Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3695 and GFF3696 are separated by 194 nucleotides GFF3696 and GFF3697 overlap by 4 nucleotides
GFF3695 - Nicotinate phosphoribosyltransferase (EC 2.4.2.11), at 48,431 to 49,633
GFF3695
GFF3696 - Integrase, at 49,828 to 51,168
GFF3696
GFF3697 - Phage excisionase, at 51,165 to 51,413
GFF3697
Position (kb) 49
50
51
52 Strain fitness (log2 ratio) -2
-1
0
1
2 at 48.853 kb on + strand, within GFF3695 at 48.853 kb on + strand, within GFF3695 at 49.071 kb on - strand, within GFF3695 at 49.178 kb on - strand, within GFF3695 at 49.277 kb on - strand, within GFF3695 at 49.277 kb on - strand, within GFF3695 at 49.310 kb on - strand, within GFF3695 at 49.310 kb on - strand, within GFF3695 at 49.355 kb on - strand, within GFF3695 at 49.485 kb on + strand, within GFF3695 at 49.486 kb on - strand, within GFF3695 at 49.486 kb on - strand, within GFF3695 at 49.542 kb on + strand at 49.543 kb on - strand at 49.581 kb on + strand at 49.633 kb on + strand at 49.633 kb on + strand at 49.703 kb on - strand at 50.024 kb on + strand, within GFF3696 at 50.024 kb on + strand, within GFF3696 at 50.025 kb on - strand, within GFF3696 at 50.029 kb on - strand, within GFF3696 at 50.288 kb on + strand, within GFF3696 at 50.288 kb on + strand, within GFF3696 at 50.290 kb on + strand, within GFF3696 at 50.290 kb on + strand, within GFF3696 at 50.291 kb on - strand, within GFF3696 at 50.304 kb on + strand, within GFF3696 at 50.389 kb on - strand, within GFF3696 at 50.436 kb on - strand, within GFF3696 at 50.436 kb on - strand, within GFF3696 at 50.627 kb on + strand, within GFF3696 at 50.627 kb on + strand, within GFF3696 at 50.628 kb on - strand, within GFF3696 at 50.628 kb on - strand, within GFF3696 at 50.720 kb on + strand, within GFF3696 at 50.720 kb on + strand, within GFF3696 at 50.721 kb on - strand, within GFF3696 at 50.805 kb on + strand, within GFF3696 at 50.806 kb on - strand, within GFF3696 at 50.940 kb on + strand, within GFF3696 at 51.046 kb on - strand at 51.046 kb on - strand at 51.052 kb on - strand at 51.053 kb on + strand at 51.054 kb on - strand at 51.235 kb on + strand, within GFF3697 at 51.235 kb on + strand, within GFF3697 at 51.238 kb on - strand, within GFF3697 at 51.238 kb on - strand, within GFF3697 at 51.238 kb on - strand, within GFF3697 at 51.287 kb on - strand, within GFF3697
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 48,853 + GFF3695 0.35 +0.6 48,853 + GFF3695 0.35 +0.5 49,071 - GFF3695 0.53 +1.1 49,178 - GFF3695 0.62 +0.5 49,277 - GFF3695 0.70 +0.4 49,277 - GFF3695 0.70 +0.4 49,310 - GFF3695 0.73 +0.8 49,310 - GFF3695 0.73 -0.3 49,355 - GFF3695 0.77 -0.4 49,485 + GFF3695 0.88 -1.1 49,486 - GFF3695 0.88 +0.5 49,486 - GFF3695 0.88 +0.9 49,542 + +0.1 49,543 - -0.0 49,581 + +0.4 49,633 + +1.7 49,633 + +0.3 49,703 - -2.0 50,024 + GFF3696 0.15 -0.1 50,024 + GFF3696 0.15 -0.5 50,025 - GFF3696 0.15 -0.2 50,029 - GFF3696 0.15 +0.0 50,288 + GFF3696 0.34 -0.5 50,288 + GFF3696 0.34 +1.7 50,290 + GFF3696 0.34 -1.4 50,290 + GFF3696 0.34 -0.0 50,291 - GFF3696 0.35 -0.8 50,304 + GFF3696 0.35 -0.2 50,389 - GFF3696 0.42 -0.4 50,436 - GFF3696 0.45 -1.4 50,436 - GFF3696 0.45 +0.2 50,627 + GFF3696 0.60 +0.2 50,627 + GFF3696 0.60 +0.7 50,628 - GFF3696 0.60 -0.9 50,628 - GFF3696 0.60 +2.2 50,720 + GFF3696 0.67 -0.3 50,720 + GFF3696 0.67 +0.2 50,721 - GFF3696 0.67 -1.0 50,805 + GFF3696 0.73 -0.1 50,806 - GFF3696 0.73 -0.2 50,940 + GFF3696 0.83 -1.4 51,046 - -0.3 51,046 - -0.3 51,052 - +0.2 51,053 + -0.4 51,054 - -0.6 51,235 + GFF3697 0.28 -0.0 51,235 + GFF3697 0.28 -0.8 51,238 - GFF3697 0.29 -1.1 51,238 - GFF3697 0.29 -0.7 51,238 - GFF3697 0.29 -0.4 51,287 - GFF3697 0.49 -0.8
Or see this region's nucleotide sequence