Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1025 and GFF1026 overlap by 40 nucleotides GFF1026 and GFF1027 are separated by 50 nucleotides GFF1027 and GFF1028 are separated by 150 nucleotides
GFF1025 - Putative regulator, at 209,469 to 210,194
GFF1025
GFF1026 - Transcriptional activator protein bglJ, at 210,155 to 210,829
GFF1026
GFF1027 - tRNA proofreading protein STM4549, at 210,880 to 211,338
GFF1027
GFF1028 - Ferric reductase (1.6.99.14), at 211,489 to 212,277
GFF1028
Position (kb) 210
211
212 Strain fitness (log2 ratio) -1
0
1
2
3 at 210.084 kb on + strand, within GFF1025 at 210.085 kb on - strand, within GFF1025 at 210.531 kb on + strand, within GFF1026 at 210.535 kb on + strand, within GFF1026 at 210.535 kb on + strand, within GFF1026 at 210.544 kb on + strand, within GFF1026 at 210.545 kb on - strand, within GFF1026 at 210.604 kb on + strand, within GFF1026 at 210.604 kb on + strand, within GFF1026 at 210.681 kb on + strand, within GFF1026 at 210.682 kb on - strand, within GFF1026 at 210.694 kb on + strand, within GFF1026 at 210.695 kb on - strand, within GFF1026 at 210.783 kb on + strand at 210.783 kb on + strand at 210.783 kb on + strand at 210.784 kb on - strand at 210.784 kb on - strand at 210.977 kb on - strand, within GFF1027 at 210.977 kb on - strand, within GFF1027 at 210.987 kb on - strand, within GFF1027 at 211.134 kb on + strand, within GFF1027 at 211.134 kb on + strand, within GFF1027 at 211.186 kb on + strand, within GFF1027 at 211.206 kb on + strand, within GFF1027 at 211.206 kb on + strand, within GFF1027 at 211.226 kb on - strand, within GFF1027 at 211.226 kb on - strand, within GFF1027 at 211.395 kb on - strand at 211.425 kb on + strand at 211.426 kb on - strand at 211.428 kb on - strand at 211.431 kb on - strand at 211.496 kb on - strand at 211.520 kb on - strand at 211.580 kb on - strand, within GFF1028 at 211.709 kb on - strand, within GFF1028 at 211.712 kb on + strand, within GFF1028 at 211.905 kb on + strand, within GFF1028 at 211.905 kb on + strand, within GFF1028 at 211.945 kb on + strand, within GFF1028 at 211.946 kb on - strand, within GFF1028 at 211.983 kb on - strand, within GFF1028 at 211.983 kb on - strand, within GFF1028 at 211.983 kb on - strand, within GFF1028 at 212.045 kb on + strand, within GFF1028 at 212.046 kb on - strand, within GFF1028 at 212.245 kb on - strand at 212.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 210,084 + GFF1025 0.85 -0.9 210,085 - GFF1025 0.85 +0.0 210,531 + GFF1026 0.56 +0.3 210,535 + GFF1026 0.56 +0.8 210,535 + GFF1026 0.56 +0.4 210,544 + GFF1026 0.58 +0.1 210,545 - GFF1026 0.58 -0.9 210,604 + GFF1026 0.67 -0.5 210,604 + GFF1026 0.67 -0.7 210,681 + GFF1026 0.78 -0.1 210,682 - GFF1026 0.78 -0.6 210,694 + GFF1026 0.80 +0.5 210,695 - GFF1026 0.80 +0.4 210,783 + +0.3 210,783 + +0.3 210,783 + +0.0 210,784 - +0.0 210,784 - +0.3 210,977 - GFF1027 0.21 -0.8 210,977 - GFF1027 0.21 +3.2 210,987 - GFF1027 0.23 +1.6 211,134 + GFF1027 0.55 -0.3 211,134 + GFF1027 0.55 +0.4 211,186 + GFF1027 0.67 +0.1 211,206 + GFF1027 0.71 +1.4 211,206 + GFF1027 0.71 -0.6 211,226 - GFF1027 0.75 +0.1 211,226 - GFF1027 0.75 -0.2 211,395 - -1.1 211,425 + +0.7 211,426 - +0.1 211,428 - +1.6 211,431 - +0.2 211,496 - -0.3 211,520 - +0.1 211,580 - GFF1028 0.12 +0.5 211,709 - GFF1028 0.28 +0.3 211,712 + GFF1028 0.28 +0.8 211,905 + GFF1028 0.53 +1.4 211,905 + GFF1028 0.53 +0.7 211,945 + GFF1028 0.58 +0.2 211,946 - GFF1028 0.58 -0.7 211,983 - GFF1028 0.63 +0.0 211,983 - GFF1028 0.63 +1.1 211,983 - GFF1028 0.63 +2.0 212,045 + GFF1028 0.70 +1.3 212,046 - GFF1028 0.71 +0.8 212,245 - +0.6 212,296 + +0.1
Or see this region's nucleotide sequence