Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0143 and Atu0144 are separated by 65 nucleotides Atu0144 and Atu0145 are separated by 274 nucleotides Atu0145 and Atu0146 overlap by 4 nucleotides
Atu0143: Atu0143 - MFS permease, at 147,053 to 148,375
Atu0143
Atu0144: Atu0144 - hypothetical protein, at 148,441 to 148,662
Atu0144
Atu0145: Atu0145 - glutamate synthase large subunit, at 148,937 to 154,285
Atu0145
Atu0146: Atu0146 - sulfite reductase (NADPH) flavoprotein alpha-component, at 154,282 to 156,051
Atu0146
Position (kb)
148
149
150
151
152
153
154
155 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 147.999 kb on + strand, within Atu0143 at 148.042 kb on - strand, within Atu0143 at 148.072 kb on + strand, within Atu0143 at 148.092 kb on + strand, within Atu0143 at 148.164 kb on + strand, within Atu0143 at 148.228 kb on + strand, within Atu0143 at 148.308 kb on + strand at 148.330 kb on + strand at 148.783 kb on + strand at 148.878 kb on + strand at 148.888 kb on + strand at 148.917 kb on - strand at 148.922 kb on - strand at 148.922 kb on - strand at 149.184 kb on + strand at 149.272 kb on + strand at 149.277 kb on + strand at 149.287 kb on - strand at 149.302 kb on + strand at 149.310 kb on - strand at 149.326 kb on - strand at 149.362 kb on + strand at 149.366 kb on + strand at 149.366 kb on + strand at 149.375 kb on + strand at 149.383 kb on - strand at 149.385 kb on + strand at 149.493 kb on - strand, within Atu0145 at 149.495 kb on + strand, within Atu0145 at 149.530 kb on + strand, within Atu0145 at 149.538 kb on - strand, within Atu0145 at 149.575 kb on - strand, within Atu0145 at 149.643 kb on - strand, within Atu0145 at 149.697 kb on - strand, within Atu0145 at 149.860 kb on + strand, within Atu0145 at 149.868 kb on - strand, within Atu0145 at 149.976 kb on + strand, within Atu0145 at 150.157 kb on + strand, within Atu0145 at 150.165 kb on - strand, within Atu0145 at 150.165 kb on - strand, within Atu0145 at 150.165 kb on - strand, within Atu0145 at 150.217 kb on + strand, within Atu0145 at 150.248 kb on + strand, within Atu0145 at 150.287 kb on + strand, within Atu0145 at 150.325 kb on + strand, within Atu0145 at 150.333 kb on - strand, within Atu0145 at 150.390 kb on + strand, within Atu0145 at 150.398 kb on - strand, within Atu0145 at 150.441 kb on + strand, within Atu0145 at 150.445 kb on + strand, within Atu0145 at 150.569 kb on + strand, within Atu0145 at 150.577 kb on - strand, within Atu0145 at 150.670 kb on + strand, within Atu0145 at 150.760 kb on + strand, within Atu0145 at 150.910 kb on + strand, within Atu0145 at 150.930 kb on - strand, within Atu0145 at 151.072 kb on + strand, within Atu0145 at 151.072 kb on + strand, within Atu0145 at 151.082 kb on + strand, within Atu0145 at 151.098 kb on - strand, within Atu0145 at 151.179 kb on - strand, within Atu0145 at 151.338 kb on + strand, within Atu0145 at 151.352 kb on - strand, within Atu0145 at 151.436 kb on - strand, within Atu0145 at 151.469 kb on + strand, within Atu0145 at 151.531 kb on - strand, within Atu0145 at 151.536 kb on + strand, within Atu0145 at 151.536 kb on + strand, within Atu0145 at 151.536 kb on + strand, within Atu0145 at 151.611 kb on + strand, within Atu0145 at 151.619 kb on - strand, within Atu0145 at 151.619 kb on - strand, within Atu0145 at 151.619 kb on - strand, within Atu0145 at 151.703 kb on + strand, within Atu0145 at 151.714 kb on + strand, within Atu0145 at 151.714 kb on + strand, within Atu0145 at 151.720 kb on + strand, within Atu0145 at 151.729 kb on - strand, within Atu0145 at 151.765 kb on - strand, within Atu0145 at 151.959 kb on + strand, within Atu0145 at 152.059 kb on + strand, within Atu0145 at 152.191 kb on - strand, within Atu0145 at 152.196 kb on - strand, within Atu0145 at 152.206 kb on + strand, within Atu0145 at 152.214 kb on - strand, within Atu0145 at 152.297 kb on + strand, within Atu0145 at 152.297 kb on + strand, within Atu0145 at 152.300 kb on + strand, within Atu0145 at 152.300 kb on + strand, within Atu0145 at 152.420 kb on + strand, within Atu0145 at 152.448 kb on + strand, within Atu0145 at 152.448 kb on + strand, within Atu0145 at 152.628 kb on - strand, within Atu0145 at 152.655 kb on + strand, within Atu0145 at 152.682 kb on + strand, within Atu0145 at 152.682 kb on + strand, within Atu0145 at 152.682 kb on + strand, within Atu0145 at 152.682 kb on + strand, within Atu0145 at 152.689 kb on - strand, within Atu0145 at 152.689 kb on - strand, within Atu0145 at 152.713 kb on + strand, within Atu0145 at 152.727 kb on + strand, within Atu0145 at 152.848 kb on - strand, within Atu0145 at 152.853 kb on - strand, within Atu0145 at 152.879 kb on - strand, within Atu0145 at 152.884 kb on - strand, within Atu0145 at 152.889 kb on - strand, within Atu0145 at 153.007 kb on + strand, within Atu0145 at 153.241 kb on - strand, within Atu0145 at 153.258 kb on + strand, within Atu0145 at 153.276 kb on - strand, within Atu0145 at 153.302 kb on + strand, within Atu0145 at 153.318 kb on + strand, within Atu0145 at 153.401 kb on + strand, within Atu0145 at 153.428 kb on + strand, within Atu0145 at 153.512 kb on + strand, within Atu0145 at 153.559 kb on + strand, within Atu0145 at 153.559 kb on + strand, within Atu0145 at 153.624 kb on - strand, within Atu0145 at 153.754 kb on - strand at 153.776 kb on - strand at 153.805 kb on + strand at 153.830 kb on + strand at 153.888 kb on - strand at 153.888 kb on - strand at 153.890 kb on - strand at 153.898 kb on - strand at 153.900 kb on - strand at 153.907 kb on + strand at 153.910 kb on - strand at 153.910 kb on - strand at 153.944 kb on - strand at 153.944 kb on - strand at 153.953 kb on - strand at 154.012 kb on - strand at 154.026 kb on - strand at 154.107 kb on - strand at 154.109 kb on + strand at 154.109 kb on + strand at 154.152 kb on + strand at 154.174 kb on - strand at 154.223 kb on - strand at 154.241 kb on + strand at 154.245 kb on - strand at 154.273 kb on - strand at 154.275 kb on + strand at 154.278 kb on - strand at 154.325 kb on - strand at 154.557 kb on - strand, within Atu0146 at 154.654 kb on + strand, within Atu0146 at 154.757 kb on + strand, within Atu0146 at 154.840 kb on + strand, within Atu0146 at 154.848 kb on - strand, within Atu0146 at 154.953 kb on + strand, within Atu0146 at 154.953 kb on + strand, within Atu0146 at 154.994 kb on + strand, within Atu0146 at 155.012 kb on - strand, within Atu0146 at 155.037 kb on + strand, within Atu0146 at 155.078 kb on + strand, within Atu0146 at 155.124 kb on - strand, within Atu0146 at 155.275 kb on - strand, within Atu0146 at 155.276 kb on - strand, within Atu0146
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 147,999 + Atu0143 0.72 +0.5 148,042 - Atu0143 0.75 +0.5 148,072 + Atu0143 0.77 +0.1 148,092 + Atu0143 0.79 -0.1 148,164 + Atu0143 0.84 +0.0 148,228 + Atu0143 0.89 +0.0 148,308 + -0.5 148,330 + -0.4 148,783 + +0.6 148,878 + -0.1 148,888 + +1.3 148,917 - -0.2 148,922 - +1.0 148,922 - -0.3 149,184 + +2.3 149,272 + +0.4 149,277 + -0.1 149,287 - -0.6 149,302 + +0.0 149,310 - -2.1 149,326 - +0.6 149,362 + -0.6 149,366 + -0.0 149,366 + -0.3 149,375 + +1.8 149,383 - +0.5 149,385 + -0.1 149,493 - Atu0145 0.10 +0.1 149,495 + Atu0145 0.10 -0.1 149,530 + Atu0145 0.11 -0.0 149,538 - Atu0145 0.11 -0.0 149,575 - Atu0145 0.12 -0.4 149,643 - Atu0145 0.13 -0.3 149,697 - Atu0145 0.14 -0.1 149,860 + Atu0145 0.17 +0.3 149,868 - Atu0145 0.17 -1.4 149,976 + Atu0145 0.19 -0.0 150,157 + Atu0145 0.23 -0.1 150,165 - Atu0145 0.23 +0.6 150,165 - Atu0145 0.23 +0.3 150,165 - Atu0145 0.23 +0.2 150,217 + Atu0145 0.24 +0.8 150,248 + Atu0145 0.25 -0.8 150,287 + Atu0145 0.25 +0.2 150,325 + Atu0145 0.26 +0.1 150,333 - Atu0145 0.26 +0.5 150,390 + Atu0145 0.27 +0.3 150,398 - Atu0145 0.27 +0.9 150,441 + Atu0145 0.28 +0.1 150,445 + Atu0145 0.28 +0.3 150,569 + Atu0145 0.31 +0.1 150,577 - Atu0145 0.31 -0.1 150,670 + Atu0145 0.32 +0.2 150,760 + Atu0145 0.34 -0.2 150,910 + Atu0145 0.37 +0.1 150,930 - Atu0145 0.37 -0.0 151,072 + Atu0145 0.40 +0.2 151,072 + Atu0145 0.40 +0.9 151,082 + Atu0145 0.40 -0.3 151,098 - Atu0145 0.40 +0.1 151,179 - Atu0145 0.42 -0.2 151,338 + Atu0145 0.45 -0.3 151,352 - Atu0145 0.45 +0.2 151,436 - Atu0145 0.47 +0.3 151,469 + Atu0145 0.47 -0.2 151,531 - Atu0145 0.48 +0.7 151,536 + Atu0145 0.49 +0.1 151,536 + Atu0145 0.49 -0.1 151,536 + Atu0145 0.49 -0.2 151,611 + Atu0145 0.50 +0.3 151,619 - Atu0145 0.50 -0.3 151,619 - Atu0145 0.50 -0.2 151,619 - Atu0145 0.50 -0.4 151,703 + Atu0145 0.52 +0.9 151,714 + Atu0145 0.52 -0.1 151,714 + Atu0145 0.52 -0.1 151,720 + Atu0145 0.52 -0.7 151,729 - Atu0145 0.52 +0.0 151,765 - Atu0145 0.53 -0.7 151,959 + Atu0145 0.56 -0.1 152,059 + Atu0145 0.58 +0.8 152,191 - Atu0145 0.61 +0.8 152,196 - Atu0145 0.61 -0.0 152,206 + Atu0145 0.61 -0.1 152,214 - Atu0145 0.61 +0.5 152,297 + Atu0145 0.63 -0.0 152,297 + Atu0145 0.63 -0.3 152,300 + Atu0145 0.63 -0.6 152,300 + Atu0145 0.63 +0.3 152,420 + Atu0145 0.65 +0.3 152,448 + Atu0145 0.66 -0.1 152,448 + Atu0145 0.66 -1.0 152,628 - Atu0145 0.69 -1.1 152,655 + Atu0145 0.70 +1.8 152,682 + Atu0145 0.70 +0.6 152,682 + Atu0145 0.70 +0.3 152,682 + Atu0145 0.70 +0.0 152,682 + Atu0145 0.70 +0.4 152,689 - Atu0145 0.70 +0.1 152,689 - Atu0145 0.70 -0.0 152,713 + Atu0145 0.71 +0.1 152,727 + Atu0145 0.71 -0.1 152,848 - Atu0145 0.73 -0.0 152,853 - Atu0145 0.73 +0.3 152,879 - Atu0145 0.74 +0.3 152,884 - Atu0145 0.74 +0.2 152,889 - Atu0145 0.74 +0.1 153,007 + Atu0145 0.76 -0.4 153,241 - Atu0145 0.80 +0.2 153,258 + Atu0145 0.81 -0.0 153,276 - Atu0145 0.81 -0.3 153,302 + Atu0145 0.82 -0.2 153,318 + Atu0145 0.82 -0.7 153,401 + Atu0145 0.83 -1.3 153,428 + Atu0145 0.84 -1.3 153,512 + Atu0145 0.86 +0.5 153,559 + Atu0145 0.86 +0.0 153,559 + Atu0145 0.86 -0.1 153,624 - Atu0145 0.88 -1.3 153,754 - -0.4 153,776 - -0.4 153,805 + -0.0 153,830 + -0.4 153,888 - -0.3 153,888 - +0.2 153,890 - -0.4 153,898 - +0.5 153,900 - +0.1 153,907 + +0.4 153,910 - -0.6 153,910 - +0.6 153,944 - -0.1 153,944 - -0.4 153,953 - -1.1 154,012 - +0.4 154,026 - -1.2 154,107 - -0.5 154,109 + -0.7 154,109 + +0.3 154,152 + -0.0 154,174 - -0.4 154,223 - -0.4 154,241 + -0.7 154,245 - +0.1 154,273 - -0.2 154,275 + +0.7 154,278 - +0.2 154,325 - +0.1 154,557 - Atu0146 0.16 +0.1 154,654 + Atu0146 0.21 +0.0 154,757 + Atu0146 0.27 -0.6 154,840 + Atu0146 0.32 -0.9 154,848 - Atu0146 0.32 -0.1 154,953 + Atu0146 0.38 +0.4 154,953 + Atu0146 0.38 +0.2 154,994 + Atu0146 0.40 -0.1 155,012 - Atu0146 0.41 +0.1 155,037 + Atu0146 0.43 -0.1 155,078 + Atu0146 0.45 -0.2 155,124 - Atu0146 0.48 +0.4 155,275 - Atu0146 0.56 +0.4 155,276 - Atu0146 0.56 +0.3
Or see this region's nucleotide sequence