Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1867

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1866 and GFF1867 overlap by 4 nucleotidesGFF1867 and GFF1868 overlap by 1 nucleotidesGFF1868 and GFF1869 are separated by 14 nucleotides GFF1866 - O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26), at 58,066 to 59,433 GFF1866 GFF1867 - O-succinylbenzoate synthase (EC 4.2.1.113), at 59,430 to 60,392 GFF1867 GFF1868 - Naphthoate synthase (EC 4.1.3.36), at 60,392 to 61,249 GFF1868 GFF1869 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1- carboxylate synthase (EC 4.2.99.20), at 61,264 to 62,022 GFF1869 Position (kb) 59 60 61Strain fitness (log2 ratio) -2 -1 0 1at 58.606 kb on - strand, within GFF1866at 58.779 kb on + strand, within GFF1866at 58.780 kb on - strand, within GFF1866at 58.857 kb on + strand, within GFF1866at 58.858 kb on - strand, within GFF1866at 58.858 kb on - strand, within GFF1866at 58.858 kb on - strand, within GFF1866at 58.860 kb on + strand, within GFF1866at 58.861 kb on - strand, within GFF1866at 58.861 kb on - strand, within GFF1866at 58.971 kb on + strand, within GFF1866at 58.971 kb on + strand, within GFF1866at 58.971 kb on + strand, within GFF1866at 58.972 kb on - strand, within GFF1866at 58.972 kb on - strand, within GFF1866at 58.972 kb on - strand, within GFF1866at 58.975 kb on + strand, within GFF1866at 58.976 kb on - strand, within GFF1866at 58.976 kb on - strand, within GFF1866at 58.976 kb on - strand, within GFF1866at 58.976 kb on - strand, within GFF1866at 59.075 kb on - strand, within GFF1866at 59.288 kb on - strand, within GFF1866at 59.523 kb on - strandat 59.664 kb on - strand, within GFF1867at 59.685 kb on - strand, within GFF1867at 59.864 kb on - strand, within GFF1867at 59.864 kb on - strand, within GFF1867at 60.202 kb on - strand, within GFF1867at 60.202 kb on - strand, within GFF1867at 60.202 kb on - strand, within GFF1867at 60.302 kb on - strandat 60.376 kb on - strandat 60.681 kb on + strand, within GFF1868at 60.739 kb on + strand, within GFF1868at 60.742 kb on - strand, within GFF1868at 61.034 kb on - strand, within GFF1868

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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58,606 - GFF1866 0.39 +0.2
58,779 + GFF1866 0.52 -1.6
58,780 - GFF1866 0.52 -0.6
58,857 + GFF1866 0.58 -0.0
58,858 - GFF1866 0.58 +1.4
58,858 - GFF1866 0.58 -2.6
58,858 - GFF1866 0.58 +1.0
58,860 + GFF1866 0.58 -0.6
58,861 - GFF1866 0.58 +0.0
58,861 - GFF1866 0.58 -0.8
58,971 + GFF1866 0.66 +0.2
58,971 + GFF1866 0.66 -1.6
58,971 + GFF1866 0.66 +0.0
58,972 - GFF1866 0.66 -0.0
58,972 - GFF1866 0.66 -1.0
58,972 - GFF1866 0.66 -0.2
58,975 + GFF1866 0.66 +1.0
58,976 - GFF1866 0.67 -0.5
58,976 - GFF1866 0.67 -0.2
58,976 - GFF1866 0.67 -0.4
58,976 - GFF1866 0.67 +0.2
59,075 - GFF1866 0.74 -0.2
59,288 - GFF1866 0.89 +0.1
59,523 - -0.0
59,664 - GFF1867 0.24 +0.5
59,685 - GFF1867 0.26 -1.2
59,864 - GFF1867 0.45 -0.5
59,864 - GFF1867 0.45 +0.2
60,202 - GFF1867 0.80 +0.6
60,202 - GFF1867 0.80 -0.6
60,202 - GFF1867 0.80 +0.1
60,302 - -0.5
60,376 - +0.2
60,681 + GFF1868 0.34 +0.6
60,739 + GFF1868 0.40 +0.7
60,742 - GFF1868 0.41 -0.1
61,034 - GFF1868 0.75 +0.8

Or see this region's nucleotide sequence