Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3334 and GFF3335 are separated by 51 nucleotides GFF3335 and GFF3336 overlap by 4 nucleotides GFF3336 and GFF3337 are separated by 10 nucleotides GFF3337 and GFF3338 are separated by 48 nucleotides GFF3338 and GFF3339 are separated by 40 nucleotides
GFF3334 - Rhodanese domain protein, Enterobacterial subgroup, YceA homolog, at 105,807 to 106,859
GFF3334
GFF3335 - Protein yceI precursor, at 106,911 to 107,486
GFF3335
GFF3336 - Cytochrome B561, at 107,483 to 108,055
GFF3336
GFF3337 - FIG01045781: hypothetical protein, at 108,066 to 108,269
GFF3337
GFF3338 - hypothetical protein, at 108,318 to 108,431
GFF3338
GFF3339 - N-methyl-L-amino-acid oxidase (EC 1.5.3.2); N-methyl-L-tryptophan oxidase (EC 1.5.3.-), at 108,472 to 109,590
GFF3339
Position (kb)
107
108
109 Strain fitness (log2 ratio)
-1
0
1 at 106.826 kb on + strand at 106.858 kb on - strand at 106.932 kb on + strand at 106.933 kb on - strand at 106.943 kb on + strand at 107.093 kb on + strand, within GFF3335 at 107.124 kb on - strand, within GFF3335 at 107.564 kb on + strand, within GFF3336 at 107.593 kb on + strand, within GFF3336 at 107.593 kb on + strand, within GFF3336 at 107.594 kb on - strand, within GFF3336 at 107.662 kb on + strand, within GFF3336 at 107.812 kb on - strand, within GFF3336 at 107.900 kb on - strand, within GFF3336 at 107.937 kb on + strand, within GFF3336 at 108.071 kb on + strand at 108.334 kb on + strand, within GFF3338 at 108.436 kb on + strand at 108.473 kb on + strand at 108.666 kb on - strand, within GFF3339 at 108.670 kb on - strand, within GFF3339 at 108.686 kb on - strand, within GFF3339 at 108.901 kb on - strand, within GFF3339
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 106,826 + +0.1 106,858 - +0.3 106,932 + +1.6 106,933 - -0.3 106,943 + -0.2 107,093 + GFF3335 0.32 -0.6 107,124 - GFF3335 0.37 -1.7 107,564 + GFF3336 0.14 -0.3 107,593 + GFF3336 0.19 +0.5 107,593 + GFF3336 0.19 +0.5 107,594 - GFF3336 0.19 +1.5 107,662 + GFF3336 0.31 +0.3 107,812 - GFF3336 0.57 -0.2 107,900 - GFF3336 0.73 +0.6 107,937 + GFF3336 0.79 +0.4 108,071 + -0.3 108,334 + GFF3338 0.14 +0.7 108,436 + +0.8 108,473 + +0.4 108,666 - GFF3339 0.17 +0.4 108,670 - GFF3339 0.18 +0.2 108,686 - GFF3339 0.19 +0.6 108,901 - GFF3339 0.38 +1.3
Or see this region's nucleotide sequence