Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3363

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3362 and GFF3363 are separated by 64 nucleotidesGFF3363 and GFF3364 are separated by 14 nucleotides GFF3362 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 126,505 to 127,455 GFF3362 GFF3363 - Flagellar hook-associated protein FlgK, at 127,520 to 129,181 GFF3363 GFF3364 - Flagellar hook-associated protein FlgL, at 129,196 to 130,149 GFF3364 Position (kb) 127 128 129 130Strain fitness (log2 ratio) -1 0 1 2 3at 126.520 kb on - strandat 127.220 kb on - strand, within GFF3362at 127.241 kb on + strand, within GFF3362at 127.280 kb on - strand, within GFF3362at 127.454 kb on - strandat 127.555 kb on + strandat 127.579 kb on + strandat 127.579 kb on + strandat 127.580 kb on - strandat 127.621 kb on + strandat 127.636 kb on + strandat 127.684 kb on + strandat 127.707 kb on + strand, within GFF3363at 127.718 kb on + strand, within GFF3363at 127.718 kb on + strand, within GFF3363at 127.718 kb on + strand, within GFF3363at 127.743 kb on + strand, within GFF3363at 127.743 kb on + strand, within GFF3363at 127.941 kb on + strand, within GFF3363at 128.175 kb on + strand, within GFF3363at 128.193 kb on - strand, within GFF3363at 128.193 kb on - strand, within GFF3363at 128.218 kb on + strand, within GFF3363at 128.218 kb on + strand, within GFF3363at 128.298 kb on - strand, within GFF3363at 128.298 kb on - strand, within GFF3363at 128.404 kb on + strand, within GFF3363at 128.405 kb on - strand, within GFF3363at 128.408 kb on - strand, within GFF3363at 128.527 kb on + strand, within GFF3363at 128.527 kb on + strand, within GFF3363at 128.527 kb on + strand, within GFF3363at 128.528 kb on - strand, within GFF3363at 128.536 kb on + strand, within GFF3363at 128.539 kb on + strand, within GFF3363at 128.539 kb on + strand, within GFF3363at 128.606 kb on + strand, within GFF3363at 128.608 kb on + strand, within GFF3363at 128.608 kb on + strand, within GFF3363at 128.919 kb on + strand, within GFF3363at 128.919 kb on + strand, within GFF3363at 128.920 kb on - strand, within GFF3363at 129.028 kb on + strandat 129.028 kb on + strandat 129.040 kb on + strandat 129.113 kb on + strandat 129.133 kb on - strandat 129.220 kb on + strandat 129.347 kb on + strand, within GFF3364at 129.379 kb on + strand, within GFF3364at 129.595 kb on + strand, within GFF3364at 129.676 kb on - strand, within GFF3364at 130.063 kb on + strandat 130.064 kb on - strandat 130.085 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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126,520 - +2.3
127,220 - GFF3362 0.75 +1.3
127,241 + GFF3362 0.77 -0.7
127,280 - GFF3362 0.81 +1.1
127,454 - -1.5
127,555 + +1.8
127,579 + +0.6
127,579 + +2.0
127,580 - +1.0
127,621 + +1.8
127,636 + +2.6
127,684 + +2.1
127,707 + GFF3363 0.11 +1.9
127,718 + GFF3363 0.12 +1.3
127,718 + GFF3363 0.12 +0.7
127,718 + GFF3363 0.12 +1.1
127,743 + GFF3363 0.13 +0.7
127,743 + GFF3363 0.13 +1.6
127,941 + GFF3363 0.25 +2.2
128,175 + GFF3363 0.39 +0.1
128,193 - GFF3363 0.40 -0.3
128,193 - GFF3363 0.40 +2.4
128,218 + GFF3363 0.42 +1.0
128,218 + GFF3363 0.42 +2.6
128,298 - GFF3363 0.47 +1.6
128,298 - GFF3363 0.47 -0.6
128,404 + GFF3363 0.53 +1.0
128,405 - GFF3363 0.53 +1.9
128,408 - GFF3363 0.53 -0.0
128,527 + GFF3363 0.61 +2.1
128,527 + GFF3363 0.61 +1.8
128,527 + GFF3363 0.61 +0.5
128,528 - GFF3363 0.61 +1.5
128,536 + GFF3363 0.61 +1.7
128,539 + GFF3363 0.61 +1.7
128,539 + GFF3363 0.61 +1.4
128,606 + GFF3363 0.65 -0.3
128,608 + GFF3363 0.65 +1.6
128,608 + GFF3363 0.65 +1.2
128,919 + GFF3363 0.84 +1.4
128,919 + GFF3363 0.84 +1.4
128,920 - GFF3363 0.84 +3.6
129,028 + +1.3
129,028 + +1.3
129,040 + +0.3
129,113 + +1.6
129,133 - +0.3
129,220 + +1.5
129,347 + GFF3364 0.16 +0.9
129,379 + GFF3364 0.19 -0.2
129,595 + GFF3364 0.42 +0.6
129,676 - GFF3364 0.50 +2.2
130,063 + +1.5
130,064 - -0.3
130,085 + +1.4

Or see this region's nucleotide sequence