Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2736 and GFF2737 are separated by 223 nucleotides GFF2737 and GFF2738 are separated by 43 nucleotides GFF2738 and GFF2739 are separated by 178 nucleotides GFF2739 and GFF2740 are separated by 33 nucleotides GFF2740 and GFF2741 are separated by 47 nucleotides
GFF2736 - Ferric siderophore transport system, periplasmic binding protein TonB, at 256,878 to 257,606
GFF2736
GFF2737 - YciL protein, at 257,830 to 258,126
GFF2737
GFF2738 - hypothetical protein, at 258,170 to 258,307
GFF2738
GFF2739 - Cardiolipin synthetase (EC 2.7.8.-), at 258,486 to 259,946
GFF2739
GFF2740 - FIG002901: hypothetical protein co-occurring with Cardiolipin synthetase, at 259,980 to 260,309
GFF2740
GFF2741 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 260,357 to 261,193
GFF2741
Position (kb) 258
259
260 Strain fitness (log2 ratio) -2
-1
0
1
2 at 257.593 kb on - strand at 257.700 kb on - strand at 257.836 kb on + strand at 257.845 kb on + strand at 257.940 kb on - strand, within GFF2737 at 257.973 kb on + strand, within GFF2737 at 258.110 kb on - strand at 258.236 kb on - strand, within GFF2738 at 258.236 kb on - strand, within GFF2738 at 258.248 kb on - strand, within GFF2738 at 258.335 kb on - strand at 258.618 kb on + strand at 259.182 kb on + strand, within GFF2739 at 259.340 kb on + strand, within GFF2739 at 259.376 kb on + strand, within GFF2739 at 259.377 kb on - strand, within GFF2739 at 259.477 kb on - strand, within GFF2739 at 259.544 kb on + strand, within GFF2739 at 259.584 kb on - strand, within GFF2739 at 259.693 kb on + strand, within GFF2739 at 259.693 kb on + strand, within GFF2739 at 259.784 kb on + strand, within GFF2739 at 259.864 kb on + strand at 259.905 kb on - strand at 260.387 kb on - strand at 260.514 kb on + strand, within GFF2741 at 260.515 kb on - strand, within GFF2741 at 260.546 kb on - strand, within GFF2741 at 260.546 kb on - strand, within GFF2741 at 260.627 kb on - strand, within GFF2741 at 260.659 kb on + strand, within GFF2741 at 260.664 kb on + strand, within GFF2741 at 260.665 kb on - strand, within GFF2741
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 257,593 - -0.4 257,700 - +0.1 257,836 + +0.2 257,845 + -0.7 257,940 - GFF2737 0.37 +0.8 257,973 + GFF2737 0.48 +2.5 258,110 - -0.3 258,236 - GFF2738 0.48 +0.4 258,236 - GFF2738 0.48 -0.7 258,248 - GFF2738 0.57 +0.7 258,335 - -0.2 258,618 + +0.3 259,182 + GFF2739 0.48 +0.2 259,340 + GFF2739 0.58 +0.4 259,376 + GFF2739 0.61 -2.0 259,377 - GFF2739 0.61 +0.6 259,477 - GFF2739 0.68 -0.1 259,544 + GFF2739 0.72 -0.1 259,584 - GFF2739 0.75 +1.9 259,693 + GFF2739 0.83 +0.6 259,693 + GFF2739 0.83 -0.1 259,784 + GFF2739 0.89 +0.9 259,864 + +0.2 259,905 - -0.2 260,387 - -1.1 260,514 + GFF2741 0.19 +0.4 260,515 - GFF2741 0.19 +0.5 260,546 - GFF2741 0.23 +0.2 260,546 - GFF2741 0.23 +0.8 260,627 - GFF2741 0.32 -0.7 260,659 + GFF2741 0.36 +0.6 260,664 + GFF2741 0.37 +0.7 260,665 - GFF2741 0.37 -2.0
Or see this region's nucleotide sequence