Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3433 and GFF3434 are separated by 148 nucleotides GFF3434 and GFF3435 are separated by 338 nucleotides GFF3435 and GFF3436 are separated by 82 nucleotides GFF3436 and GFF3437 are separated by 148 nucleotides
GFF3433 - FIG074102: hypothetical protein, at 197,294 to 197,416
GFF3433
GFF3434 - Small heat shock protein, at 197,565 to 198,035
GFF3434
GFF3435 - FIG01046570: hypothetical protein, at 198,374 to 199,417
GFF3435
GFF3436 - Cytochrome C553 (soluble cytochrome f), at 199,500 to 200,030
GFF3436
GFF3437 - Putative outer membrane lipoprotein, at 200,179 to 200,493
GFF3437
Position (kb) 198
199
200 Strain fitness (log2 ratio) -2
-1
0
1 at 197.502 kb on + strand at 197.502 kb on + strand at 197.502 kb on + strand at 197.724 kb on + strand, within GFF3434 at 197.724 kb on + strand, within GFF3434 at 197.724 kb on + strand, within GFF3434 at 197.798 kb on + strand, within GFF3434 at 198.122 kb on + strand at 198.142 kb on + strand at 198.142 kb on + strand at 198.146 kb on - strand at 198.244 kb on - strand at 198.264 kb on + strand at 198.305 kb on + strand at 198.356 kb on + strand at 198.551 kb on - strand, within GFF3435 at 198.551 kb on - strand, within GFF3435 at 198.553 kb on + strand, within GFF3435 at 198.554 kb on - strand, within GFF3435 at 198.887 kb on - strand, within GFF3435 at 199.107 kb on - strand, within GFF3435 at 199.156 kb on + strand, within GFF3435 at 199.336 kb on + strand at 199.356 kb on + strand at 199.357 kb on - strand at 199.360 kb on + strand at 199.361 kb on - strand at 199.367 kb on + strand at 199.393 kb on - strand at 199.393 kb on - strand at 199.393 kb on - strand at 199.497 kb on + strand at 199.550 kb on - strand at 199.622 kb on + strand, within GFF3436 at 199.629 kb on + strand, within GFF3436 at 199.630 kb on - strand, within GFF3436 at 199.676 kb on + strand, within GFF3436 at 199.847 kb on - strand, within GFF3436 at 199.891 kb on + strand, within GFF3436 at 199.892 kb on - strand, within GFF3436 at 199.955 kb on + strand, within GFF3436 at 200.005 kb on - strand at 200.137 kb on - strand at 200.292 kb on + strand, within GFF3437 at 200.311 kb on + strand, within GFF3437 at 200.311 kb on + strand, within GFF3437 at 200.311 kb on + strand, within GFF3437 at 200.312 kb on - strand, within GFF3437
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 197,502 + -0.2 197,502 + +0.1 197,502 + -0.4 197,724 + GFF3434 0.34 -0.4 197,724 + GFF3434 0.34 +1.0 197,724 + GFF3434 0.34 +0.1 197,798 + GFF3434 0.49 -0.8 198,122 + -0.4 198,142 + +0.1 198,142 + +0.1 198,146 - +0.3 198,244 - +0.6 198,264 + +0.3 198,305 + -0.1 198,356 + -0.3 198,551 - GFF3435 0.17 +0.7 198,551 - GFF3435 0.17 +0.5 198,553 + GFF3435 0.17 -0.4 198,554 - GFF3435 0.17 +1.2 198,887 - GFF3435 0.49 +0.1 199,107 - GFF3435 0.70 -0.0 199,156 + GFF3435 0.75 -0.9 199,336 + -1.0 199,356 + -1.8 199,357 - +0.2 199,360 + -1.0 199,361 - +0.0 199,367 + +0.5 199,393 - -0.8 199,393 - +0.0 199,393 - -0.0 199,497 + -1.1 199,550 - -0.3 199,622 + GFF3436 0.23 -0.8 199,629 + GFF3436 0.24 -0.4 199,630 - GFF3436 0.24 +0.5 199,676 + GFF3436 0.33 +0.0 199,847 - GFF3436 0.65 -1.4 199,891 + GFF3436 0.74 -0.6 199,892 - GFF3436 0.74 +0.2 199,955 + GFF3436 0.86 -0.7 200,005 - -0.6 200,137 - +0.0 200,292 + GFF3437 0.36 -0.3 200,311 + GFF3437 0.42 +0.2 200,311 + GFF3437 0.42 -1.6 200,311 + GFF3437 0.42 -0.6 200,312 - GFF3437 0.42 -0.8
Or see this region's nucleotide sequence