Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2149 and GFF2150 are separated by 119 nucleotides GFF2150 and GFF2151 overlap by 14 nucleotides GFF2151 and GFF2152 are separated by 210 nucleotides
GFF2149 - Malate dehydrogenase (EC 1.1.1.37), at 39,305 to 40,243
GFF2149
GFF2150 - Transcriptional regulator, GntR family, at 40,363 to 40,992
GFF2150
GFF2151 - Transcriptional regulator, GntR family, at 40,979 to 41,644
GFF2151
GFF2152 - Putative membrane protein, at 41,855 to 43,123
GFF2152
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1 at 40.062 kb on - strand, within GFF2149 at 40.082 kb on + strand, within GFF2149 at 40.082 kb on + strand, within GFF2149 at 40.083 kb on - strand, within GFF2149 at 40.139 kb on - strand, within GFF2149 at 40.237 kb on + strand at 40.237 kb on + strand at 40.241 kb on + strand at 40.315 kb on + strand at 40.315 kb on + strand at 40.402 kb on - strand at 40.518 kb on - strand, within GFF2150 at 40.544 kb on + strand, within GFF2150 at 40.683 kb on - strand, within GFF2150 at 40.753 kb on + strand, within GFF2150 at 40.804 kb on + strand, within GFF2150 at 40.804 kb on + strand, within GFF2150 at 40.813 kb on + strand, within GFF2150 at 40.814 kb on - strand, within GFF2150 at 40.819 kb on - strand, within GFF2150 at 40.917 kb on + strand, within GFF2150 at 40.963 kb on - strand at 40.963 kb on - strand at 41.047 kb on + strand, within GFF2151 at 41.070 kb on + strand, within GFF2151 at 41.090 kb on - strand, within GFF2151 at 41.094 kb on + strand, within GFF2151 at 41.095 kb on - strand, within GFF2151 at 41.327 kb on + strand, within GFF2151 at 41.327 kb on + strand, within GFF2151 at 41.328 kb on - strand, within GFF2151 at 41.447 kb on - strand, within GFF2151 at 41.450 kb on - strand, within GFF2151 at 41.450 kb on - strand, within GFF2151 at 41.622 kb on - strand at 41.696 kb on - strand at 41.698 kb on + strand at 41.719 kb on + strand at 41.737 kb on - strand at 41.737 kb on - strand at 41.854 kb on + strand at 41.965 kb on + strand at 42.016 kb on + strand, within GFF2152 at 42.044 kb on - strand, within GFF2152 at 42.143 kb on - strand, within GFF2152 at 42.143 kb on - strand, within GFF2152 at 42.419 kb on + strand, within GFF2152 at 42.430 kb on + strand, within GFF2152 at 42.500 kb on + strand, within GFF2152 at 42.500 kb on + strand, within GFF2152 at 42.500 kb on + strand, within GFF2152 at 42.501 kb on - strand, within GFF2152 at 42.613 kb on + strand, within GFF2152 at 42.637 kb on - strand, within GFF2152
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 40,062 - GFF2149 0.81 +0.7 40,082 + GFF2149 0.83 -0.1 40,082 + GFF2149 0.83 -0.6 40,083 - GFF2149 0.83 +1.2 40,139 - GFF2149 0.89 +0.5 40,237 + -1.4 40,237 + +0.0 40,241 + -0.4 40,315 + -0.3 40,315 + +0.4 40,402 - -0.9 40,518 - GFF2150 0.25 -0.4 40,544 + GFF2150 0.29 +0.4 40,683 - GFF2150 0.51 -0.0 40,753 + GFF2150 0.62 +0.0 40,804 + GFF2150 0.70 -0.2 40,804 + GFF2150 0.70 -0.6 40,813 + GFF2150 0.71 -0.1 40,814 - GFF2150 0.72 +0.3 40,819 - GFF2150 0.72 -0.0 40,917 + GFF2150 0.88 -0.2 40,963 - +0.0 40,963 - +0.4 41,047 + GFF2151 0.10 -0.4 41,070 + GFF2151 0.14 -0.1 41,090 - GFF2151 0.17 -1.0 41,094 + GFF2151 0.17 -1.1 41,095 - GFF2151 0.17 +0.3 41,327 + GFF2151 0.52 +0.2 41,327 + GFF2151 0.52 +1.0 41,328 - GFF2151 0.52 -0.4 41,447 - GFF2151 0.70 -0.8 41,450 - GFF2151 0.71 -0.0 41,450 - GFF2151 0.71 -0.4 41,622 - +0.1 41,696 - -0.1 41,698 + +0.2 41,719 + +0.3 41,737 - +0.2 41,737 - +0.3 41,854 + +0.2 41,965 + -0.4 42,016 + GFF2152 0.13 +0.5 42,044 - GFF2152 0.15 +0.3 42,143 - GFF2152 0.23 +0.3 42,143 - GFF2152 0.23 +1.0 42,419 + GFF2152 0.44 -0.5 42,430 + GFF2152 0.45 -0.4 42,500 + GFF2152 0.51 -0.5 42,500 + GFF2152 0.51 +0.4 42,500 + GFF2152 0.51 -1.3 42,501 - GFF2152 0.51 +0.2 42,613 + GFF2152 0.60 +0.1 42,637 - GFF2152 0.62 -2.6
Or see this region's nucleotide sequence