Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1426

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1424 and GFF1425 are separated by 128 nucleotidesGFF1425 and GFF1426 are separated by 2 nucleotidesGFF1426 and GFF1427 are separated by 0 nucleotidesGFF1427 and GFF1428 overlap by 14 nucleotidesGFF1428 and GFF1429 are separated by 143 nucleotides GFF1424 - NMN phosphatase (EC 3.1.3.5); Class B acid phosphatase precursor (EC 3.1.3.2), at 70,814 to 71,527 GFF1424 GFF1425 - FIG004064: hypothetical protein, at 71,656 to 72,072 GFF1425 GFF1426 - Protein yjbR, at 72,075 to 72,431 GFF1426 GFF1427 - Putative inner membrane protein, at 72,432 to 72,770 GFF1427 GFF1428 - Putative outer membrane lipoprotein, at 72,757 to 73,200 GFF1428 GFF1429 - Excinuclease ABC subunit A, at 73,344 to 76,169 GFF1429 Position (kb) 72 73Strain fitness (log2 ratio) -1 0 1at 71.097 kb on - strand, within GFF1424at 71.214 kb on - strand, within GFF1424at 71.214 kb on - strand, within GFF1424at 71.214 kb on - strand, within GFF1424at 71.401 kb on + strand, within GFF1424at 71.402 kb on - strand, within GFF1424at 71.620 kb on - strandat 71.655 kb on + strandat 71.656 kb on - strandat 71.656 kb on - strandat 71.656 kb on - strandat 71.662 kb on + strandat 71.676 kb on + strandat 71.676 kb on + strandat 71.783 kb on + strand, within GFF1425at 71.788 kb on - strand, within GFF1425at 71.896 kb on + strand, within GFF1425at 71.896 kb on + strand, within GFF1425at 71.925 kb on + strand, within GFF1425at 71.928 kb on + strand, within GFF1425at 72.003 kb on + strand, within GFF1425at 72.003 kb on + strand, within GFF1425at 72.004 kb on - strand, within GFF1425at 72.059 kb on + strandat 72.190 kb on + strand, within GFF1426at 72.190 kb on + strand, within GFF1426at 72.190 kb on + strand, within GFF1426at 72.253 kb on + strand, within GFF1426at 72.274 kb on + strand, within GFF1426at 72.373 kb on - strand, within GFF1426at 72.433 kb on + strandat 72.485 kb on + strand, within GFF1427at 72.522 kb on - strand, within GFF1427at 72.532 kb on - strand, within GFF1427at 72.532 kb on - strand, within GFF1427at 72.566 kb on + strand, within GFF1427at 72.674 kb on + strand, within GFF1427at 72.674 kb on + strand, within GFF1427at 72.674 kb on + strand, within GFF1427at 72.675 kb on - strand, within GFF1427at 72.676 kb on + strand, within GFF1427at 72.676 kb on + strand, within GFF1427at 72.745 kb on + strandat 72.750 kb on + strandat 72.750 kb on + strandat 72.751 kb on - strandat 72.758 kb on + strandat 72.820 kb on + strand, within GFF1428at 72.875 kb on + strand, within GFF1428at 72.875 kb on + strand, within GFF1428at 72.875 kb on + strand, within GFF1428at 72.875 kb on + strand, within GFF1428at 72.876 kb on - strand, within GFF1428at 72.876 kb on - strand, within GFF1428at 72.879 kb on + strand, within GFF1428at 72.923 kb on + strand, within GFF1428at 73.100 kb on - strand, within GFF1428at 73.105 kb on + strand, within GFF1428at 73.105 kb on + strand, within GFF1428at 73.105 kb on + strand, within GFF1428at 73.106 kb on - strand, within GFF1428at 73.361 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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71,097 - GFF1424 0.40 +0.0
71,214 - GFF1424 0.56 +0.2
71,214 - GFF1424 0.56 +0.0
71,214 - GFF1424 0.56 -0.5
71,401 + GFF1424 0.82 +0.2
71,402 - GFF1424 0.82 +0.4
71,620 - -0.5
71,655 + +1.0
71,656 - +0.2
71,656 - -0.2
71,656 - -0.0
71,662 + -0.4
71,676 + +0.1
71,676 + +1.3
71,783 + GFF1425 0.30 +0.3
71,788 - GFF1425 0.32 +0.4
71,896 + GFF1425 0.58 -0.7
71,896 + GFF1425 0.58 -0.6
71,925 + GFF1425 0.65 -0.9
71,928 + GFF1425 0.65 +0.2
72,003 + GFF1425 0.83 +0.7
72,003 + GFF1425 0.83 +0.4
72,004 - GFF1425 0.83 +0.0
72,059 + -0.2
72,190 + GFF1426 0.32 +0.8
72,190 + GFF1426 0.32 +0.6
72,190 + GFF1426 0.32 +0.3
72,253 + GFF1426 0.50 +0.5
72,274 + GFF1426 0.56 +0.5
72,373 - GFF1426 0.83 -0.4
72,433 + +0.6
72,485 + GFF1427 0.16 +0.4
72,522 - GFF1427 0.27 +1.6
72,532 - GFF1427 0.29 -0.3
72,532 - GFF1427 0.29 -0.0
72,566 + GFF1427 0.40 +0.5
72,674 + GFF1427 0.71 +0.5
72,674 + GFF1427 0.71 +0.2
72,674 + GFF1427 0.71 +0.9
72,675 - GFF1427 0.72 -0.1
72,676 + GFF1427 0.72 +0.3
72,676 + GFF1427 0.72 -0.1
72,745 + +0.6
72,750 + +0.0
72,750 + +0.5
72,751 - +0.7
72,758 + +0.6
72,820 + GFF1428 0.14 +1.1
72,875 + GFF1428 0.27 -0.1
72,875 + GFF1428 0.27 +0.7
72,875 + GFF1428 0.27 +0.7
72,875 + GFF1428 0.27 +0.4
72,876 - GFF1428 0.27 +0.2
72,876 - GFF1428 0.27 -0.1
72,879 + GFF1428 0.27 -0.6
72,923 + GFF1428 0.37 +0.0
73,100 - GFF1428 0.77 +0.4
73,105 + GFF1428 0.78 -0.5
73,105 + GFF1428 0.78 +0.3
73,105 + GFF1428 0.78 +0.1
73,106 - GFF1428 0.79 +0.2
73,361 + +0.4

Or see this region's nucleotide sequence