Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2504 and GFF2505 are separated by 118 nucleotides GFF2505 and GFF2506 are separated by 28 nucleotides GFF2506 and GFF2507 are separated by 19 nucleotides GFF2507 and GFF2508 are separated by 315 nucleotides
GFF2504 - Permease of the drug/metabolite transporter (DMT) superfamily, at 21,446 to 22,348
GFF2504
GFF2505 - Multiple antibiotic resistance protein MarB, at 22,467 to 22,682
GFF2505
GFF2506 - Multiple antibiotic resistance protein MarA, at 22,711 to 23,094
GFF2506
GFF2507 - Multiple antibiotic resistance protein MarR, at 23,114 to 23,491
GFF2507
GFF2508 - Multiple antibiotic resistance protein MarC, at 23,807 to 24,472
GFF2508
Position (kb)
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 22.296 kb on - strand at 22.297 kb on + strand at 22.327 kb on + strand at 22.355 kb on - strand at 22.362 kb on + strand at 22.560 kb on + strand, within GFF2505 at 22.712 kb on + strand at 22.713 kb on - strand at 22.715 kb on + strand at 22.741 kb on - strand at 22.741 kb on - strand at 22.762 kb on - strand, within GFF2506 at 22.844 kb on + strand, within GFF2506 at 22.845 kb on - strand, within GFF2506 at 22.925 kb on + strand, within GFF2506 at 23.064 kb on + strand at 23.064 kb on + strand at 23.065 kb on - strand at 23.065 kb on - strand at 23.065 kb on - strand at 23.071 kb on - strand at 23.116 kb on - strand at 23.116 kb on - strand at 23.139 kb on + strand at 23.180 kb on + strand, within GFF2507 at 23.387 kb on - strand, within GFF2507 at 23.387 kb on - strand, within GFF2507 at 23.387 kb on - strand, within GFF2507 at 23.387 kb on - strand, within GFF2507 at 23.659 kb on - strand at 23.793 kb on + strand at 23.800 kb on + strand at 23.803 kb on + strand at 23.803 kb on + strand at 23.806 kb on + strand at 23.940 kb on - strand, within GFF2508 at 23.942 kb on - strand, within GFF2508 at 23.969 kb on - strand, within GFF2508 at 24.181 kb on + strand, within GFF2508 at 24.349 kb on + strand, within GFF2508 at 24.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 22,296 - +1.6 22,297 + -0.6 22,327 + -0.4 22,355 - +0.2 22,362 + -0.6 22,560 + GFF2505 0.43 +0.4 22,712 + +3.0 22,713 - +0.0 22,715 + +0.3 22,741 - +1.5 22,741 - -0.2 22,762 - GFF2506 0.13 +0.3 22,844 + GFF2506 0.35 -0.5 22,845 - GFF2506 0.35 -0.4 22,925 + GFF2506 0.56 +0.1 23,064 + +0.0 23,064 + +0.2 23,065 - -1.1 23,065 - -0.1 23,065 - -0.3 23,071 - -0.4 23,116 - -0.7 23,116 - -0.3 23,139 + +0.8 23,180 + GFF2507 0.17 -0.1 23,387 - GFF2507 0.72 -0.9 23,387 - GFF2507 0.72 -2.2 23,387 - GFF2507 0.72 +0.0 23,387 - GFF2507 0.72 -0.2 23,659 - +0.3 23,793 + -0.4 23,800 + +0.9 23,803 + +0.2 23,803 + +0.1 23,806 + +0.8 23,940 - GFF2508 0.20 +0.2 23,942 - GFF2508 0.20 +0.8 23,969 - GFF2508 0.24 +2.0 24,181 + GFF2508 0.56 -0.1 24,349 + GFF2508 0.81 -0.9 24,470 + +0.2
Or see this region's nucleotide sequence