Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1242 and GFF1243 are separated by 188 nucleotides GFF1243 and GFF1244 overlap by 11 nucleotides GFF1244 and GFF1245 are separated by 36 nucleotides
GFF1242 - Mobile element protein, at 73,146 to 74,183
GFF1242
GFF1243 - COG3267: Type II secretory pathway, component ExeA (predicted ATPase), at 74,372 to 75,340
GFF1243
GFF1244 - Putative integrase protein, at 75,330 to 75,881
GFF1244
GFF1245 - FIG01046087: hypothetical protein, at 75,918 to 76,556
GFF1245
Position (kb)
74
75
76 Strain fitness (log2 ratio)
-2
-1
0
1 at 73.407 kb on - strand, within GFF1242 at 73.432 kb on + strand, within GFF1242 at 73.548 kb on + strand, within GFF1242 at 73.565 kb on + strand, within GFF1242 at 73.614 kb on + strand, within GFF1242 at 73.894 kb on - strand, within GFF1242 at 73.929 kb on - strand, within GFF1242 at 73.932 kb on + strand, within GFF1242 at 73.933 kb on - strand, within GFF1242 at 73.933 kb on - strand, within GFF1242 at 73.933 kb on - strand, within GFF1242 at 73.944 kb on - strand, within GFF1242 at 73.944 kb on - strand, within GFF1242 at 73.944 kb on - strand, within GFF1242 at 74.011 kb on - strand, within GFF1242 at 74.038 kb on + strand, within GFF1242 at 74.123 kb on + strand at 74.195 kb on - strand at 74.195 kb on - strand at 74.195 kb on - strand at 74.299 kb on - strand at 74.314 kb on - strand at 74.353 kb on + strand at 74.375 kb on + strand at 74.433 kb on - strand at 74.440 kb on + strand at 74.441 kb on - strand at 74.447 kb on + strand at 74.447 kb on + strand at 74.491 kb on + strand, within GFF1243 at 74.491 kb on + strand, within GFF1243 at 74.690 kb on + strand, within GFF1243 at 74.691 kb on - strand, within GFF1243 at 74.696 kb on - strand, within GFF1243 at 74.745 kb on + strand, within GFF1243 at 74.745 kb on + strand, within GFF1243 at 74.785 kb on + strand, within GFF1243 at 74.872 kb on + strand, within GFF1243 at 74.923 kb on + strand, within GFF1243 at 74.924 kb on - strand, within GFF1243 at 74.926 kb on + strand, within GFF1243 at 74.926 kb on + strand, within GFF1243 at 75.061 kb on - strand, within GFF1243 at 75.061 kb on - strand, within GFF1243 at 75.184 kb on + strand, within GFF1243 at 75.262 kb on + strand at 75.262 kb on + strand at 75.279 kb on + strand at 75.282 kb on + strand at 75.298 kb on + strand at 75.371 kb on - strand at 75.391 kb on - strand, within GFF1244 at 75.422 kb on + strand, within GFF1244 at 75.432 kb on - strand, within GFF1244 at 75.659 kb on + strand, within GFF1244 at 75.739 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.772 kb on + strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.773 kb on - strand, within GFF1244 at 75.916 kb on + strand at 75.916 kb on + strand at 75.916 kb on + strand at 75.916 kb on + strand at 75.917 kb on - strand at 75.917 kb on - strand at 75.936 kb on - strand at 75.936 kb on - strand at 75.936 kb on - strand at 76.018 kb on + strand, within GFF1245 at 76.031 kb on - strand, within GFF1245 at 76.182 kb on - strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.228 kb on - strand, within GFF1245 at 76.262 kb on - strand, within GFF1245 at 76.262 kb on - strand, within GFF1245
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 73,407 - GFF1242 0.25 +1.6 73,432 + GFF1242 0.28 +0.1 73,548 + GFF1242 0.39 -0.5 73,565 + GFF1242 0.40 -0.8 73,614 + GFF1242 0.45 +0.1 73,894 - GFF1242 0.72 -0.5 73,929 - GFF1242 0.75 +0.6 73,932 + GFF1242 0.76 -0.7 73,933 - GFF1242 0.76 -0.9 73,933 - GFF1242 0.76 -0.5 73,933 - GFF1242 0.76 -0.6 73,944 - GFF1242 0.77 -0.0 73,944 - GFF1242 0.77 -0.4 73,944 - GFF1242 0.77 -0.6 74,011 - GFF1242 0.83 -1.1 74,038 + GFF1242 0.86 -0.0 74,123 + +0.5 74,195 - +0.3 74,195 - +1.3 74,195 - -0.7 74,299 - -0.7 74,314 - -0.2 74,353 + -0.7 74,375 + -1.5 74,433 - -0.4 74,440 + -0.2 74,441 - -0.7 74,447 + -0.3 74,447 + -0.1 74,491 + GFF1243 0.12 -0.0 74,491 + GFF1243 0.12 -0.5 74,690 + GFF1243 0.33 -0.0 74,691 - GFF1243 0.33 -1.5 74,696 - GFF1243 0.33 -0.3 74,745 + GFF1243 0.38 +0.1 74,745 + GFF1243 0.38 -0.1 74,785 + GFF1243 0.43 +0.2 74,872 + GFF1243 0.52 -1.1 74,923 + GFF1243 0.57 -0.7 74,924 - GFF1243 0.57 -0.3 74,926 + GFF1243 0.57 -0.5 74,926 + GFF1243 0.57 -0.8 75,061 - GFF1243 0.71 -0.2 75,061 - GFF1243 0.71 -1.1 75,184 + GFF1243 0.84 -0.1 75,262 + +0.3 75,262 + -0.8 75,279 + -0.0 75,282 + -0.6 75,298 + -0.5 75,371 - -0.3 75,391 - GFF1244 0.11 -0.3 75,422 + GFF1244 0.17 -0.1 75,432 - GFF1244 0.18 -1.2 75,659 + GFF1244 0.60 +0.7 75,739 + GFF1244 0.74 -0.6 75,772 + GFF1244 0.80 -0.1 75,772 + GFF1244 0.80 -0.1 75,772 + GFF1244 0.80 -0.9 75,772 + GFF1244 0.80 -0.1 75,772 + GFF1244 0.80 -0.3 75,773 - GFF1244 0.80 -1.7 75,773 - GFF1244 0.80 -0.3 75,773 - GFF1244 0.80 +0.1 75,773 - GFF1244 0.80 -0.0 75,773 - GFF1244 0.80 -2.1 75,773 - GFF1244 0.80 -0.7 75,773 - GFF1244 0.80 -0.4 75,773 - GFF1244 0.80 +0.4 75,916 + -0.7 75,916 + -0.3 75,916 + -1.3 75,916 + +0.0 75,917 - -0.5 75,917 - -1.7 75,936 - -0.3 75,936 - -0.1 75,936 - -0.6 76,018 + GFF1245 0.16 -0.2 76,031 - GFF1245 0.18 -0.9 76,182 - GFF1245 0.41 -1.1 76,227 + GFF1245 0.48 -2.2 76,227 + GFF1245 0.48 -0.1 76,227 + GFF1245 0.48 -0.2 76,228 - GFF1245 0.49 -0.1 76,262 - GFF1245 0.54 +0.0 76,262 - GFF1245 0.54 -0.4
Or see this region's nucleotide sequence