Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3349 and GFF3350 are separated by 81 nucleotides GFF3350 and GFF3351 are separated by 34 nucleotides GFF3351 and GFF3352 are separated by 0 nucleotides GFF3352 and GFF3353 overlap by 26 nucleotides
GFF3349 - Proposed peptidoglycan lipid II flippase MurJ, at 117,015 to 118,589
GFF3349
GFF3350 - Flagellar biosynthesis protein FlgN, at 118,671 to 119,063
GFF3350
GFF3351 - Negative regulator of flagellin synthesis FlgM, at 119,098 to 119,391
GFF3351
GFF3352 - hypothetical protein, at 119,392 to 119,508
GFF3352
GFF3353 - Flagellar basal-body P-ring formation protein FlgA, at 119,483 to 120,142
GFF3353
Position (kb) 118
119
120 Strain fitness (log2 ratio) -1
0
1
2
3 at 118.624 kb on - strand at 118.729 kb on + strand, within GFF3350 at 118.729 kb on + strand, within GFF3350 at 118.967 kb on + strand, within GFF3350 at 118.971 kb on - strand, within GFF3350 at 118.977 kb on + strand, within GFF3350 at 119.094 kb on - strand at 119.100 kb on - strand at 119.100 kb on - strand at 119.104 kb on - strand at 119.104 kb on - strand at 119.115 kb on - strand at 119.215 kb on - strand, within GFF3351 at 119.582 kb on - strand, within GFF3353 at 119.639 kb on + strand, within GFF3353 at 119.675 kb on + strand, within GFF3353 at 119.676 kb on - strand, within GFF3353 at 119.683 kb on + strand, within GFF3353
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 118,624 - +0.3 118,729 + GFF3350 0.15 +0.1 118,729 + GFF3350 0.15 -0.2 118,967 + GFF3350 0.75 +3.4 118,971 - GFF3350 0.76 +1.1 118,977 + GFF3350 0.78 +0.4 119,094 - -0.1 119,100 - +0.6 119,100 - -0.7 119,104 - -0.2 119,104 - -0.3 119,115 - -0.7 119,215 - GFF3351 0.40 +0.9 119,582 - GFF3353 0.15 +1.1 119,639 + GFF3353 0.24 +0.1 119,675 + GFF3353 0.29 +1.3 119,676 - GFF3353 0.29 +1.4 119,683 + GFF3353 0.30 +1.3
Or see this region's nucleotide sequence