Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2257 and PfGW456L13_2258 overlap by 4 nucleotides PfGW456L13_2258 and PfGW456L13_2259 are separated by 0 nucleotides
PfGW456L13_2257 - putative ATP/GTP-binding protein, at 2,470,663 to 2,471,553
_2257
PfGW456L13_2258 - Free methionine-(R)-sulfoxide reductase, contains GAF domain, at 2,471,550 to 2,472,032
_2258
PfGW456L13_2259 - Adenylate cyclase (EC 4.6.1.1), at 2,472,033 to 2,474,978
_2259
Position (kb)
2471
2472
2473 Strain fitness (log2 ratio)
-2
-1
0
1 at 2470.581 kb on - strand at 2470.624 kb on + strand at 2470.625 kb on - strand at 2470.625 kb on - strand at 2470.625 kb on - strand at 2470.636 kb on + strand at 2470.636 kb on + strand at 2470.637 kb on - strand at 2470.637 kb on - strand at 2471.197 kb on + strand, within PfGW456L13_2257 at 2471.197 kb on + strand, within PfGW456L13_2257 at 2471.197 kb on + strand, within PfGW456L13_2257 at 2471.197 kb on + strand, within PfGW456L13_2257 at 2471.197 kb on + strand, within PfGW456L13_2257 at 2471.198 kb on - strand, within PfGW456L13_2257 at 2471.515 kb on + strand at 2471.560 kb on + strand at 2471.561 kb on - strand at 2471.599 kb on + strand, within PfGW456L13_2258 at 2471.599 kb on + strand, within PfGW456L13_2258 at 2471.599 kb on + strand, within PfGW456L13_2258 at 2471.599 kb on + strand, within PfGW456L13_2258 at 2471.600 kb on - strand, within PfGW456L13_2258 at 2471.600 kb on - strand, within PfGW456L13_2258 at 2471.600 kb on - strand, within PfGW456L13_2258 at 2471.600 kb on - strand, within PfGW456L13_2258 at 2471.600 kb on - strand, within PfGW456L13_2258 at 2471.600 kb on - strand, within PfGW456L13_2258 at 2471.713 kb on - strand, within PfGW456L13_2258 at 2472.115 kb on + strand at 2472.124 kb on + strand at 2472.124 kb on + strand at 2472.124 kb on + strand at 2472.124 kb on + strand at 2472.125 kb on - strand at 2472.125 kb on - strand at 2472.125 kb on - strand at 2472.125 kb on - strand at 2472.266 kb on + strand at 2472.367 kb on - strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.451 kb on + strand, within PfGW456L13_2259 at 2472.452 kb on - strand, within PfGW456L13_2259 at 2472.452 kb on - strand, within PfGW456L13_2259 at 2472.452 kb on - strand, within PfGW456L13_2259 at 2472.452 kb on - strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.496 kb on + strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.497 kb on - strand, within PfGW456L13_2259 at 2472.514 kb on + strand, within PfGW456L13_2259 at 2472.514 kb on + strand, within PfGW456L13_2259 at 2472.514 kb on + strand, within PfGW456L13_2259 at 2472.514 kb on + strand, within PfGW456L13_2259 at 2472.515 kb on - strand, within PfGW456L13_2259 at 2472.515 kb on - strand, within PfGW456L13_2259 at 2472.515 kb on - strand, within PfGW456L13_2259 at 2472.515 kb on - strand, within PfGW456L13_2259 at 2472.883 kb on + strand, within PfGW456L13_2259 at 2472.883 kb on + strand, within PfGW456L13_2259 at 2472.909 kb on + strand, within PfGW456L13_2259 at 2472.909 kb on + strand, within PfGW456L13_2259 at 2472.909 kb on + strand, within PfGW456L13_2259 at 2472.909 kb on + strand, within PfGW456L13_2259 at 2472.982 kb on + strand, within PfGW456L13_2259 at 2472.982 kb on + strand, within PfGW456L13_2259 at 2472.982 kb on + strand, within PfGW456L13_2259 at 2472.982 kb on + strand, within PfGW456L13_2259 at 2472.983 kb on - strand, within PfGW456L13_2259 at 2472.983 kb on - strand, within PfGW456L13_2259 at 2472.983 kb on - strand, within PfGW456L13_2259 at 2472.983 kb on - strand, within PfGW456L13_2259 at 2472.983 kb on - strand, within PfGW456L13_2259 at 2472.983 kb on - strand, within PfGW456L13_2259
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 2,470,581 - +0.3 2,470,624 + -0.8 2,470,625 - -0.9 2,470,625 - +0.0 2,470,625 - -1.4 2,470,636 + +0.5 2,470,636 + -0.4 2,470,637 - -1.8 2,470,637 - -1.2 2,471,197 + PfGW456L13_2257 0.60 +0.1 2,471,197 + PfGW456L13_2257 0.60 -2.0 2,471,197 + PfGW456L13_2257 0.60 +0.3 2,471,197 + PfGW456L13_2257 0.60 +0.0 2,471,197 + PfGW456L13_2257 0.60 -0.6 2,471,198 - PfGW456L13_2257 0.60 -0.7 2,471,515 + +0.6 2,471,560 + +0.5 2,471,561 - +0.6 2,471,599 + PfGW456L13_2258 0.10 -1.1 2,471,599 + PfGW456L13_2258 0.10 +1.0 2,471,599 + PfGW456L13_2258 0.10 -0.6 2,471,599 + PfGW456L13_2258 0.10 -0.4 2,471,600 - PfGW456L13_2258 0.10 -1.4 2,471,600 - PfGW456L13_2258 0.10 -1.0 2,471,600 - PfGW456L13_2258 0.10 -0.5 2,471,600 - PfGW456L13_2258 0.10 -0.7 2,471,600 - PfGW456L13_2258 0.10 -1.0 2,471,600 - PfGW456L13_2258 0.10 -1.3 2,471,713 - PfGW456L13_2258 0.34 -1.0 2,472,115 + -0.9 2,472,124 + -0.0 2,472,124 + +0.3 2,472,124 + -0.2 2,472,124 + +0.4 2,472,125 - -1.3 2,472,125 - +0.1 2,472,125 - +1.2 2,472,125 - -1.0 2,472,266 + +0.3 2,472,367 - PfGW456L13_2259 0.11 -0.9 2,472,451 + PfGW456L13_2259 0.14 -0.1 2,472,451 + PfGW456L13_2259 0.14 -1.2 2,472,451 + PfGW456L13_2259 0.14 -0.4 2,472,451 + PfGW456L13_2259 0.14 +0.0 2,472,451 + PfGW456L13_2259 0.14 -0.5 2,472,451 + PfGW456L13_2259 0.14 -0.0 2,472,451 + PfGW456L13_2259 0.14 +0.8 2,472,452 - PfGW456L13_2259 0.14 +1.1 2,472,452 - PfGW456L13_2259 0.14 +1.3 2,472,452 - PfGW456L13_2259 0.14 +0.3 2,472,452 - PfGW456L13_2259 0.14 -1.0 2,472,496 + PfGW456L13_2259 0.16 -0.0 2,472,496 + PfGW456L13_2259 0.16 -0.1 2,472,496 + PfGW456L13_2259 0.16 -1.1 2,472,496 + PfGW456L13_2259 0.16 +0.5 2,472,496 + PfGW456L13_2259 0.16 -0.2 2,472,496 + PfGW456L13_2259 0.16 -0.4 2,472,496 + PfGW456L13_2259 0.16 -0.5 2,472,496 + PfGW456L13_2259 0.16 -1.1 2,472,497 - PfGW456L13_2259 0.16 +1.6 2,472,497 - PfGW456L13_2259 0.16 -1.3 2,472,497 - PfGW456L13_2259 0.16 +0.9 2,472,497 - PfGW456L13_2259 0.16 -0.3 2,472,497 - PfGW456L13_2259 0.16 -0.8 2,472,497 - PfGW456L13_2259 0.16 -0.9 2,472,497 - PfGW456L13_2259 0.16 -1.6 2,472,514 + PfGW456L13_2259 0.16 -0.2 2,472,514 + PfGW456L13_2259 0.16 +1.3 2,472,514 + PfGW456L13_2259 0.16 +0.9 2,472,514 + PfGW456L13_2259 0.16 +1.3 2,472,515 - PfGW456L13_2259 0.16 -0.2 2,472,515 - PfGW456L13_2259 0.16 -1.3 2,472,515 - PfGW456L13_2259 0.16 -0.2 2,472,515 - PfGW456L13_2259 0.16 +0.4 2,472,883 + PfGW456L13_2259 0.29 -0.4 2,472,883 + PfGW456L13_2259 0.29 -2.3 2,472,909 + PfGW456L13_2259 0.30 -1.2 2,472,909 + PfGW456L13_2259 0.30 +0.2 2,472,909 + PfGW456L13_2259 0.30 -0.3 2,472,909 + PfGW456L13_2259 0.30 +0.2 2,472,982 + PfGW456L13_2259 0.32 -1.0 2,472,982 + PfGW456L13_2259 0.32 +1.1 2,472,982 + PfGW456L13_2259 0.32 -0.2 2,472,982 + PfGW456L13_2259 0.32 +0.1 2,472,983 - PfGW456L13_2259 0.32 +0.7 2,472,983 - PfGW456L13_2259 0.32 -0.2 2,472,983 - PfGW456L13_2259 0.32 -0.7 2,472,983 - PfGW456L13_2259 0.32 -1.5 2,472,983 - PfGW456L13_2259 0.32 -0.3 2,472,983 - PfGW456L13_2259 0.32 -0.1
Or see this region's nucleotide sequence