Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1465 and GFF1466 are separated by 119 nucleotides GFF1466 and GFF1467 are separated by 467 nucleotides GFF1467 and GFF1468 are separated by 166 nucleotides
GFF1465 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 129,735 to 130,178
GFF1465
GFF1466 - Alkylphosphonate utilization operon protein PhnA, at 130,298 to 130,633
GFF1466
GFF1467 - L-Proline/Glycine betaine transporter ProP, at 131,101 to 132,603
GFF1467
GFF1468 - Sensor protein basS/pmrB (EC 2.7.3.-), at 132,770 to 133,849
GFF1468
Position (kb) 131
132
133 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 130.121 kb on - strand, within GFF1465 at 130.123 kb on + strand, within GFF1465 at 130.185 kb on + strand at 130.208 kb on + strand at 130.367 kb on - strand, within GFF1466 at 130.430 kb on - strand, within GFF1466 at 130.590 kb on + strand, within GFF1466 at 130.591 kb on - strand, within GFF1466 at 130.825 kb on + strand at 130.825 kb on + strand at 130.840 kb on + strand at 130.841 kb on - strand at 130.863 kb on + strand at 130.863 kb on + strand at 130.863 kb on + strand at 130.864 kb on - strand at 130.864 kb on - strand at 130.864 kb on - strand at 130.865 kb on + strand at 130.865 kb on + strand at 130.865 kb on + strand at 130.866 kb on - strand at 130.866 kb on - strand at 130.916 kb on + strand at 130.950 kb on + strand at 130.950 kb on + strand at 130.950 kb on + strand at 130.972 kb on + strand at 130.972 kb on + strand at 130.972 kb on + strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.993 kb on + strand at 131.003 kb on - strand at 131.047 kb on + strand at 131.065 kb on - strand at 131.066 kb on + strand at 131.066 kb on + strand at 131.067 kb on - strand at 131.069 kb on - strand at 131.131 kb on - strand at 131.163 kb on + strand at 131.257 kb on - strand, within GFF1467 at 131.377 kb on + strand, within GFF1467 at 131.378 kb on - strand, within GFF1467 at 131.416 kb on - strand, within GFF1467 at 131.736 kb on + strand, within GFF1467 at 131.746 kb on + strand, within GFF1467 at 131.747 kb on - strand, within GFF1467 at 131.926 kb on + strand, within GFF1467 at 131.926 kb on + strand, within GFF1467 at 131.927 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.953 kb on + strand, within GFF1467 at 131.954 kb on - strand, within GFF1467 at 131.964 kb on + strand, within GFF1467 at 131.964 kb on + strand, within GFF1467 at 131.965 kb on - strand, within GFF1467 at 131.974 kb on + strand, within GFF1467 at 132.145 kb on - strand, within GFF1467 at 132.205 kb on - strand, within GFF1467 at 132.406 kb on - strand, within GFF1467 at 132.507 kb on + strand at 132.508 kb on - strand at 132.641 kb on + strand at 132.683 kb on - strand at 132.711 kb on + strand at 132.711 kb on + strand at 132.739 kb on + strand at 132.740 kb on - strand at 132.757 kb on + strand at 132.761 kb on - strand at 132.771 kb on + strand at 132.848 kb on + strand at 132.848 kb on + strand at 132.930 kb on - strand, within GFF1468 at 132.930 kb on - strand, within GFF1468 at 133.038 kb on + strand, within GFF1468 at 133.038 kb on + strand, within GFF1468 at 133.039 kb on - strand, within GFF1468 at 133.083 kb on - strand, within GFF1468 at 133.083 kb on - strand, within GFF1468 at 133.176 kb on + strand, within GFF1468 at 133.176 kb on + strand, within GFF1468 at 133.177 kb on - strand, within GFF1468 at 133.219 kb on - strand, within GFF1468 at 133.340 kb on + strand, within GFF1468 at 133.379 kb on + strand, within GFF1468 at 133.517 kb on - strand, within GFF1468
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 130,121 - GFF1465 0.87 -0.2 130,123 + GFF1465 0.87 -0.4 130,185 + -0.2 130,208 + +0.1 130,367 - GFF1466 0.21 -0.9 130,430 - GFF1466 0.39 -0.1 130,590 + GFF1466 0.87 -0.6 130,591 - GFF1466 0.87 -0.8 130,825 + -0.4 130,825 + -1.2 130,840 + -0.5 130,841 - +0.2 130,863 + -0.0 130,863 + +0.2 130,863 + +0.1 130,864 - +1.0 130,864 - -0.9 130,864 - -1.0 130,865 + -1.6 130,865 + +0.1 130,865 + -0.4 130,866 - -0.2 130,866 - -0.4 130,916 + +0.2 130,950 + -0.4 130,950 + -0.3 130,950 + -0.3 130,972 + -0.7 130,972 + -0.2 130,972 + +0.2 130,973 - -0.5 130,973 - -0.5 130,973 - +0.2 130,973 - +0.2 130,973 - -0.4 130,973 - -0.0 130,993 + -0.3 131,003 - +0.3 131,047 + -0.0 131,065 - -0.0 131,066 + -0.8 131,066 + +0.4 131,067 - -0.5 131,069 - -0.0 131,131 - -0.9 131,163 + +0.2 131,257 - GFF1467 0.10 +1.2 131,377 + GFF1467 0.18 -0.7 131,378 - GFF1467 0.18 -0.8 131,416 - GFF1467 0.21 -0.7 131,736 + GFF1467 0.42 -0.1 131,746 + GFF1467 0.43 -0.9 131,747 - GFF1467 0.43 +0.1 131,926 + GFF1467 0.55 +0.5 131,926 + GFF1467 0.55 -0.6 131,927 - GFF1467 0.55 -0.0 131,942 - GFF1467 0.56 +0.1 131,942 - GFF1467 0.56 +0.2 131,942 - GFF1467 0.56 -0.9 131,942 - GFF1467 0.56 -0.0 131,953 + GFF1467 0.57 +0.0 131,954 - GFF1467 0.57 +0.1 131,964 + GFF1467 0.57 +0.2 131,964 + GFF1467 0.57 +3.1 131,965 - GFF1467 0.57 -2.3 131,974 + GFF1467 0.58 -0.5 132,145 - GFF1467 0.69 +1.4 132,205 - GFF1467 0.73 +0.0 132,406 - GFF1467 0.87 -1.3 132,507 + +0.8 132,508 - -0.5 132,641 + -0.6 132,683 - -0.4 132,711 + +0.0 132,711 + -0.4 132,739 + +0.2 132,740 - +0.2 132,757 + +0.6 132,761 - -0.7 132,771 + +0.6 132,848 + +0.3 132,848 + -0.4 132,930 - GFF1468 0.15 +0.6 132,930 - GFF1468 0.15 +1.1 133,038 + GFF1468 0.25 +0.2 133,038 + GFF1468 0.25 -0.4 133,039 - GFF1468 0.25 +0.1 133,083 - GFF1468 0.29 -0.6 133,083 - GFF1468 0.29 +0.7 133,176 + GFF1468 0.38 +0.2 133,176 + GFF1468 0.38 -0.3 133,177 - GFF1468 0.38 -0.7 133,219 - GFF1468 0.42 +1.9 133,340 + GFF1468 0.53 -1.0 133,379 + GFF1468 0.56 +0.2 133,517 - GFF1468 0.69 +0.2
Or see this region's nucleotide sequence