Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF257 and GFF258 are separated by 315 nucleotides GFF258 and GFF259 are separated by 139 nucleotides
GFF257 - Putative carboxymethylenebutenolidase (EC 3.1.1.45), at 32,627 to 33,382
GFF257
GFF258 - Uridine phosphorylase (EC 2.4.2.3), at 33,698 to 34,459
GFF258
GFF259 - DNA recombination protein RmuC, at 34,599 to 36,029
GFF259
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1 at 32.726 kb on - strand, within GFF257 at 32.749 kb on - strand, within GFF257 at 32.951 kb on + strand, within GFF257 at 32.951 kb on + strand, within GFF257 at 32.952 kb on - strand, within GFF257 at 32.973 kb on + strand, within GFF257 at 32.974 kb on - strand, within GFF257 at 32.974 kb on - strand, within GFF257 at 32.984 kb on - strand, within GFF257 at 32.984 kb on - strand, within GFF257 at 33.095 kb on + strand, within GFF257 at 33.128 kb on + strand, within GFF257 at 33.129 kb on - strand, within GFF257 at 33.130 kb on + strand, within GFF257 at 33.316 kb on + strand at 33.443 kb on - strand at 33.475 kb on + strand at 33.479 kb on + strand at 33.479 kb on + strand at 33.481 kb on + strand at 33.608 kb on + strand at 33.647 kb on - strand at 33.695 kb on + strand at 33.696 kb on - strand at 33.696 kb on - strand at 33.697 kb on + strand at 33.895 kb on + strand, within GFF258 at 33.958 kb on + strand, within GFF258 at 33.958 kb on + strand, within GFF258 at 33.958 kb on + strand, within GFF258 at 33.959 kb on - strand, within GFF258 at 34.211 kb on + strand, within GFF258 at 34.247 kb on - strand, within GFF258 at 34.247 kb on - strand, within GFF258 at 34.298 kb on - strand, within GFF258 at 34.457 kb on + strand at 34.607 kb on + strand at 34.607 kb on + strand at 34.608 kb on - strand at 34.608 kb on - strand at 34.613 kb on + strand at 34.613 kb on + strand at 34.614 kb on - strand at 34.737 kb on - strand at 34.796 kb on + strand, within GFF259 at 34.799 kb on + strand, within GFF259 at 34.823 kb on + strand, within GFF259 at 34.823 kb on + strand, within GFF259 at 34.826 kb on + strand, within GFF259 at 34.826 kb on + strand, within GFF259 at 34.826 kb on + strand, within GFF259 at 34.826 kb on + strand, within GFF259 at 34.827 kb on - strand, within GFF259 at 34.827 kb on - strand, within GFF259 at 34.827 kb on - strand, within GFF259 at 35.018 kb on + strand, within GFF259 at 35.142 kb on - strand, within GFF259 at 35.199 kb on - strand, within GFF259 at 35.268 kb on + strand, within GFF259 at 35.292 kb on - strand, within GFF259 at 35.422 kb on - strand, within GFF259 at 35.422 kb on - strand, within GFF259 at 35.441 kb on - strand, within GFF259
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 32,726 - GFF257 0.13 -0.7 32,749 - GFF257 0.16 -0.4 32,951 + GFF257 0.43 -0.4 32,951 + GFF257 0.43 -0.3 32,952 - GFF257 0.43 -0.1 32,973 + GFF257 0.46 -0.5 32,974 - GFF257 0.46 -0.6 32,974 - GFF257 0.46 -0.7 32,984 - GFF257 0.47 -0.3 32,984 - GFF257 0.47 -0.9 33,095 + GFF257 0.62 -0.2 33,128 + GFF257 0.66 -0.4 33,129 - GFF257 0.66 -0.4 33,130 + GFF257 0.67 +0.0 33,316 + -0.9 33,443 - -0.1 33,475 + -0.2 33,479 + -0.7 33,479 + -1.2 33,481 + -0.9 33,608 + -0.5 33,647 - +0.1 33,695 + -0.1 33,696 - +1.0 33,696 - +0.3 33,697 + -1.0 33,895 + GFF258 0.26 -0.5 33,958 + GFF258 0.34 -0.4 33,958 + GFF258 0.34 -0.4 33,958 + GFF258 0.34 -0.3 33,959 - GFF258 0.34 -0.7 34,211 + GFF258 0.67 +0.2 34,247 - GFF258 0.72 +0.1 34,247 - GFF258 0.72 -0.2 34,298 - GFF258 0.79 -0.2 34,457 + +0.2 34,607 + +0.2 34,607 + -0.5 34,608 - -0.1 34,608 - +0.1 34,613 + -0.1 34,613 + +1.5 34,614 - -0.8 34,737 - +0.2 34,796 + GFF259 0.14 +0.4 34,799 + GFF259 0.14 +0.0 34,823 + GFF259 0.16 -0.1 34,823 + GFF259 0.16 -0.8 34,826 + GFF259 0.16 +0.1 34,826 + GFF259 0.16 +0.9 34,826 + GFF259 0.16 -0.2 34,826 + GFF259 0.16 -0.4 34,827 - GFF259 0.16 +0.4 34,827 - GFF259 0.16 +0.1 34,827 - GFF259 0.16 +0.2 35,018 + GFF259 0.29 -0.8 35,142 - GFF259 0.38 -0.9 35,199 - GFF259 0.42 +0.1 35,268 + GFF259 0.47 -0.4 35,292 - GFF259 0.48 +0.3 35,422 - GFF259 0.58 -0.2 35,422 - GFF259 0.58 +0.2 35,441 - GFF259 0.59 -0.4
Or see this region's nucleotide sequence