Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1477 and GFF1478 are separated by 95 nucleotides GFF1478 and GFF1479 are separated by 146 nucleotides GFF1479 and GFF1480 are separated by 409 nucleotides
GFF1477 - Fumarate hydratase class I, anaerobic (EC 4.2.1.2), at 145,496 to 147,142
GFF1477
GFF1478 - C4-dicarboxylate transporter DcuB, at 147,238 to 148,578
GFF1478
GFF1479 - hypothetical protein, at 148,725 to 148,898
GFF1479
GFF1480 - Fumarate respiration transcriptional regulator DcuR, at 149,308 to 150,027
GFF1480
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-1
0
1 at 146.243 kb on + strand, within GFF1477 at 146.386 kb on - strand, within GFF1477 at 146.464 kb on - strand, within GFF1477 at 146.557 kb on - strand, within GFF1477 at 146.557 kb on - strand, within GFF1477 at 146.562 kb on + strand, within GFF1477 at 146.610 kb on + strand, within GFF1477 at 146.679 kb on + strand, within GFF1477 at 146.679 kb on + strand, within GFF1477 at 146.683 kb on + strand, within GFF1477 at 146.684 kb on - strand, within GFF1477 at 146.727 kb on + strand, within GFF1477 at 146.727 kb on + strand, within GFF1477 at 146.728 kb on - strand, within GFF1477 at 146.728 kb on - strand, within GFF1477 at 146.731 kb on + strand, within GFF1477 at 146.732 kb on - strand, within GFF1477 at 146.889 kb on + strand, within GFF1477 at 146.889 kb on + strand, within GFF1477 at 147.028 kb on + strand at 147.079 kb on - strand at 147.081 kb on + strand at 147.158 kb on + strand at 147.408 kb on + strand, within GFF1478 at 147.410 kb on + strand, within GFF1478 at 147.410 kb on + strand, within GFF1478 at 147.410 kb on + strand, within GFF1478 at 147.411 kb on - strand, within GFF1478 at 147.411 kb on - strand, within GFF1478 at 147.411 kb on - strand, within GFF1478 at 147.414 kb on - strand, within GFF1478 at 147.414 kb on - strand, within GFF1478 at 147.805 kb on + strand, within GFF1478 at 147.806 kb on - strand, within GFF1478 at 147.926 kb on + strand, within GFF1478 at 148.002 kb on + strand, within GFF1478 at 148.207 kb on - strand, within GFF1478 at 148.309 kb on - strand, within GFF1478 at 148.309 kb on - strand, within GFF1478 at 148.309 kb on - strand, within GFF1478 at 148.316 kb on + strand, within GFF1478 at 148.317 kb on - strand, within GFF1478 at 148.427 kb on - strand, within GFF1478 at 148.544 kb on - strand at 148.937 kb on + strand at 149.318 kb on + strand at 149.527 kb on + strand, within GFF1480 at 149.527 kb on + strand, within GFF1480
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 146,243 + GFF1477 0.45 +0.5 146,386 - GFF1477 0.54 -0.6 146,464 - GFF1477 0.59 -0.5 146,557 - GFF1477 0.64 -0.9 146,557 - GFF1477 0.64 -0.5 146,562 + GFF1477 0.65 +0.1 146,610 + GFF1477 0.68 -0.2 146,679 + GFF1477 0.72 +0.3 146,679 + GFF1477 0.72 -0.4 146,683 + GFF1477 0.72 -0.4 146,684 - GFF1477 0.72 -0.1 146,727 + GFF1477 0.75 -0.2 146,727 + GFF1477 0.75 +0.1 146,728 - GFF1477 0.75 -0.1 146,728 - GFF1477 0.75 -0.1 146,731 + GFF1477 0.75 -0.1 146,732 - GFF1477 0.75 +0.6 146,889 + GFF1477 0.85 -0.3 146,889 + GFF1477 0.85 -0.1 147,028 + -0.2 147,079 - -0.8 147,081 + -0.1 147,158 + +0.3 147,408 + GFF1478 0.13 -0.3 147,410 + GFF1478 0.13 -1.3 147,410 + GFF1478 0.13 -0.0 147,410 + GFF1478 0.13 +1.1 147,411 - GFF1478 0.13 -0.1 147,411 - GFF1478 0.13 +0.3 147,411 - GFF1478 0.13 -0.1 147,414 - GFF1478 0.13 -0.5 147,414 - GFF1478 0.13 -0.1 147,805 + GFF1478 0.42 +0.1 147,806 - GFF1478 0.42 -0.6 147,926 + GFF1478 0.51 -0.1 148,002 + GFF1478 0.57 +0.2 148,207 - GFF1478 0.72 -0.1 148,309 - GFF1478 0.80 -0.6 148,309 - GFF1478 0.80 -0.4 148,309 - GFF1478 0.80 -1.7 148,316 + GFF1478 0.80 -1.3 148,317 - GFF1478 0.80 +0.1 148,427 - GFF1478 0.89 +0.2 148,544 - -0.0 148,937 + -0.7 149,318 + -0.1 149,527 + GFF1480 0.30 +0.8 149,527 + GFF1480 0.30 -1.3
Or see this region's nucleotide sequence