Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF19 and GFF20 are separated by 70 nucleotides GFF20 and GFF21 are separated by 189 nucleotides GFF21 and GFF22 are separated by 7 nucleotides GFF22 and GFF23 are separated by 38 nucleotides
GFF19 - hypothetical protein, at 86 to 208
GFF19
GFF20 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-), at 279 to 845
GFF20
GFF21 - Methionine ABC transporter ATP-binding protein, at 1,035 to 2,066
GFF21
GFF22 - Methionine ABC transporter permease protein, at 2,074 to 2,712
GFF22
GFF23 - Methionine ABC transporter substrate-binding protein, at 2,751 to 3,566
GFF23
Position (kb) 1
2
3 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 0.206 kb on + strand at 0.425 kb on + strand, within GFF20 at 0.426 kb on - strand, within GFF20 at 0.450 kb on - strand, within GFF20 at 0.451 kb on + strand, within GFF20 at 0.452 kb on - strand, within GFF20 at 0.547 kb on - strand, within GFF20 at 0.548 kb on + strand, within GFF20 at 0.577 kb on + strand, within GFF20 at 0.578 kb on - strand, within GFF20 at 0.751 kb on - strand, within GFF20 at 0.782 kb on - strand, within GFF20 at 0.918 kb on - strand at 1.100 kb on + strand at 1.193 kb on - strand, within GFF21 at 1.240 kb on + strand, within GFF21 at 1.506 kb on - strand, within GFF21 at 1.622 kb on + strand, within GFF21 at 1.652 kb on + strand, within GFF21 at 1.653 kb on - strand, within GFF21 at 1.691 kb on + strand, within GFF21 at 1.691 kb on + strand, within GFF21 at 1.920 kb on - strand, within GFF21 at 1.922 kb on + strand, within GFF21 at 1.923 kb on - strand, within GFF21 at 1.968 kb on - strand at 2.044 kb on + strand at 2.050 kb on + strand at 2.173 kb on + strand, within GFF22 at 2.174 kb on - strand, within GFF22 at 2.197 kb on - strand, within GFF22 at 2.263 kb on - strand, within GFF22 at 2.339 kb on + strand, within GFF22 at 2.376 kb on + strand, within GFF22 at 2.396 kb on + strand, within GFF22 at 2.515 kb on - strand, within GFF22 at 2.594 kb on - strand, within GFF22 at 2.609 kb on - strand, within GFF22 at 2.609 kb on - strand, within GFF22 at 2.628 kb on + strand, within GFF22 at 2.707 kb on - strand at 2.731 kb on + strand at 2.849 kb on + strand, within GFF23 at 2.949 kb on - strand, within GFF23
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 206 + -0.5 425 + GFF20 0.26 +3.0 426 - GFF20 0.26 +2.9 450 - GFF20 0.30 +2.9 451 + GFF20 0.30 +3.4 452 - GFF20 0.31 +2.1 547 - GFF20 0.47 +2.9 548 + GFF20 0.47 +2.5 577 + GFF20 0.53 +3.1 578 - GFF20 0.53 +2.8 751 - GFF20 0.83 +3.3 782 - GFF20 0.89 +3.5 918 - -0.6 1,100 + +0.3 1,193 - GFF21 0.15 -0.5 1,240 + GFF21 0.20 -0.2 1,506 - GFF21 0.46 +0.1 1,622 + GFF21 0.57 -1.2 1,652 + GFF21 0.60 +0.3 1,653 - GFF21 0.60 -0.3 1,691 + GFF21 0.64 -2.0 1,691 + GFF21 0.64 -1.0 1,920 - GFF21 0.86 -0.2 1,922 + GFF21 0.86 -0.7 1,923 - GFF21 0.86 -0.3 1,968 - +0.3 2,044 + -0.3 2,050 + -0.9 2,173 + GFF22 0.15 -0.2 2,174 - GFF22 0.16 +0.2 2,197 - GFF22 0.19 +0.1 2,263 - GFF22 0.30 +0.4 2,339 + GFF22 0.41 -0.7 2,376 + GFF22 0.47 -0.6 2,396 + GFF22 0.50 -0.2 2,515 - GFF22 0.69 +0.5 2,594 - GFF22 0.81 +0.1 2,609 - GFF22 0.84 +0.1 2,609 - GFF22 0.84 +0.5 2,628 + GFF22 0.87 +0.1 2,707 - +0.1 2,731 + -0.2 2,849 + GFF23 0.12 +1.3 2,949 - GFF23 0.24 +1.1
Or see this region's nucleotide sequence