Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2679 and GFF2680 are separated by 147 nucleotides GFF2680 and GFF2681 overlap by 4 nucleotides
GFF2679 - Transcriptional repressor protein TyrR, at 201,798 to 203,339
GFF2679
GFF2680 - Membrane protein YcjF, at 203,487 to 204,548
GFF2680
GFF2681 - Conserved protein YcjX with nucleoside triphosphate hydrolase domain, at 204,545 to 205,942
GFF2681
Position (kb) 203
204
205 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 202.516 kb on - strand, within GFF2679 at 202.644 kb on - strand, within GFF2679 at 202.722 kb on - strand, within GFF2679 at 202.722 kb on - strand, within GFF2679 at 202.722 kb on - strand, within GFF2679 at 202.957 kb on - strand, within GFF2679 at 203.048 kb on + strand, within GFF2679 at 203.268 kb on - strand at 203.272 kb on - strand at 203.281 kb on - strand at 203.369 kb on - strand at 203.369 kb on - strand at 203.369 kb on - strand at 203.420 kb on + strand at 203.507 kb on + strand at 203.574 kb on - strand at 203.574 kb on - strand at 203.605 kb on - strand, within GFF2680 at 203.791 kb on + strand, within GFF2680 at 203.791 kb on + strand, within GFF2680 at 203.794 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.868 kb on - strand, within GFF2680 at 204.025 kb on + strand, within GFF2680 at 204.117 kb on - strand, within GFF2680 at 204.120 kb on + strand, within GFF2680 at 204.289 kb on - strand, within GFF2680 at 204.444 kb on - strand at 204.444 kb on - strand at 204.492 kb on - strand at 204.557 kb on - strand at 204.566 kb on + strand at 204.610 kb on + strand at 204.832 kb on + strand, within GFF2681 at 204.833 kb on - strand, within GFF2681 at 204.833 kb on - strand, within GFF2681 at 204.871 kb on + strand, within GFF2681 at 204.872 kb on - strand, within GFF2681 at 204.895 kb on + strand, within GFF2681 at 204.896 kb on - strand, within GFF2681 at 205.039 kb on + strand, within GFF2681 at 205.155 kb on + strand, within GFF2681 at 205.158 kb on + strand, within GFF2681 at 205.334 kb on - strand, within GFF2681 at 205.464 kb on - strand, within GFF2681 at 205.493 kb on + strand, within GFF2681
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 202,516 - GFF2679 0.47 +0.4 202,644 - GFF2679 0.55 -1.0 202,722 - GFF2679 0.60 -0.4 202,722 - GFF2679 0.60 -1.1 202,722 - GFF2679 0.60 -2.1 202,957 - GFF2679 0.75 -1.0 203,048 + GFF2679 0.81 -0.7 203,268 - -0.5 203,272 - -0.1 203,281 - -0.6 203,369 - -0.8 203,369 - +0.0 203,369 - -0.3 203,420 + -0.2 203,507 + +0.2 203,574 - -1.2 203,574 - +0.2 203,605 - GFF2680 0.11 -0.2 203,791 + GFF2680 0.29 -0.1 203,791 + GFF2680 0.29 -0.1 203,794 + GFF2680 0.29 +1.3 203,812 + GFF2680 0.31 -0.5 203,812 + GFF2680 0.31 +0.7 203,812 + GFF2680 0.31 +3.3 203,868 - GFF2680 0.36 -0.3 204,025 + GFF2680 0.51 +1.1 204,117 - GFF2680 0.59 +1.0 204,120 + GFF2680 0.60 +0.8 204,289 - GFF2680 0.76 +0.3 204,444 - +1.2 204,444 - +0.6 204,492 - +0.2 204,557 - +0.4 204,566 + -0.4 204,610 + +0.3 204,832 + GFF2681 0.21 +0.1 204,833 - GFF2681 0.21 -0.1 204,833 - GFF2681 0.21 +0.8 204,871 + GFF2681 0.23 -0.1 204,872 - GFF2681 0.23 +1.8 204,895 + GFF2681 0.25 +0.6 204,896 - GFF2681 0.25 -0.2 205,039 + GFF2681 0.35 -0.1 205,155 + GFF2681 0.44 -0.4 205,158 + GFF2681 0.44 -0.4 205,334 - GFF2681 0.56 -0.1 205,464 - GFF2681 0.66 +0.5 205,493 + GFF2681 0.68 -0.2
Or see this region's nucleotide sequence