Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1099 and GFF1100 are separated by 100 nucleotides GFF1100 and GFF1101 are separated by 4 nucleotides GFF1101 and GFF1102 are separated by 0 nucleotides
GFF1099 - type 1 fimbriae major subunit FimA, at 285,253 to 285,795
GFF1099
GFF1100 - chaperone FimC, at 285,896 to 286,582
GFF1100
GFF1101 - type 1 fimbriae anchoring protein FimD, at 286,587 to 289,208
GFF1101
GFF1102 - Fimbriae-like adhesin SfmH, at 289,209 to 290,216
GFF1102
Position (kb)
286
287
288
289
290 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 285.623 kb on + strand, within GFF1099 at 285.624 kb on - strand, within GFF1099 at 285.887 kb on - strand at 285.890 kb on - strand at 286.166 kb on - strand, within GFF1100 at 286.177 kb on + strand, within GFF1100 at 286.306 kb on + strand, within GFF1100 at 286.312 kb on - strand, within GFF1100 at 286.419 kb on - strand, within GFF1100 at 286.439 kb on + strand, within GFF1100 at 286.439 kb on + strand, within GFF1100 at 286.469 kb on - strand, within GFF1100 at 286.587 kb on - strand at 286.699 kb on - strand at 286.786 kb on - strand at 286.800 kb on + strand at 286.812 kb on - strand at 286.925 kb on + strand, within GFF1101 at 287.052 kb on - strand, within GFF1101 at 287.091 kb on + strand, within GFF1101 at 287.092 kb on - strand, within GFF1101 at 287.092 kb on - strand, within GFF1101 at 287.219 kb on + strand, within GFF1101 at 287.319 kb on - strand, within GFF1101 at 287.322 kb on + strand, within GFF1101 at 287.373 kb on - strand, within GFF1101 at 287.373 kb on - strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.377 kb on - strand, within GFF1101 at 287.379 kb on - strand, within GFF1101 at 287.420 kb on - strand, within GFF1101 at 287.441 kb on + strand, within GFF1101 at 287.441 kb on + strand, within GFF1101 at 287.442 kb on - strand, within GFF1101 at 287.442 kb on - strand, within GFF1101 at 287.530 kb on - strand, within GFF1101 at 287.570 kb on + strand, within GFF1101 at 287.571 kb on - strand, within GFF1101 at 287.573 kb on + strand, within GFF1101 at 287.574 kb on - strand, within GFF1101 at 287.588 kb on + strand, within GFF1101 at 287.640 kb on - strand, within GFF1101 at 287.651 kb on + strand, within GFF1101 at 287.680 kb on + strand, within GFF1101 at 287.738 kb on + strand, within GFF1101 at 287.801 kb on + strand, within GFF1101 at 287.916 kb on - strand, within GFF1101 at 288.129 kb on + strand, within GFF1101 at 288.133 kb on - strand, within GFF1101 at 288.187 kb on + strand, within GFF1101 at 288.312 kb on + strand, within GFF1101 at 288.432 kb on + strand, within GFF1101 at 288.452 kb on + strand, within GFF1101 at 288.467 kb on + strand, within GFF1101 at 288.661 kb on - strand, within GFF1101 at 288.792 kb on - strand, within GFF1101 at 289.392 kb on - strand, within GFF1102 at 289.392 kb on - strand, within GFF1102 at 289.392 kb on - strand, within GFF1102 at 289.441 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.494 kb on + strand, within GFF1102 at 289.515 kb on + strand, within GFF1102 at 289.706 kb on + strand, within GFF1102 at 289.707 kb on - strand, within GFF1102 at 289.712 kb on + strand, within GFF1102 at 289.712 kb on + strand, within GFF1102 at 289.719 kb on + strand, within GFF1102 at 289.761 kb on + strand, within GFF1102 at 289.775 kb on + strand, within GFF1102 at 290.094 kb on - strand, within GFF1102 at 290.148 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 285,623 + GFF1099 0.68 +0.2 285,624 - GFF1099 0.68 +0.4 285,887 - +0.4 285,890 - +1.2 286,166 - GFF1100 0.39 -0.5 286,177 + GFF1100 0.41 +0.9 286,306 + GFF1100 0.60 +0.1 286,312 - GFF1100 0.61 -0.1 286,419 - GFF1100 0.76 +0.9 286,439 + GFF1100 0.79 +1.0 286,439 + GFF1100 0.79 +0.4 286,469 - GFF1100 0.83 +1.5 286,587 - +2.0 286,699 - +0.5 286,786 - +0.9 286,800 + +0.3 286,812 - +0.4 286,925 + GFF1101 0.13 -0.1 287,052 - GFF1101 0.18 -0.1 287,091 + GFF1101 0.19 -0.0 287,092 - GFF1101 0.19 +1.4 287,092 - GFF1101 0.19 -0.1 287,219 + GFF1101 0.24 +0.4 287,319 - GFF1101 0.28 +0.8 287,322 + GFF1101 0.28 -1.6 287,373 - GFF1101 0.30 +0.6 287,373 - GFF1101 0.30 +0.6 287,376 + GFF1101 0.30 +1.2 287,376 + GFF1101 0.30 -0.4 287,376 + GFF1101 0.30 +0.6 287,376 + GFF1101 0.30 -0.7 287,377 - GFF1101 0.30 -0.1 287,379 - GFF1101 0.30 +0.6 287,420 - GFF1101 0.32 +0.4 287,441 + GFF1101 0.33 -1.9 287,441 + GFF1101 0.33 -0.4 287,442 - GFF1101 0.33 +1.0 287,442 - GFF1101 0.33 +0.8 287,530 - GFF1101 0.36 +0.3 287,570 + GFF1101 0.37 +0.4 287,571 - GFF1101 0.38 -0.2 287,573 + GFF1101 0.38 +0.6 287,574 - GFF1101 0.38 +0.1 287,588 + GFF1101 0.38 +0.4 287,640 - GFF1101 0.40 -0.7 287,651 + GFF1101 0.41 -0.9 287,680 + GFF1101 0.42 -0.1 287,738 + GFF1101 0.44 +0.9 287,801 + GFF1101 0.46 +0.4 287,916 - GFF1101 0.51 -0.1 288,129 + GFF1101 0.59 +0.2 288,133 - GFF1101 0.59 -1.6 288,187 + GFF1101 0.61 +0.3 288,312 + GFF1101 0.66 +0.4 288,432 + GFF1101 0.70 +1.2 288,452 + GFF1101 0.71 +0.2 288,467 + GFF1101 0.72 +0.7 288,661 - GFF1101 0.79 +0.1 288,792 - GFF1101 0.84 +0.9 289,392 - GFF1102 0.18 +1.3 289,392 - GFF1102 0.18 +0.2 289,392 - GFF1102 0.18 -0.0 289,441 - GFF1102 0.23 +0.6 289,450 - GFF1102 0.24 +0.3 289,450 - GFF1102 0.24 -0.5 289,450 - GFF1102 0.24 +0.6 289,450 - GFF1102 0.24 +0.3 289,494 + GFF1102 0.28 +0.0 289,515 + GFF1102 0.30 +0.0 289,706 + GFF1102 0.49 -0.0 289,707 - GFF1102 0.49 -0.1 289,712 + GFF1102 0.50 +1.3 289,712 + GFF1102 0.50 -0.3 289,719 + GFF1102 0.51 +0.4 289,761 + GFF1102 0.55 -0.0 289,775 + GFF1102 0.56 +0.1 290,094 - GFF1102 0.88 -0.1 290,148 - +0.4
Or see this region's nucleotide sequence