Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1968 and GFF1969 are separated by 114 nucleotides GFF1969 and GFF1970 are separated by 286 nucleotides GFF1970 and GFF1971 are separated by 341 nucleotides GFF1971 and GFF1972 are separated by 232 nucleotides
GFF1968 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 160,951 to 161,871
GFF1968
GFF1969 - hypothetical protein, at 161,986 to 162,108
GFF1969
GFF1970 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 162,395 to 163,633
GFF1970
GFF1971 - hypothetical protein, at 163,975 to 164,121
GFF1971
GFF1972 - Glucokinase (EC 2.7.1.2), at 164,354 to 165,319
GFF1972
Position (kb)
162
163
164 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 161.627 kb on - strand, within GFF1968 at 161.630 kb on + strand, within GFF1968 at 161.707 kb on + strand, within GFF1968 at 161.708 kb on - strand, within GFF1968 at 161.710 kb on - strand, within GFF1968 at 161.710 kb on - strand, within GFF1968 at 161.917 kb on + strand at 162.113 kb on - strand at 162.216 kb on - strand at 162.225 kb on + strand at 162.248 kb on - strand at 162.343 kb on + strand at 162.519 kb on - strand, within GFF1970 at 162.519 kb on - strand, within GFF1970 at 162.519 kb on - strand, within GFF1970 at 162.621 kb on + strand, within GFF1970 at 162.778 kb on + strand, within GFF1970 at 162.811 kb on - strand, within GFF1970 at 162.827 kb on - strand, within GFF1970 at 162.892 kb on + strand, within GFF1970 at 162.895 kb on + strand, within GFF1970 at 162.897 kb on + strand, within GFF1970 at 163.027 kb on - strand, within GFF1970 at 163.225 kb on - strand, within GFF1970 at 163.228 kb on + strand, within GFF1970 at 163.229 kb on - strand, within GFF1970 at 163.229 kb on - strand, within GFF1970 at 163.229 kb on - strand, within GFF1970 at 163.363 kb on - strand, within GFF1970 at 163.363 kb on - strand, within GFF1970 at 163.363 kb on - strand, within GFF1970 at 163.375 kb on - strand, within GFF1970 at 163.523 kb on - strand at 163.571 kb on - strand at 163.983 kb on + strand at 163.983 kb on + strand at 164.081 kb on - strand, within GFF1971 at 164.083 kb on - strand, within GFF1971 at 164.358 kb on - strand at 164.369 kb on + strand at 164.369 kb on + strand at 164.414 kb on - strand at 164.452 kb on - strand, within GFF1972 at 164.452 kb on - strand, within GFF1972 at 164.542 kb on + strand, within GFF1972 at 164.544 kb on + strand, within GFF1972 at 164.544 kb on + strand, within GFF1972
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 161,627 - GFF1968 0.73 +0.4 161,630 + GFF1968 0.74 +0.7 161,707 + GFF1968 0.82 +0.0 161,708 - GFF1968 0.82 -0.3 161,710 - GFF1968 0.82 +0.1 161,710 - GFF1968 0.82 -0.2 161,917 + +0.2 162,113 - +0.9 162,216 - +0.7 162,225 + +0.5 162,248 - +1.1 162,343 + +0.1 162,519 - GFF1970 0.10 -0.0 162,519 - GFF1970 0.10 -0.3 162,519 - GFF1970 0.10 +0.1 162,621 + GFF1970 0.18 -0.0 162,778 + GFF1970 0.31 +0.3 162,811 - GFF1970 0.34 +0.9 162,827 - GFF1970 0.35 +0.3 162,892 + GFF1970 0.40 +1.8 162,895 + GFF1970 0.40 +0.3 162,897 + GFF1970 0.41 +2.3 163,027 - GFF1970 0.51 +0.0 163,225 - GFF1970 0.67 +1.1 163,228 + GFF1970 0.67 +0.7 163,229 - GFF1970 0.67 -0.6 163,229 - GFF1970 0.67 +0.7 163,229 - GFF1970 0.67 +0.5 163,363 - GFF1970 0.78 +1.3 163,363 - GFF1970 0.78 -0.1 163,363 - GFF1970 0.78 +0.3 163,375 - GFF1970 0.79 -0.6 163,523 - -0.4 163,571 - -0.1 163,983 + -0.4 163,983 + -0.5 164,081 - GFF1971 0.72 +1.2 164,083 - GFF1971 0.73 +0.1 164,358 - +0.3 164,369 + -2.0 164,369 + -0.2 164,414 - +0.4 164,452 - GFF1972 0.10 -0.6 164,452 - GFF1972 0.10 +0.0 164,542 + GFF1972 0.19 +0.4 164,544 + GFF1972 0.20 +0.7 164,544 + GFF1972 0.20 +0.7
Or see this region's nucleotide sequence