Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1080 and GFF1081 are separated by 93 nucleotides GFF1081 and GFF1082 are separated by 78 nucleotides GFF1082 and GFF1083 are separated by 241 nucleotides
GFF1080 - Threonine synthase (EC 4.2.3.1), at 264,518 to 265,804
GFF1080
GFF1081 - UPF0246 protein YaaA, at 265,898 to 266,671
GFF1081
GFF1082 - Putative alanine/glycine transport protein, at 266,750 to 268,207
GFF1082
GFF1083 - Transaldolase (EC 2.2.1.2), at 268,449 to 269,402
GFF1083
Position (kb) 266
267
268
269 Strain fitness (log2 ratio) -1
0
1
2
3 at 266.061 kb on - strand, within GFF1081 at 266.303 kb on + strand, within GFF1081 at 266.304 kb on - strand, within GFF1081 at 266.304 kb on - strand, within GFF1081 at 266.329 kb on - strand, within GFF1081 at 266.329 kb on - strand, within GFF1081 at 266.345 kb on - strand, within GFF1081 at 266.399 kb on + strand, within GFF1081 at 266.409 kb on + strand, within GFF1081 at 266.671 kb on + strand at 266.672 kb on - strand at 266.834 kb on + strand at 266.834 kb on + strand at 266.835 kb on - strand at 266.860 kb on - strand at 266.877 kb on + strand at 266.877 kb on + strand at 266.877 kb on + strand at 266.878 kb on - strand at 266.878 kb on - strand at 266.878 kb on - strand at 266.878 kb on - strand at 266.926 kb on + strand, within GFF1082 at 266.958 kb on - strand, within GFF1082 at 267.073 kb on - strand, within GFF1082 at 267.077 kb on - strand, within GFF1082 at 267.092 kb on - strand, within GFF1082 at 267.273 kb on - strand, within GFF1082 at 267.400 kb on + strand, within GFF1082 at 267.401 kb on - strand, within GFF1082 at 267.522 kb on - strand, within GFF1082 at 267.543 kb on - strand, within GFF1082 at 267.608 kb on + strand, within GFF1082 at 267.784 kb on + strand, within GFF1082 at 267.785 kb on - strand, within GFF1082 at 267.787 kb on - strand, within GFF1082 at 267.787 kb on - strand, within GFF1082 at 267.832 kb on + strand, within GFF1082 at 267.985 kb on + strand, within GFF1082 at 268.149 kb on - strand at 268.223 kb on + strand at 268.243 kb on + strand at 268.243 kb on + strand at 268.261 kb on + strand at 268.262 kb on - strand at 268.287 kb on + strand at 268.287 kb on + strand at 268.287 kb on + strand at 268.302 kb on + strand at 268.328 kb on - strand at 268.328 kb on - strand at 268.392 kb on - strand at 268.528 kb on - strand at 268.544 kb on + strand at 268.545 kb on - strand, within GFF1083 at 268.545 kb on - strand, within GFF1083 at 268.595 kb on + strand, within GFF1083 at 268.731 kb on - strand, within GFF1083 at 268.796 kb on + strand, within GFF1083 at 268.907 kb on + strand, within GFF1083 at 268.984 kb on + strand, within GFF1083 at 268.985 kb on - strand, within GFF1083 at 268.991 kb on + strand, within GFF1083
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 266,061 - GFF1081 0.21 +1.0 266,303 + GFF1081 0.52 -0.5 266,304 - GFF1081 0.52 +0.2 266,304 - GFF1081 0.52 +1.0 266,329 - GFF1081 0.56 +0.6 266,329 - GFF1081 0.56 -0.5 266,345 - GFF1081 0.58 +0.1 266,399 + GFF1081 0.65 +1.8 266,409 + GFF1081 0.66 -0.3 266,671 + +0.8 266,672 - +0.5 266,834 + +1.5 266,834 + +1.7 266,835 - +0.5 266,860 - +1.4 266,877 + +1.0 266,877 + +1.3 266,877 + +1.5 266,878 - +1.9 266,878 - +0.8 266,878 - +1.5 266,878 - +1.6 266,926 + GFF1082 0.12 +1.1 266,958 - GFF1082 0.14 +1.7 267,073 - GFF1082 0.22 +1.7 267,077 - GFF1082 0.22 +1.3 267,092 - GFF1082 0.23 +1.4 267,273 - GFF1082 0.36 +1.8 267,400 + GFF1082 0.45 +0.9 267,401 - GFF1082 0.45 +2.0 267,522 - GFF1082 0.53 +1.5 267,543 - GFF1082 0.54 +1.6 267,608 + GFF1082 0.59 +1.1 267,784 + GFF1082 0.71 +1.9 267,785 - GFF1082 0.71 +2.0 267,787 - GFF1082 0.71 +1.2 267,787 - GFF1082 0.71 +2.0 267,832 + GFF1082 0.74 +1.3 267,985 + GFF1082 0.85 +1.8 268,149 - +2.0 268,223 + +1.1 268,243 + +1.3 268,243 + +0.9 268,261 + +1.5 268,262 - -0.4 268,287 + -0.0 268,287 + +0.1 268,287 + +0.4 268,302 + +1.0 268,328 - -0.0 268,328 - -0.1 268,392 - +0.1 268,528 - -0.4 268,544 + -0.2 268,545 - GFF1083 0.10 -1.6 268,545 - GFF1083 0.10 -0.1 268,595 + GFF1083 0.15 -0.1 268,731 - GFF1083 0.30 +3.0 268,796 + GFF1083 0.36 +0.1 268,907 + GFF1083 0.48 +0.9 268,984 + GFF1083 0.56 +0.7 268,985 - GFF1083 0.56 +0.6 268,991 + GFF1083 0.57 +0.7
Or see this region's nucleotide sequence