Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1292 and GFF1293 are separated by 2 nucleotides GFF1293 and GFF1294 are separated by 9 nucleotides GFF1294 and GFF1295 are separated by 108 nucleotides GFF1295 and GFF1296 are separated by 38 nucleotides
GFF1292 - Phage tail length tape-measure protein 1, at 14,933 to 18,028
GFF1292
GFF1293 - Phage minor tail protein, at 18,031 to 18,360
GFF1293
GFF1294 - Phage minor tail protein, at 18,370 to 19,068
GFF1294
GFF1295 - Phage tail assembly protein, at 19,177 to 19,812
GFF1295
GFF1296 - Phage tail assembly protein I, at 19,851 to 20,357
GFF1296
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.642 kb on + strand, within GFF1292 at 17.643 kb on - strand, within GFF1292 at 17.714 kb on + strand, within GFF1292 at 17.732 kb on - strand at 17.799 kb on + strand at 17.800 kb on - strand at 17.803 kb on - strand at 17.827 kb on + strand at 17.883 kb on - strand at 17.947 kb on - strand at 17.949 kb on - strand at 17.950 kb on + strand at 17.950 kb on + strand at 17.951 kb on - strand at 18.160 kb on + strand, within GFF1293 at 18.161 kb on - strand, within GFF1293 at 18.183 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.391 kb on - strand at 18.536 kb on - strand, within GFF1294 at 18.689 kb on + strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.807 kb on - strand, within GFF1294 at 18.888 kb on + strand, within GFF1294 at 18.896 kb on + strand, within GFF1294 at 18.919 kb on + strand, within GFF1294 at 18.921 kb on + strand, within GFF1294 at 18.957 kb on + strand, within GFF1294 at 19.054 kb on - strand at 19.089 kb on + strand at 19.166 kb on - strand at 19.182 kb on + strand at 19.204 kb on + strand at 19.409 kb on - strand, within GFF1295 at 19.433 kb on - strand, within GFF1295 at 19.453 kb on + strand, within GFF1295 at 19.454 kb on - strand, within GFF1295 at 19.555 kb on + strand, within GFF1295 at 19.700 kb on - strand, within GFF1295 at 19.700 kb on - strand, within GFF1295 at 19.811 kb on - strand at 19.894 kb on + strand at 19.894 kb on + strand at 19.901 kb on - strand at 19.977 kb on - strand, within GFF1296
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 17,642 + GFF1292 0.88 +0.1 17,643 - GFF1292 0.88 +0.6 17,714 + GFF1292 0.90 -0.8 17,732 - -0.2 17,799 + -2.3 17,800 - -1.5 17,803 - +0.0 17,827 + +0.5 17,883 - +0.0 17,947 - -0.0 17,949 - -0.1 17,950 + +0.1 17,950 + +0.2 17,951 - +0.2 18,160 + GFF1293 0.39 -0.1 18,161 - GFF1293 0.39 -0.1 18,183 + GFF1293 0.46 -0.5 18,261 + GFF1293 0.70 -0.0 18,261 + GFF1293 0.70 -0.6 18,261 + GFF1293 0.70 +0.1 18,391 - +0.6 18,536 - GFF1294 0.24 -0.7 18,689 + GFF1294 0.46 +0.5 18,693 - GFF1294 0.46 +0.0 18,693 - GFF1294 0.46 +0.4 18,693 - GFF1294 0.46 +0.1 18,694 + GFF1294 0.46 -1.0 18,694 + GFF1294 0.46 +0.8 18,694 + GFF1294 0.46 +0.6 18,695 - GFF1294 0.46 -2.0 18,695 - GFF1294 0.46 +0.3 18,695 - GFF1294 0.46 +0.7 18,807 - GFF1294 0.63 +0.7 18,888 + GFF1294 0.74 -0.9 18,896 + GFF1294 0.75 +0.4 18,919 + GFF1294 0.79 +0.7 18,921 + GFF1294 0.79 +0.3 18,957 + GFF1294 0.84 -0.2 19,054 - -0.3 19,089 + +0.0 19,166 - +0.2 19,182 + +2.2 19,204 + +0.5 19,409 - GFF1295 0.36 -0.4 19,433 - GFF1295 0.40 +0.2 19,453 + GFF1295 0.43 -1.0 19,454 - GFF1295 0.44 -0.1 19,555 + GFF1295 0.59 -0.1 19,700 - GFF1295 0.82 +0.7 19,700 - GFF1295 0.82 -0.1 19,811 - -0.1 19,894 + +0.4 19,894 + +0.5 19,901 - -0.1 19,977 - GFF1296 0.25 -0.1
Or see this region's nucleotide sequence