GFF3778 at 45.802 kb on + strand, within GFF3778 at 45.854 kb on - strand at 45.861 kb on + strand at 45.902 kb on + strand at 45.970 kb on - strand at 45.997 kb on - strand at 45.997 kb on - strand at 46.208 kb on + strand at 46.250 kb on + strand at 46.286 kb on + strand at 46.348 kb on - strand at 46.458 kb on + strand at 46.459 kb on - strand at 46.578 kb on + strand, within GFF3779 at 46.579 kb on - strand, within GFF3779 at 46.633 kb on - strand, within GFF3779 at 46.705 kb on - strand, within GFF3779 at 46.858 kb on + strand, within GFF3779 at 47.176 kb on - strand, within GFF3779 at 47.270 kb on - strand, within GFF3779 at 47.458 kb on - strand, within GFF3779 at 47.585 kb on + strand, within GFF3779 at 47.709 kb on - strand, within GFF3779 at 47.869 kb on + strand, within GFF3779 at 47.869 kb on + strand, within GFF3779 at 47.870 kb on - strand, within GFF3779 at 47.959 kb on + strand, within GFF3779 at 47.959 kb on + strand, within GFF3779 at 47.959 kb on + strand, within GFF3779 at 47.960 kb on - strand, within GFF3779 at 47.960 kb on - strand, within GFF3779 at 47.960 kb on - strand, within GFF3779 at 47.961 kb on + strand, within GFF3779 at 48.076 kb on + strand, within GFF3779 at 48.091 kb on + strand, within GFF3779 at 48.251 kb on - strand at 48.347 kb on + strand at 48.411 kb on + strand at 48.412 kb on - strand at 48.415 kb on + strand at 48.416 kb on - strand at 48.417 kb on + strand at 48.579 kb on + strand, within GFF3780 at 48.624 kb on + strand, within GFF3780 at 48.663 kb on - strand, within GFF3780 at 48.663 kb on - strand, within GFF3780 at 48.733 kb on - strand, within GFF3780 at 48.797 kb on + strand, within GFF3780 at 48.889 kb on + strand, within GFF3780 at 48.920 kb on - strand, within GFF3780 at 48.921 kb on + strand, within GFF3780 at 48.922 kb on - strand, within GFF3780 at 49.022 kb on + strand, within GFF3780 at 49.028 kb on - strand, within GFF3780 at 49.028 kb on - strand, within GFF3780 at 49.049 kb on - strand, within GFF3780 at 49.049 kb on - strand, within GFF3780 at 49.235 kb on - strand, within GFF3780
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 45,430 - GFF3778 0.64 -0.1 45,443 - GFF3778 0.65 -0.3 45,463 + GFF3778 0.66 -1.0 45,464 - GFF3778 0.66 -0.1 45,706 + GFF3778 0.82 -0.0 45,786 + GFF3778 0.88 -0.3 45,802 + GFF3778 0.89 +0.6 45,802 + GFF3778 0.89 -0.5 45,854 - +0.0 45,861 + -0.6 45,902 + +0.5 45,970 - -1.3 45,997 - -0.3 45,997 - +0.8 46,208 + -1.2 46,250 + -0.5 46,286 + -0.0 46,348 - -0.7 46,458 + +0.4 46,459 - +0.5 46,578 + GFF3779 0.12 -0.5 46,579 - GFF3779 0.12 -0.0 46,633 - GFF3779 0.15 -0.3 46,705 - GFF3779 0.18 -0.9 46,858 + GFF3779 0.26 +0.2 47,176 - GFF3779 0.42 +0.4 47,270 - GFF3779 0.47 +0.2 47,458 - GFF3779 0.57 +0.4 47,585 + GFF3779 0.63 -1.0 47,709 - GFF3779 0.70 -0.4 47,869 + GFF3779 0.78 -0.3 47,869 + GFF3779 0.78 -2.3 47,870 - GFF3779 0.78 -0.3 47,959 + GFF3779 0.82 -0.5 47,959 + GFF3779 0.82 -0.3 47,959 + GFF3779 0.82 +0.1 47,960 - GFF3779 0.83 +0.2 47,960 - GFF3779 0.83 +0.2 47,960 - GFF3779 0.83 -0.3 47,961 + GFF3779 0.83 -1.6 48,076 + GFF3779 0.88 -2.0 48,091 + GFF3779 0.89 -0.1 48,251 - -1.2 48,347 + +2.3 48,411 + -0.8 48,412 - -0.9 48,415 + +0.4 48,416 - -0.8 48,417 + -1.1 48,579 + GFF3780 0.18 -0.2 48,624 + GFF3780 0.21 +0.5 48,663 - GFF3780 0.24 -0.6 48,663 - GFF3780 0.24 -0.0 48,733 - GFF3780 0.29 -0.3 48,797 + GFF3780 0.34 +0.7 48,889 + GFF3780 0.41 -0.5 48,920 - GFF3780 0.44 +0.1 48,921 + GFF3780 0.44 -0.2 48,922 - GFF3780 0.44 +1.6 49,022 + GFF3780 0.51 -0.6 49,028 - GFF3780 0.52 +0.4 49,028 - GFF3780 0.52 +0.1 49,049 - GFF3780 0.53 -0.7 49,049 - GFF3780 0.53 +1.0 49,235 - GFF3780 0.67 -0.1
Or see this region's nucleotide sequence