Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2263 and B158DRAFT_2264 are separated by 54 nucleotides B158DRAFT_2264 and B158DRAFT_2265 are separated by 104 nucleotides B158DRAFT_2265 and B158DRAFT_2266 are separated by 145 nucleotides
B158DRAFT_2263: B158DRAFT_2263 - tRNA (guanine-N1)-methyltransferase, at 426,418 to 427,185
_2263
B158DRAFT_2264: B158DRAFT_2264 - ribosomal protein L19, bacterial type, at 427,240 to 427,596
_2264
B158DRAFT_2265: B158DRAFT_2265 - tyrosine recombinase XerD, at 427,701 to 428,588
_2265
B158DRAFT_2266: B158DRAFT_2266 - Protein-disulfide isomerase, at 428,734 to 429,492
_2266
Position (kb)
427
428
429 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 427.736 kb on + strand at 427.744 kb on - strand at 427.744 kb on - strand at 427.744 kb on - strand at 427.754 kb on - strand at 427.759 kb on - strand at 427.759 kb on - strand at 427.989 kb on - strand, within B158DRAFT_2265 at 428.053 kb on - strand, within B158DRAFT_2265 at 428.082 kb on + strand, within B158DRAFT_2265 at 428.138 kb on - strand, within B158DRAFT_2265 at 428.187 kb on + strand, within B158DRAFT_2265 at 428.187 kb on + strand, within B158DRAFT_2265 at 428.210 kb on + strand, within B158DRAFT_2265 at 428.218 kb on - strand, within B158DRAFT_2265 at 428.218 kb on - strand, within B158DRAFT_2265 at 428.232 kb on + strand, within B158DRAFT_2265 at 428.239 kb on - strand, within B158DRAFT_2265 at 428.262 kb on + strand, within B158DRAFT_2265 at 428.263 kb on - strand, within B158DRAFT_2265 at 428.265 kb on - strand, within B158DRAFT_2265 at 428.275 kb on - strand, within B158DRAFT_2265 at 428.277 kb on - strand, within B158DRAFT_2265 at 428.282 kb on - strand, within B158DRAFT_2265 at 428.289 kb on + strand, within B158DRAFT_2265 at 428.329 kb on + strand, within B158DRAFT_2265 at 428.352 kb on + strand, within B158DRAFT_2265 at 428.365 kb on - strand, within B158DRAFT_2265 at 428.418 kb on + strand, within B158DRAFT_2265 at 428.426 kb on - strand, within B158DRAFT_2265 at 428.453 kb on + strand, within B158DRAFT_2265 at 428.453 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.490 kb on - strand, within B158DRAFT_2265 at 428.522 kb on + strand at 428.522 kb on + strand at 428.522 kb on + strand at 428.522 kb on + strand at 428.522 kb on + strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.556 kb on + strand at 428.607 kb on + strand at 428.801 kb on - strand at 428.868 kb on + strand, within B158DRAFT_2266 at 428.889 kb on + strand, within B158DRAFT_2266 at 429.070 kb on + strand, within B158DRAFT_2266 at 429.234 kb on - strand, within B158DRAFT_2266 at 429.252 kb on + strand, within B158DRAFT_2266 at 429.267 kb on - strand, within B158DRAFT_2266 at 429.352 kb on + strand, within B158DRAFT_2266 at 429.469 kb on - strand at 429.479 kb on - strand at 429.505 kb on - strand at 429.516 kb on - strand at 429.535 kb on + strand at 429.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 427,736 + -1.7 427,744 - -1.0 427,744 - +0.1 427,744 - -1.1 427,754 - -0.9 427,759 - -0.7 427,759 - -1.0 427,989 - B158DRAFT_2265 0.32 +0.8 428,053 - B158DRAFT_2265 0.40 -1.0 428,082 + B158DRAFT_2265 0.43 -1.2 428,138 - B158DRAFT_2265 0.49 -1.3 428,187 + B158DRAFT_2265 0.55 +0.7 428,187 + B158DRAFT_2265 0.55 +1.7 428,210 + B158DRAFT_2265 0.57 -0.9 428,218 - B158DRAFT_2265 0.58 -1.9 428,218 - B158DRAFT_2265 0.58 -0.9 428,232 + B158DRAFT_2265 0.60 -0.7 428,239 - B158DRAFT_2265 0.61 +0.4 428,262 + B158DRAFT_2265 0.63 -0.8 428,263 - B158DRAFT_2265 0.63 -0.6 428,265 - B158DRAFT_2265 0.64 +3.9 428,275 - B158DRAFT_2265 0.65 +3.1 428,277 - B158DRAFT_2265 0.65 -0.2 428,282 - B158DRAFT_2265 0.65 -1.5 428,289 + B158DRAFT_2265 0.66 -2.5 428,329 + B158DRAFT_2265 0.71 +0.5 428,352 + B158DRAFT_2265 0.73 -0.4 428,365 - B158DRAFT_2265 0.75 +0.4 428,418 + B158DRAFT_2265 0.81 -0.1 428,426 - B158DRAFT_2265 0.82 -0.7 428,453 + B158DRAFT_2265 0.85 -0.5 428,453 + B158DRAFT_2265 0.85 +0.6 428,471 + B158DRAFT_2265 0.87 -0.4 428,471 + B158DRAFT_2265 0.87 -1.0 428,471 + B158DRAFT_2265 0.87 -1.5 428,471 + B158DRAFT_2265 0.87 -1.2 428,471 + B158DRAFT_2265 0.87 +0.5 428,479 - B158DRAFT_2265 0.88 -0.3 428,479 - B158DRAFT_2265 0.88 -0.6 428,479 - B158DRAFT_2265 0.88 -0.7 428,479 - B158DRAFT_2265 0.88 -0.1 428,479 - B158DRAFT_2265 0.88 -1.1 428,490 - B158DRAFT_2265 0.89 -0.7 428,522 + +1.2 428,522 + -1.6 428,522 + -1.8 428,522 + -1.8 428,522 + +0.4 428,530 - -1.1 428,530 - +0.6 428,530 - +1.0 428,530 - -0.6 428,530 - -0.3 428,530 - +0.3 428,530 - +0.3 428,556 + -1.4 428,607 + +1.0 428,801 - +2.2 428,868 + B158DRAFT_2266 0.18 +1.5 428,889 + B158DRAFT_2266 0.20 +2.1 429,070 + B158DRAFT_2266 0.44 -0.0 429,234 - B158DRAFT_2266 0.66 +1.0 429,252 + B158DRAFT_2266 0.68 +3.0 429,267 - B158DRAFT_2266 0.70 +2.3 429,352 + B158DRAFT_2266 0.81 +1.5 429,469 - +0.5 429,479 - -0.6 429,505 - -2.3 429,516 - +0.2 429,535 + -1.2 429,538 - -1.4
Or see this region's nucleotide sequence