Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2223 and GFF2224 are separated by 54 nucleotides GFF2224 and GFF2225 overlap by 16 nucleotides GFF2225 and GFF2226 overlap by 4 nucleotides
GFF2223 - GTP-binding protein TypA/BipA, at 16,308 to 18,131
GFF2223
GFF2224 - Putative hydrolase, at 18,186 to 18,920
GFF2224
GFF2225 - FIG01046022: hypothetical protein, at 18,905 to 19,783
GFF2225
GFF2226 - Hypothetical radical SAM family enzyme in interesting gene cluster, at 19,780 to 21,021
GFF2226
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1 at 17.911 kb on + strand, within GFF2223 at 18.129 kb on + strand at 18.242 kb on + strand at 18.242 kb on + strand at 18.341 kb on + strand, within GFF2224 at 18.397 kb on + strand, within GFF2224 at 18.398 kb on - strand, within GFF2224 at 18.422 kb on + strand, within GFF2224 at 18.423 kb on - strand, within GFF2224 at 18.423 kb on - strand, within GFF2224 at 18.467 kb on + strand, within GFF2224 at 18.475 kb on - strand, within GFF2224 at 18.481 kb on - strand, within GFF2224 at 18.554 kb on + strand, within GFF2224 at 18.596 kb on + strand, within GFF2224 at 18.657 kb on + strand, within GFF2224 at 18.657 kb on + strand, within GFF2224 at 18.710 kb on + strand, within GFF2224 at 18.713 kb on - strand, within GFF2224 at 18.761 kb on - strand, within GFF2224 at 18.763 kb on + strand, within GFF2224 at 18.906 kb on + strand at 18.991 kb on - strand at 19.500 kb on - strand, within GFF2225 at 19.560 kb on + strand, within GFF2225 at 19.561 kb on - strand, within GFF2225 at 19.563 kb on + strand, within GFF2225 at 19.594 kb on + strand, within GFF2225 at 19.595 kb on - strand, within GFF2225 at 19.705 kb on - strand at 19.716 kb on + strand at 19.716 kb on + strand at 19.717 kb on - strand at 19.729 kb on + strand at 19.729 kb on + strand at 19.730 kb on - strand at 19.898 kb on + strand at 19.899 kb on - strand at 20.141 kb on + strand, within GFF2226 at 20.304 kb on - strand, within GFF2226 at 20.307 kb on - strand, within GFF2226 at 20.311 kb on - strand, within GFF2226 at 20.311 kb on - strand, within GFF2226 at 20.311 kb on - strand, within GFF2226 at 20.338 kb on - strand, within GFF2226 at 20.526 kb on + strand, within GFF2226 at 20.527 kb on - strand, within GFF2226 at 20.527 kb on - strand, within GFF2226 at 20.531 kb on - strand, within GFF2226 at 20.562 kb on + strand, within GFF2226 at 20.716 kb on + strand, within GFF2226
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 17,911 + GFF2223 0.88 +0.9 18,129 + +0.3 18,242 + -0.1 18,242 + +0.5 18,341 + GFF2224 0.21 +0.6 18,397 + GFF2224 0.29 -0.3 18,398 - GFF2224 0.29 -0.1 18,422 + GFF2224 0.32 +0.4 18,423 - GFF2224 0.32 -0.6 18,423 - GFF2224 0.32 -0.4 18,467 + GFF2224 0.38 +0.2 18,475 - GFF2224 0.39 +0.2 18,481 - GFF2224 0.40 +0.3 18,554 + GFF2224 0.50 +0.7 18,596 + GFF2224 0.56 +0.2 18,657 + GFF2224 0.64 -0.2 18,657 + GFF2224 0.64 -0.1 18,710 + GFF2224 0.71 -0.7 18,713 - GFF2224 0.72 +0.6 18,761 - GFF2224 0.78 +0.4 18,763 + GFF2224 0.79 -0.0 18,906 + -0.9 18,991 - -0.2 19,500 - GFF2225 0.68 -0.2 19,560 + GFF2225 0.75 -1.5 19,561 - GFF2225 0.75 -0.4 19,563 + GFF2225 0.75 -0.6 19,594 + GFF2225 0.78 +1.7 19,595 - GFF2225 0.78 -1.9 19,705 - -0.1 19,716 + +1.1 19,716 + -0.5 19,717 - -0.3 19,729 + +0.4 19,729 + -0.5 19,730 - -0.7 19,898 + -0.5 19,899 - -0.5 20,141 + GFF2226 0.29 -1.5 20,304 - GFF2226 0.42 -0.3 20,307 - GFF2226 0.42 -0.9 20,311 - GFF2226 0.43 +0.1 20,311 - GFF2226 0.43 -0.5 20,311 - GFF2226 0.43 -0.4 20,338 - GFF2226 0.45 -0.5 20,526 + GFF2226 0.60 -0.4 20,527 - GFF2226 0.60 -0.6 20,527 - GFF2226 0.60 +0.9 20,531 - GFF2226 0.60 +0.5 20,562 + GFF2226 0.63 -0.6 20,716 + GFF2226 0.75 -0.4
Or see this region's nucleotide sequence