Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1177 and B158DRAFT_1178 are separated by 44 nucleotides B158DRAFT_1178 and B158DRAFT_1179 are separated by 116 nucleotides B158DRAFT_1179 and B158DRAFT_1180 are separated by 92 nucleotides
B158DRAFT_1177: B158DRAFT_1177 - hypothetical protein, at 1,216,115 to 1,217,236
_1177
B158DRAFT_1178: B158DRAFT_1178 - Sortase and related acyltransferases, at 1,217,281 to 1,217,772
_1178
B158DRAFT_1179: B158DRAFT_1179 - Uncharacterized protein conserved in bacteria, at 1,217,889 to 1,218,884
_1179
B158DRAFT_1180: B158DRAFT_1180 - Uncharacterized protein conserved in bacteria, at 1,218,977 to 1,220,689
_1180
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1216.981 kb on + strand, within B158DRAFT_1177 at 1216.994 kb on + strand, within B158DRAFT_1177 at 1217.002 kb on - strand, within B158DRAFT_1177 at 1217.002 kb on - strand, within B158DRAFT_1177 at 1217.002 kb on - strand, within B158DRAFT_1177 at 1217.012 kb on - strand, within B158DRAFT_1177 at 1217.012 kb on - strand, within B158DRAFT_1177 at 1217.012 kb on - strand, within B158DRAFT_1177 at 1217.022 kb on + strand, within B158DRAFT_1177 at 1217.022 kb on + strand, within B158DRAFT_1177 at 1217.022 kb on + strand, within B158DRAFT_1177 at 1217.039 kb on + strand, within B158DRAFT_1177 at 1217.039 kb on + strand, within B158DRAFT_1177 at 1217.044 kb on + strand, within B158DRAFT_1177 at 1217.061 kb on - strand, within B158DRAFT_1177 at 1217.082 kb on + strand, within B158DRAFT_1177 at 1217.123 kb on - strand, within B158DRAFT_1177 at 1217.128 kb on - strand at 1217.128 kb on - strand at 1217.166 kb on + strand at 1217.168 kb on - strand at 1217.196 kb on - strand at 1217.316 kb on - strand at 1217.321 kb on - strand at 1217.388 kb on + strand, within B158DRAFT_1178 at 1217.403 kb on - strand, within B158DRAFT_1178 at 1217.412 kb on + strand, within B158DRAFT_1178 at 1217.420 kb on - strand, within B158DRAFT_1178 at 1217.422 kb on + strand, within B158DRAFT_1178 at 1217.447 kb on + strand, within B158DRAFT_1178 at 1217.478 kb on + strand, within B158DRAFT_1178 at 1217.478 kb on + strand, within B158DRAFT_1178 at 1217.491 kb on + strand, within B158DRAFT_1178 at 1217.491 kb on + strand, within B158DRAFT_1178 at 1217.491 kb on + strand, within B158DRAFT_1178 at 1217.491 kb on + strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.499 kb on - strand, within B158DRAFT_1178 at 1217.529 kb on + strand, within B158DRAFT_1178 at 1217.529 kb on + strand, within B158DRAFT_1178 at 1217.542 kb on + strand, within B158DRAFT_1178 at 1217.581 kb on - strand, within B158DRAFT_1178 at 1217.616 kb on + strand, within B158DRAFT_1178 at 1217.623 kb on - strand, within B158DRAFT_1178 at 1217.643 kb on + strand, within B158DRAFT_1178 at 1217.659 kb on + strand, within B158DRAFT_1178 at 1217.756 kb on - strand at 1217.756 kb on - strand at 1217.864 kb on - strand at 1217.950 kb on + strand at 1217.950 kb on + strand at 1217.964 kb on - strand at 1217.965 kb on - strand at 1218.012 kb on - strand, within B158DRAFT_1179 at 1218.067 kb on + strand, within B158DRAFT_1179 at 1218.067 kb on + strand, within B158DRAFT_1179 at 1218.085 kb on - strand, within B158DRAFT_1179 at 1218.126 kb on + strand, within B158DRAFT_1179 at 1218.127 kb on + strand, within B158DRAFT_1179 at 1218.134 kb on + strand, within B158DRAFT_1179 at 1218.168 kb on + strand, within B158DRAFT_1179 at 1218.223 kb on + strand, within B158DRAFT_1179 at 1218.223 kb on + strand, within B158DRAFT_1179 at 1218.231 kb on - strand, within B158DRAFT_1179 at 1218.231 kb on - strand, within B158DRAFT_1179 at 1218.291 kb on - strand, within B158DRAFT_1179 at 1218.301 kb on + strand, within B158DRAFT_1179 at 1218.301 kb on + strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.309 kb on - strand, within B158DRAFT_1179 at 1218.314 kb on - strand, within B158DRAFT_1179 at 1218.326 kb on + strand, within B158DRAFT_1179 at 1218.498 kb on + strand, within B158DRAFT_1179 at 1218.530 kb on + strand, within B158DRAFT_1179 at 1218.549 kb on + strand, within B158DRAFT_1179 at 1218.557 kb on - strand, within B158DRAFT_1179 at 1218.557 kb on - strand, within B158DRAFT_1179 at 1218.557 kb on - strand, within B158DRAFT_1179 at 1218.613 kb on - strand, within B158DRAFT_1179 at 1218.639 kb on + strand, within B158DRAFT_1179 at 1218.691 kb on - strand, within B158DRAFT_1179 at 1218.746 kb on + strand, within B158DRAFT_1179 at 1218.766 kb on + strand, within B158DRAFT_1179 at 1218.766 kb on + strand, within B158DRAFT_1179 at 1218.766 kb on + strand, within B158DRAFT_1179 at 1218.766 kb on + strand, within B158DRAFT_1179 at 1218.767 kb on + strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.774 kb on - strand, within B158DRAFT_1179 at 1218.802 kb on - strand at 1218.815 kb on + strand at 1218.884 kb on + strand at 1218.892 kb on - strand at 1218.892 kb on - strand at 1218.893 kb on - strand at 1218.940 kb on - strand at 1218.940 kb on - strand at 1218.940 kb on - strand at 1218.940 kb on - strand at 1218.940 kb on - strand at 1218.961 kb on - strand at 1218.968 kb on - strand at 1218.982 kb on - strand at 1218.983 kb on - strand at 1218.985 kb on + strand at 1219.014 kb on + strand at 1219.014 kb on + strand at 1219.022 kb on - strand at 1219.022 kb on - strand at 1219.050 kb on + strand at 1219.050 kb on + strand at 1219.050 kb on + strand at 1219.058 kb on - strand at 1219.058 kb on - strand at 1219.083 kb on - strand at 1219.120 kb on + strand at 1219.123 kb on - strand at 1219.123 kb on - strand at 1219.128 kb on - strand at 1219.128 kb on - strand at 1219.128 kb on - strand at 1219.208 kb on - strand, within B158DRAFT_1180 at 1219.298 kb on + strand, within B158DRAFT_1180 at 1219.329 kb on + strand, within B158DRAFT_1180 at 1219.458 kb on + strand, within B158DRAFT_1180 at 1219.466 kb on - strand, within B158DRAFT_1180 at 1219.532 kb on + strand, within B158DRAFT_1180 at 1219.532 kb on + strand, within B158DRAFT_1180 at 1219.534 kb on - strand, within B158DRAFT_1180 at 1219.540 kb on - strand, within B158DRAFT_1180 at 1219.540 kb on - strand, within B158DRAFT_1180 at 1219.542 kb on - strand, within B158DRAFT_1180 at 1219.542 kb on - strand, within B158DRAFT_1180 at 1219.592 kb on + strand, within B158DRAFT_1180 at 1219.660 kb on + strand, within B158DRAFT_1180 at 1219.660 kb on + strand, within B158DRAFT_1180 at 1219.660 kb on + strand, within B158DRAFT_1180 at 1219.668 kb on - strand, within B158DRAFT_1180 at 1219.668 kb on - strand, within B158DRAFT_1180 at 1219.675 kb on - strand, within B158DRAFT_1180 at 1219.716 kb on - strand, within B158DRAFT_1180 at 1219.765 kb on + strand, within B158DRAFT_1180 at 1219.873 kb on + strand, within B158DRAFT_1180
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 1,216,981 + B158DRAFT_1177 0.77 -1.0 1,216,994 + B158DRAFT_1177 0.78 -0.6 1,217,002 - B158DRAFT_1177 0.79 +0.2 1,217,002 - B158DRAFT_1177 0.79 -0.1 1,217,002 - B158DRAFT_1177 0.79 -1.4 1,217,012 - B158DRAFT_1177 0.80 -0.5 1,217,012 - B158DRAFT_1177 0.80 -1.8 1,217,012 - B158DRAFT_1177 0.80 -0.4 1,217,022 + B158DRAFT_1177 0.81 +0.1 1,217,022 + B158DRAFT_1177 0.81 -2.3 1,217,022 + B158DRAFT_1177 0.81 -1.6 1,217,039 + B158DRAFT_1177 0.82 -0.7 1,217,039 + B158DRAFT_1177 0.82 -1.3 1,217,044 + B158DRAFT_1177 0.83 +0.2 1,217,061 - B158DRAFT_1177 0.84 -0.1 1,217,082 + B158DRAFT_1177 0.86 +0.7 1,217,123 - B158DRAFT_1177 0.90 -0.2 1,217,128 - -0.7 1,217,128 - -0.1 1,217,166 + -0.4 1,217,168 - -0.8 1,217,196 - +0.0 1,217,316 - +0.0 1,217,321 - -1.5 1,217,388 + B158DRAFT_1178 0.22 +1.0 1,217,403 - B158DRAFT_1178 0.25 +1.4 1,217,412 + B158DRAFT_1178 0.27 +1.2 1,217,420 - B158DRAFT_1178 0.28 -0.4 1,217,422 + B158DRAFT_1178 0.29 -1.1 1,217,447 + B158DRAFT_1178 0.34 -0.5 1,217,478 + B158DRAFT_1178 0.40 +1.0 1,217,478 + B158DRAFT_1178 0.40 +0.0 1,217,491 + B158DRAFT_1178 0.43 +0.7 1,217,491 + B158DRAFT_1178 0.43 -1.2 1,217,491 + B158DRAFT_1178 0.43 -0.6 1,217,491 + B158DRAFT_1178 0.43 -0.7 1,217,499 - B158DRAFT_1178 0.44 -0.4 1,217,499 - B158DRAFT_1178 0.44 -0.3 1,217,499 - B158DRAFT_1178 0.44 -2.2 1,217,499 - B158DRAFT_1178 0.44 -0.6 1,217,499 - B158DRAFT_1178 0.44 -0.4 1,217,499 - B158DRAFT_1178 0.44 +0.9 1,217,499 - B158DRAFT_1178 0.44 +0.3 1,217,499 - B158DRAFT_1178 0.44 -1.2 1,217,529 + B158DRAFT_1178 0.50 -0.5 1,217,529 + B158DRAFT_1178 0.50 +0.3 1,217,542 + B158DRAFT_1178 0.53 -1.7 1,217,581 - B158DRAFT_1178 0.61 +3.2 1,217,616 + B158DRAFT_1178 0.68 -0.1 1,217,623 - B158DRAFT_1178 0.70 -0.7 1,217,643 + B158DRAFT_1178 0.74 -2.1 1,217,659 + B158DRAFT_1178 0.77 -0.3 1,217,756 - +0.7 1,217,756 - -0.1 1,217,864 - -0.4 1,217,950 + -1.5 1,217,950 + -0.5 1,217,964 - -0.7 1,217,965 - +0.7 1,218,012 - B158DRAFT_1179 0.12 +1.5 1,218,067 + B158DRAFT_1179 0.18 -1.7 1,218,067 + B158DRAFT_1179 0.18 -0.8 1,218,085 - B158DRAFT_1179 0.20 -1.0 1,218,126 + B158DRAFT_1179 0.24 -1.6 1,218,127 + B158DRAFT_1179 0.24 +0.1 1,218,134 + B158DRAFT_1179 0.25 -0.0 1,218,168 + B158DRAFT_1179 0.28 -1.2 1,218,223 + B158DRAFT_1179 0.34 +3.0 1,218,223 + B158DRAFT_1179 0.34 -2.9 1,218,231 - B158DRAFT_1179 0.34 -0.3 1,218,231 - B158DRAFT_1179 0.34 -0.3 1,218,291 - B158DRAFT_1179 0.40 -0.6 1,218,301 + B158DRAFT_1179 0.41 -0.6 1,218,301 + B158DRAFT_1179 0.41 -0.6 1,218,309 - B158DRAFT_1179 0.42 -0.5 1,218,309 - B158DRAFT_1179 0.42 +0.6 1,218,309 - B158DRAFT_1179 0.42 -1.4 1,218,309 - B158DRAFT_1179 0.42 +0.7 1,218,309 - B158DRAFT_1179 0.42 -0.5 1,218,309 - B158DRAFT_1179 0.42 +4.0 1,218,309 - B158DRAFT_1179 0.42 +0.6 1,218,309 - B158DRAFT_1179 0.42 -0.6 1,218,314 - B158DRAFT_1179 0.43 -1.1 1,218,326 + B158DRAFT_1179 0.44 -1.0 1,218,498 + B158DRAFT_1179 0.61 +0.2 1,218,530 + B158DRAFT_1179 0.64 +0.0 1,218,549 + B158DRAFT_1179 0.66 +0.6 1,218,557 - B158DRAFT_1179 0.67 -3.0 1,218,557 - B158DRAFT_1179 0.67 -0.5 1,218,557 - B158DRAFT_1179 0.67 +0.4 1,218,613 - B158DRAFT_1179 0.73 -0.2 1,218,639 + B158DRAFT_1179 0.75 -0.3 1,218,691 - B158DRAFT_1179 0.81 -0.8 1,218,746 + B158DRAFT_1179 0.86 -0.5 1,218,766 + B158DRAFT_1179 0.88 -1.3 1,218,766 + B158DRAFT_1179 0.88 -0.9 1,218,766 + B158DRAFT_1179 0.88 +0.4 1,218,766 + B158DRAFT_1179 0.88 +0.0 1,218,767 + B158DRAFT_1179 0.88 -0.0 1,218,774 - B158DRAFT_1179 0.89 -0.9 1,218,774 - B158DRAFT_1179 0.89 +0.5 1,218,774 - B158DRAFT_1179 0.89 -1.0 1,218,774 - B158DRAFT_1179 0.89 -1.4 1,218,774 - B158DRAFT_1179 0.89 +0.6 1,218,774 - B158DRAFT_1179 0.89 -1.6 1,218,774 - B158DRAFT_1179 0.89 -1.1 1,218,774 - B158DRAFT_1179 0.89 +1.0 1,218,774 - B158DRAFT_1179 0.89 -1.6 1,218,802 - -0.2 1,218,815 + -1.1 1,218,884 + -0.2 1,218,892 - -1.2 1,218,892 - -0.4 1,218,893 - -0.2 1,218,940 - -0.3 1,218,940 - -0.5 1,218,940 - -0.5 1,218,940 - +0.4 1,218,940 - -1.3 1,218,961 - -1.9 1,218,968 - -0.7 1,218,982 - +0.8 1,218,983 - -1.1 1,218,985 + -0.2 1,219,014 + +0.4 1,219,014 + -0.2 1,219,022 - -1.5 1,219,022 - -0.3 1,219,050 + +0.2 1,219,050 + -0.8 1,219,050 + +1.1 1,219,058 - -0.6 1,219,058 - +0.5 1,219,083 - -1.5 1,219,120 + -0.8 1,219,123 - +0.4 1,219,123 - -0.6 1,219,128 - -0.3 1,219,128 - -1.2 1,219,128 - -0.5 1,219,208 - B158DRAFT_1180 0.13 -0.9 1,219,298 + B158DRAFT_1180 0.19 +0.0 1,219,329 + B158DRAFT_1180 0.21 +0.5 1,219,458 + B158DRAFT_1180 0.28 -0.7 1,219,466 - B158DRAFT_1180 0.29 -0.1 1,219,532 + B158DRAFT_1180 0.32 -0.1 1,219,532 + B158DRAFT_1180 0.32 -0.2 1,219,534 - B158DRAFT_1180 0.33 -0.2 1,219,540 - B158DRAFT_1180 0.33 -0.8 1,219,540 - B158DRAFT_1180 0.33 -0.2 1,219,542 - B158DRAFT_1180 0.33 +0.3 1,219,542 - B158DRAFT_1180 0.33 -2.3 1,219,592 + B158DRAFT_1180 0.36 -0.2 1,219,660 + B158DRAFT_1180 0.40 -3.4 1,219,660 + B158DRAFT_1180 0.40 -1.1 1,219,660 + B158DRAFT_1180 0.40 -1.5 1,219,668 - B158DRAFT_1180 0.40 -0.9 1,219,668 - B158DRAFT_1180 0.40 -0.7 1,219,675 - B158DRAFT_1180 0.41 -0.7 1,219,716 - B158DRAFT_1180 0.43 -1.1 1,219,765 + B158DRAFT_1180 0.46 -1.0 1,219,873 + B158DRAFT_1180 0.52 -0.4
Or see this region's nucleotide sequence