Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4157 and Atu4159 are separated by 175 nucleotides Atu4159 and Atu4160 are separated by 67 nucleotides Atu4160 and Atu4161 overlap by 1 nucleotides Atu4161 and Atu4162 are separated by 137 nucleotides
Atu4157: Atu4157 - bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 1,274,540 to 1,278,226
Atu4157
Atu4159: Atu4159 - AsnC family transcriptional regulator, at 1,278,402 to 1,278,872
Atu4159
Atu4160: Atu4160 - RNA polymerase sigma factor, at 1,278,940 to 1,279,506
Atu4160
Atu4161: Atu4161 - hypothetical protein, at 1,279,506 to 1,279,712
Atu4161
Atu4162: Atu4162 - two-component response regulator, at 1,279,850 to 1,280,644
Atu4162
Position (kb)
1278
1279
1280 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1278.032 kb on + strand at 1278.041 kb on - strand at 1278.041 kb on - strand at 1278.184 kb on - strand at 1278.248 kb on + strand at 1278.256 kb on - strand at 1278.271 kb on + strand at 1278.560 kb on + strand, within Atu4159 at 1278.653 kb on + strand, within Atu4159 at 1278.684 kb on - strand, within Atu4159 at 1278.694 kb on - strand, within Atu4159 at 1278.708 kb on - strand, within Atu4159 at 1278.742 kb on + strand, within Atu4159 at 1278.825 kb on + strand, within Atu4159 at 1278.826 kb on - strand at 1278.840 kb on + strand at 1278.968 kb on + strand at 1278.976 kb on - strand at 1278.976 kb on - strand at 1279.035 kb on + strand, within Atu4160 at 1279.054 kb on + strand, within Atu4160 at 1279.062 kb on - strand, within Atu4160 at 1279.232 kb on + strand, within Atu4160 at 1279.232 kb on + strand, within Atu4160 at 1279.240 kb on - strand, within Atu4160 at 1279.240 kb on - strand, within Atu4160 at 1279.240 kb on - strand, within Atu4160 at 1279.383 kb on + strand, within Atu4160 at 1279.392 kb on + strand, within Atu4160 at 1279.400 kb on - strand, within Atu4160 at 1279.404 kb on + strand, within Atu4160 at 1279.521 kb on - strand at 1279.544 kb on - strand, within Atu4161 at 1279.614 kb on - strand, within Atu4161 at 1279.882 kb on + strand at 1279.939 kb on - strand, within Atu4162 at 1280.056 kb on - strand, within Atu4162 at 1280.059 kb on + strand, within Atu4162 at 1280.110 kb on + strand, within Atu4162 at 1280.110 kb on + strand, within Atu4162 at 1280.110 kb on + strand, within Atu4162 at 1280.118 kb on - strand, within Atu4162 at 1280.157 kb on + strand, within Atu4162 at 1280.159 kb on + strand, within Atu4162 at 1280.165 kb on - strand, within Atu4162 at 1280.199 kb on - strand, within Atu4162 at 1280.215 kb on + strand, within Atu4162 at 1280.223 kb on - strand, within Atu4162 at 1280.301 kb on - strand, within Atu4162 at 1280.302 kb on - strand, within Atu4162 at 1280.328 kb on - strand, within Atu4162 at 1280.386 kb on + strand, within Atu4162
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 1,278,032 + -3.3 1,278,041 - -4.5 1,278,041 - -2.0 1,278,184 - -3.7 1,278,248 + -0.7 1,278,256 - -2.3 1,278,271 + -5.1 1,278,560 + Atu4159 0.34 +0.6 1,278,653 + Atu4159 0.53 -3.2 1,278,684 - Atu4159 0.60 -2.0 1,278,694 - Atu4159 0.62 -2.7 1,278,708 - Atu4159 0.65 -2.4 1,278,742 + Atu4159 0.72 -1.7 1,278,825 + Atu4159 0.90 -3.0 1,278,826 - -0.7 1,278,840 + -3.2 1,278,968 + -1.3 1,278,976 - -0.0 1,278,976 - -0.4 1,279,035 + Atu4160 0.17 -0.1 1,279,054 + Atu4160 0.20 -0.1 1,279,062 - Atu4160 0.22 +2.1 1,279,232 + Atu4160 0.51 +0.6 1,279,232 + Atu4160 0.51 -0.1 1,279,240 - Atu4160 0.53 +0.2 1,279,240 - Atu4160 0.53 -1.0 1,279,240 - Atu4160 0.53 -0.2 1,279,383 + Atu4160 0.78 +0.1 1,279,392 + Atu4160 0.80 -0.3 1,279,400 - Atu4160 0.81 -0.1 1,279,404 + Atu4160 0.82 -0.4 1,279,521 - +0.2 1,279,544 - Atu4161 0.18 +1.2 1,279,614 - Atu4161 0.52 -0.3 1,279,882 + -0.4 1,279,939 - Atu4162 0.11 -1.1 1,280,056 - Atu4162 0.26 -0.4 1,280,059 + Atu4162 0.26 +0.7 1,280,110 + Atu4162 0.33 -0.2 1,280,110 + Atu4162 0.33 -0.9 1,280,110 + Atu4162 0.33 +0.6 1,280,118 - Atu4162 0.34 +0.2 1,280,157 + Atu4162 0.39 -0.3 1,280,159 + Atu4162 0.39 -0.5 1,280,165 - Atu4162 0.40 +1.1 1,280,199 - Atu4162 0.44 +1.6 1,280,215 + Atu4162 0.46 -0.4 1,280,223 - Atu4162 0.47 -0.0 1,280,301 - Atu4162 0.57 -0.3 1,280,302 - Atu4162 0.57 +0.4 1,280,328 - Atu4162 0.60 +0.2 1,280,386 + Atu4162 0.67 -0.9
Or see this region's nucleotide sequence