Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF329

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF328 and GFF329 overlap by 14 nucleotidesGFF329 and GFF330 are separated by 235 nucleotides GFF328 - L-seryl-tRNA(Sec) selenium transferase-related protein, at 43,425 to 44,534 GFF328 GFF329 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16), at 44,521 to 45,264 GFF329 GFF330 - Transposase, at 45,500 to 46,441 GFF330 Position (kb) 44 45 46Strain fitness (log2 ratio) -1 0 1at 43.607 kb on + strand, within GFF328at 43.622 kb on + strand, within GFF328at 43.623 kb on - strand, within GFF328at 43.623 kb on - strand, within GFF328at 43.623 kb on - strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.742 kb on - strand, within GFF328at 43.760 kb on + strand, within GFF328at 44.045 kb on + strand, within GFF328at 44.049 kb on + strand, within GFF328at 44.050 kb on - strand, within GFF328at 44.050 kb on - strand, within GFF328at 44.070 kb on - strand, within GFF328at 44.099 kb on + strand, within GFF328at 44.099 kb on + strand, within GFF328at 44.099 kb on + strand, within GFF328at 44.190 kb on + strand, within GFF328at 44.191 kb on - strand, within GFF328at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.657 kb on + strand, within GFF329at 44.657 kb on + strand, within GFF329at 44.743 kb on + strand, within GFF329at 44.743 kb on + strand, within GFF329at 44.746 kb on + strand, within GFF329at 44.773 kb on - strand, within GFF329at 45.041 kb on + strand, within GFF329at 45.041 kb on + strand, within GFF329at 45.041 kb on + strand, within GFF329at 45.042 kb on - strand, within GFF329at 45.245 kb on + strandat 45.246 kb on - strandat 45.313 kb on + strandat 45.314 kb on - strandat 45.314 kb on - strandat 45.382 kb on - strandat 45.382 kb on - strandat 45.606 kb on + strand, within GFF330at 45.725 kb on + strand, within GFF330at 45.728 kb on - strand, within GFF330at 45.728 kb on - strand, within GFF330at 45.728 kb on - strand, within GFF330at 45.731 kb on + strand, within GFF330at 45.731 kb on + strand, within GFF330at 45.732 kb on - strand, within GFF330at 45.739 kb on + strand, within GFF330at 45.740 kb on - strand, within GFF330at 45.833 kb on - strand, within GFF330at 45.866 kb on + strand, within GFF330at 45.970 kb on - strand, within GFF330at 45.970 kb on - strand, within GFF330at 46.046 kb on - strand, within GFF330at 46.046 kb on - strand, within GFF330at 46.047 kb on + strand, within GFF330at 46.048 kb on - strand, within GFF330at 46.048 kb on - strand, within GFF330at 46.251 kb on + strand, within GFF330at 46.251 kb on + strand, within GFF330at 46.251 kb on + strand, within GFF330

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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43,607 + GFF328 0.16 -0.6
43,622 + GFF328 0.18 +0.1
43,623 - GFF328 0.18 +0.1
43,623 - GFF328 0.18 -0.8
43,623 - GFF328 0.18 -0.4
43,626 + GFF328 0.18 +0.3
43,626 + GFF328 0.18 -0.5
43,626 + GFF328 0.18 -0.5
43,626 + GFF328 0.18 +0.7
43,626 + GFF328 0.18 +0.2
43,626 + GFF328 0.18 +0.5
43,626 + GFF328 0.18 +0.1
43,626 + GFF328 0.18 +0.3
43,627 - GFF328 0.18 -0.2
43,627 - GFF328 0.18 +0.3
43,627 - GFF328 0.18 -0.2
43,627 - GFF328 0.18 +0.2
43,742 - GFF328 0.29 +0.4
43,760 + GFF328 0.30 +0.1
44,045 + GFF328 0.56 -0.6
44,049 + GFF328 0.56 +0.9
44,050 - GFF328 0.56 +0.3
44,050 - GFF328 0.56 +0.2
44,070 - GFF328 0.58 -0.8
44,099 + GFF328 0.61 +0.3
44,099 + GFF328 0.61 +0.4
44,099 + GFF328 0.61 -0.2
44,190 + GFF328 0.69 -0.2
44,191 - GFF328 0.69 +0.2
44,600 - GFF329 0.11 +0.5
44,600 - GFF329 0.11 -0.2
44,600 - GFF329 0.11 -0.6
44,600 - GFF329 0.11 +0.2
44,600 - GFF329 0.11 +0.2
44,657 + GFF329 0.18 +0.4
44,657 + GFF329 0.18 -0.1
44,743 + GFF329 0.30 +0.4
44,743 + GFF329 0.30 +0.1
44,746 + GFF329 0.30 -0.8
44,773 - GFF329 0.34 +0.8
45,041 + GFF329 0.70 -0.2
45,041 + GFF329 0.70 -0.5
45,041 + GFF329 0.70 -0.1
45,042 - GFF329 0.70 +0.9
45,245 + -0.6
45,246 - +0.6
45,313 + +0.3
45,314 - +0.8
45,314 - +1.0
45,382 - +0.5
45,382 - +0.1
45,606 + GFF330 0.11 -0.3
45,725 + GFF330 0.24 +0.0
45,728 - GFF330 0.24 +0.9
45,728 - GFF330 0.24 +0.1
45,728 - GFF330 0.24 -0.7
45,731 + GFF330 0.25 -0.3
45,731 + GFF330 0.25 -0.4
45,732 - GFF330 0.25 -0.3
45,739 + GFF330 0.25 +1.2
45,740 - GFF330 0.25 +1.7
45,833 - GFF330 0.35 +0.4
45,866 + GFF330 0.39 -0.8
45,970 - GFF330 0.50 +0.1
45,970 - GFF330 0.50 -0.8
46,046 - GFF330 0.58 +0.2
46,046 - GFF330 0.58 +0.2
46,047 + GFF330 0.58 -0.8
46,048 - GFF330 0.58 +1.2
46,048 - GFF330 0.58 +0.5
46,251 + GFF330 0.80 +0.4
46,251 + GFF330 0.80 -0.4
46,251 + GFF330 0.80 +0.2

Or see this region's nucleotide sequence