Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2311 and B158DRAFT_2312 are separated by 114 nucleotides B158DRAFT_2312 and B158DRAFT_2313 are separated by 15 nucleotides
B158DRAFT_2311: B158DRAFT_2311 - ribosomal large subunit pseudouridine synthase B (EC 5.4.99.-), at 471,340 to 472,143
_2311
B158DRAFT_2312: B158DRAFT_2312 - polyphosphate kinase 1, at 472,258 to 474,393
_2312
B158DRAFT_2313: B158DRAFT_2313 - Exopolyphosphatase, at 474,409 to 475,956
_2313
Position (kb)
472
473
474
475 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 471.294 kb on - strand at 471.344 kb on - strand at 471.384 kb on - strand at 471.384 kb on - strand at 471.384 kb on - strand at 471.384 kb on - strand at 471.390 kb on - strand at 471.537 kb on + strand, within B158DRAFT_2311 at 471.549 kb on + strand, within B158DRAFT_2311 at 471.549 kb on + strand, within B158DRAFT_2311 at 471.697 kb on + strand, within B158DRAFT_2311 at 471.809 kb on - strand, within B158DRAFT_2311 at 471.860 kb on - strand, within B158DRAFT_2311 at 471.904 kb on + strand, within B158DRAFT_2311 at 471.904 kb on + strand, within B158DRAFT_2311 at 471.904 kb on + strand, within B158DRAFT_2311 at 471.904 kb on + strand, within B158DRAFT_2311 at 471.911 kb on + strand, within B158DRAFT_2311 at 471.912 kb on - strand, within B158DRAFT_2311 at 471.912 kb on - strand, within B158DRAFT_2311 at 471.912 kb on - strand, within B158DRAFT_2311 at 471.912 kb on - strand, within B158DRAFT_2311 at 471.912 kb on - strand, within B158DRAFT_2311 at 471.952 kb on - strand, within B158DRAFT_2311 at 472.027 kb on - strand, within B158DRAFT_2311 at 472.255 kb on + strand at 472.304 kb on + strand at 472.304 kb on + strand at 472.312 kb on - strand at 472.312 kb on - strand at 472.312 kb on - strand at 472.317 kb on - strand at 472.318 kb on + strand at 472.550 kb on + strand, within B158DRAFT_2312 at 472.558 kb on + strand, within B158DRAFT_2312 at 472.563 kb on - strand, within B158DRAFT_2312 at 472.601 kb on - strand, within B158DRAFT_2312 at 472.632 kb on - strand, within B158DRAFT_2312 at 472.749 kb on + strand, within B158DRAFT_2312 at 472.756 kb on + strand, within B158DRAFT_2312 at 472.764 kb on - strand, within B158DRAFT_2312 at 472.775 kb on + strand, within B158DRAFT_2312 at 472.783 kb on - strand, within B158DRAFT_2312 at 472.783 kb on - strand, within B158DRAFT_2312 at 472.879 kb on + strand, within B158DRAFT_2312 at 472.887 kb on - strand, within B158DRAFT_2312 at 473.083 kb on + strand, within B158DRAFT_2312 at 473.083 kb on + strand, within B158DRAFT_2312 at 473.091 kb on - strand, within B158DRAFT_2312 at 473.095 kb on + strand, within B158DRAFT_2312 at 473.142 kb on - strand, within B158DRAFT_2312 at 473.157 kb on + strand, within B158DRAFT_2312 at 473.287 kb on + strand, within B158DRAFT_2312 at 473.295 kb on - strand, within B158DRAFT_2312 at 473.317 kb on - strand, within B158DRAFT_2312 at 473.438 kb on + strand, within B158DRAFT_2312 at 473.469 kb on + strand, within B158DRAFT_2312 at 473.510 kb on - strand, within B158DRAFT_2312 at 473.535 kb on + strand, within B158DRAFT_2312 at 473.701 kb on + strand, within B158DRAFT_2312 at 473.708 kb on - strand, within B158DRAFT_2312 at 473.710 kb on + strand, within B158DRAFT_2312 at 473.718 kb on - strand, within B158DRAFT_2312 at 473.718 kb on - strand, within B158DRAFT_2312 at 473.748 kb on + strand, within B158DRAFT_2312 at 473.748 kb on + strand, within B158DRAFT_2312 at 473.748 kb on + strand, within B158DRAFT_2312 at 473.748 kb on + strand, within B158DRAFT_2312 at 473.756 kb on - strand, within B158DRAFT_2312 at 473.756 kb on - strand, within B158DRAFT_2312 at 473.756 kb on - strand, within B158DRAFT_2312 at 473.972 kb on - strand, within B158DRAFT_2312 at 473.992 kb on + strand, within B158DRAFT_2312 at 473.992 kb on + strand, within B158DRAFT_2312 at 473.992 kb on + strand, within B158DRAFT_2312 at 473.992 kb on + strand, within B158DRAFT_2312 at 473.992 kb on + strand, within B158DRAFT_2312 at 473.992 kb on + strand, within B158DRAFT_2312 at 474.000 kb on - strand, within B158DRAFT_2312 at 474.000 kb on - strand, within B158DRAFT_2312 at 474.000 kb on - strand, within B158DRAFT_2312 at 474.005 kb on - strand, within B158DRAFT_2312 at 474.086 kb on + strand, within B158DRAFT_2312 at 474.116 kb on + strand, within B158DRAFT_2312 at 474.204 kb on + strand at 474.232 kb on + strand at 474.237 kb on + strand at 474.294 kb on + strand at 474.386 kb on - strand at 474.390 kb on - strand at 474.409 kb on + strand at 474.454 kb on + strand at 474.671 kb on + strand, within B158DRAFT_2313 at 474.671 kb on + strand, within B158DRAFT_2313 at 474.671 kb on + strand, within B158DRAFT_2313 at 474.671 kb on + strand, within B158DRAFT_2313 at 474.679 kb on - strand, within B158DRAFT_2313 at 474.679 kb on - strand, within B158DRAFT_2313 at 474.679 kb on - strand, within B158DRAFT_2313 at 474.744 kb on - strand, within B158DRAFT_2313 at 474.749 kb on - strand, within B158DRAFT_2313 at 474.772 kb on + strand, within B158DRAFT_2313 at 474.780 kb on - strand, within B158DRAFT_2313 at 474.780 kb on - strand, within B158DRAFT_2313 at 474.780 kb on - strand, within B158DRAFT_2313 at 474.780 kb on - strand, within B158DRAFT_2313 at 474.791 kb on - strand, within B158DRAFT_2313 at 474.908 kb on + strand, within B158DRAFT_2313 at 474.916 kb on - strand, within B158DRAFT_2313 at 474.923 kb on + strand, within B158DRAFT_2313 at 474.961 kb on - strand, within B158DRAFT_2313 at 474.963 kb on + strand, within B158DRAFT_2313 at 474.963 kb on + strand, within B158DRAFT_2313 at 474.971 kb on - strand, within B158DRAFT_2313 at 474.976 kb on - strand, within B158DRAFT_2313 at 474.997 kb on - strand, within B158DRAFT_2313 at 475.025 kb on - strand, within B158DRAFT_2313 at 475.054 kb on - strand, within B158DRAFT_2313 at 475.093 kb on + strand, within B158DRAFT_2313 at 475.210 kb on + strand, within B158DRAFT_2313 at 475.210 kb on + strand, within B158DRAFT_2313 at 475.217 kb on - strand, within B158DRAFT_2313 at 475.218 kb on - strand, within B158DRAFT_2313 at 475.218 kb on - strand, within B158DRAFT_2313 at 475.218 kb on - strand, within B158DRAFT_2313 at 475.218 kb on - strand, within B158DRAFT_2313 at 475.218 kb on - strand, within B158DRAFT_2313 at 475.224 kb on - strand, within B158DRAFT_2313 at 475.236 kb on - strand, within B158DRAFT_2313 at 475.238 kb on + strand, within B158DRAFT_2313 at 475.283 kb on - strand, within B158DRAFT_2313 at 475.283 kb on - strand, within B158DRAFT_2313 at 475.297 kb on + strand, within B158DRAFT_2313 at 475.305 kb on - strand, within B158DRAFT_2313 at 475.305 kb on - strand, within B158DRAFT_2313 at 475.305 kb on - strand, within B158DRAFT_2313
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 471,294 - -0.8 471,344 - -0.2 471,384 - -1.1 471,384 - -0.0 471,384 - -0.8 471,384 - -1.2 471,390 - -0.1 471,537 + B158DRAFT_2311 0.25 -0.9 471,549 + B158DRAFT_2311 0.26 -0.5 471,549 + B158DRAFT_2311 0.26 +1.1 471,697 + B158DRAFT_2311 0.44 -0.9 471,809 - B158DRAFT_2311 0.58 -0.2 471,860 - B158DRAFT_2311 0.65 +1.2 471,904 + B158DRAFT_2311 0.70 -0.4 471,904 + B158DRAFT_2311 0.70 -1.3 471,904 + B158DRAFT_2311 0.70 -1.0 471,904 + B158DRAFT_2311 0.70 -1.5 471,911 + B158DRAFT_2311 0.71 +1.2 471,912 - B158DRAFT_2311 0.71 -0.1 471,912 - B158DRAFT_2311 0.71 +0.5 471,912 - B158DRAFT_2311 0.71 +0.9 471,912 - B158DRAFT_2311 0.71 -1.4 471,912 - B158DRAFT_2311 0.71 +0.9 471,952 - B158DRAFT_2311 0.76 -0.4 472,027 - B158DRAFT_2311 0.85 +0.6 472,255 + +1.6 472,304 + -0.7 472,304 + -1.4 472,312 - +0.6 472,312 - +1.8 472,312 - -0.1 472,317 - -0.5 472,318 + -1.3 472,550 + B158DRAFT_2312 0.14 -0.2 472,558 + B158DRAFT_2312 0.14 -1.7 472,563 - B158DRAFT_2312 0.14 +0.7 472,601 - B158DRAFT_2312 0.16 -1.0 472,632 - B158DRAFT_2312 0.18 +1.9 472,749 + B158DRAFT_2312 0.23 -0.8 472,756 + B158DRAFT_2312 0.23 -0.8 472,764 - B158DRAFT_2312 0.24 -2.3 472,775 + B158DRAFT_2312 0.24 +0.2 472,783 - B158DRAFT_2312 0.25 -0.5 472,783 - B158DRAFT_2312 0.25 -0.3 472,879 + B158DRAFT_2312 0.29 +0.3 472,887 - B158DRAFT_2312 0.29 -0.6 473,083 + B158DRAFT_2312 0.39 -0.5 473,083 + B158DRAFT_2312 0.39 +0.5 473,091 - B158DRAFT_2312 0.39 -0.2 473,095 + B158DRAFT_2312 0.39 -1.3 473,142 - B158DRAFT_2312 0.41 -1.8 473,157 + B158DRAFT_2312 0.42 -1.4 473,287 + B158DRAFT_2312 0.48 +0.1 473,295 - B158DRAFT_2312 0.49 +0.0 473,317 - B158DRAFT_2312 0.50 -0.1 473,438 + B158DRAFT_2312 0.55 +1.4 473,469 + B158DRAFT_2312 0.57 -0.3 473,510 - B158DRAFT_2312 0.59 -0.8 473,535 + B158DRAFT_2312 0.60 -1.0 473,701 + B158DRAFT_2312 0.68 -0.2 473,708 - B158DRAFT_2312 0.68 +1.6 473,710 + B158DRAFT_2312 0.68 -1.0 473,718 - B158DRAFT_2312 0.68 -0.3 473,718 - B158DRAFT_2312 0.68 -1.2 473,748 + B158DRAFT_2312 0.70 -0.6 473,748 + B158DRAFT_2312 0.70 -0.3 473,748 + B158DRAFT_2312 0.70 +0.7 473,748 + B158DRAFT_2312 0.70 -0.6 473,756 - B158DRAFT_2312 0.70 +0.2 473,756 - B158DRAFT_2312 0.70 -0.5 473,756 - B158DRAFT_2312 0.70 +0.5 473,972 - B158DRAFT_2312 0.80 +0.6 473,992 + B158DRAFT_2312 0.81 -0.7 473,992 + B158DRAFT_2312 0.81 -0.6 473,992 + B158DRAFT_2312 0.81 -0.6 473,992 + B158DRAFT_2312 0.81 +0.2 473,992 + B158DRAFT_2312 0.81 -1.0 473,992 + B158DRAFT_2312 0.81 -0.6 474,000 - B158DRAFT_2312 0.82 -1.0 474,000 - B158DRAFT_2312 0.82 -0.4 474,000 - B158DRAFT_2312 0.82 -0.3 474,005 - B158DRAFT_2312 0.82 -0.6 474,086 + B158DRAFT_2312 0.86 -1.3 474,116 + B158DRAFT_2312 0.87 -0.3 474,204 + -0.8 474,232 + -0.4 474,237 + +0.5 474,294 + +1.1 474,386 - -0.0 474,390 - -0.7 474,409 + -0.9 474,454 + -0.7 474,671 + B158DRAFT_2313 0.17 -0.7 474,671 + B158DRAFT_2313 0.17 -0.1 474,671 + B158DRAFT_2313 0.17 -0.4 474,671 + B158DRAFT_2313 0.17 -0.6 474,679 - B158DRAFT_2313 0.17 -1.8 474,679 - B158DRAFT_2313 0.17 -0.0 474,679 - B158DRAFT_2313 0.17 -1.4 474,744 - B158DRAFT_2313 0.22 -1.0 474,749 - B158DRAFT_2313 0.22 -2.5 474,772 + B158DRAFT_2313 0.23 -1.1 474,780 - B158DRAFT_2313 0.24 -1.1 474,780 - B158DRAFT_2313 0.24 -0.6 474,780 - B158DRAFT_2313 0.24 -1.2 474,780 - B158DRAFT_2313 0.24 -0.7 474,791 - B158DRAFT_2313 0.25 -0.8 474,908 + B158DRAFT_2313 0.32 +0.2 474,916 - B158DRAFT_2313 0.33 +0.4 474,923 + B158DRAFT_2313 0.33 -0.8 474,961 - B158DRAFT_2313 0.36 -0.6 474,963 + B158DRAFT_2313 0.36 -1.1 474,963 + B158DRAFT_2313 0.36 -1.0 474,971 - B158DRAFT_2313 0.36 -0.7 474,976 - B158DRAFT_2313 0.37 +0.2 474,997 - B158DRAFT_2313 0.38 -1.4 475,025 - B158DRAFT_2313 0.40 -0.9 475,054 - B158DRAFT_2313 0.42 -0.7 475,093 + B158DRAFT_2313 0.44 -0.7 475,210 + B158DRAFT_2313 0.52 -0.9 475,210 + B158DRAFT_2313 0.52 -1.8 475,217 - B158DRAFT_2313 0.52 +0.2 475,218 - B158DRAFT_2313 0.52 -1.9 475,218 - B158DRAFT_2313 0.52 -0.2 475,218 - B158DRAFT_2313 0.52 -0.4 475,218 - B158DRAFT_2313 0.52 +0.4 475,218 - B158DRAFT_2313 0.52 +1.1 475,224 - B158DRAFT_2313 0.53 -1.2 475,236 - B158DRAFT_2313 0.53 -1.3 475,238 + B158DRAFT_2313 0.54 -2.3 475,283 - B158DRAFT_2313 0.56 +0.1 475,283 - B158DRAFT_2313 0.56 -0.7 475,297 + B158DRAFT_2313 0.57 -0.7 475,305 - B158DRAFT_2313 0.58 -0.6 475,305 - B158DRAFT_2313 0.58 -0.0 475,305 - B158DRAFT_2313 0.58 -0.4
Or see this region's nucleotide sequence