Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1063 and B158DRAFT_1064 are separated by 17 nucleotides B158DRAFT_1064 and B158DRAFT_1065 are separated by 1 nucleotides B158DRAFT_1065 and B158DRAFT_1066 are separated by 57 nucleotides
B158DRAFT_1063: B158DRAFT_1063 - Ribonuclease HI, at 1,104,066 to 1,104,515
_1063
B158DRAFT_1064: B158DRAFT_1064 - DNA polymerase III, epsilon subunit, Proteobacterial, at 1,104,533 to 1,105,234
_1064
B158DRAFT_1065: B158DRAFT_1065 - Enoyl-CoA hydratase/carnithine racemase, at 1,105,236 to 1,105,976
_1065
B158DRAFT_1066: B158DRAFT_1066 - anion transporter, at 1,106,034 to 1,107,428
_1066
Position (kb)
1105
1106 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1104.492 kb on + strand at 1104.523 kb on + strand at 1104.575 kb on - strand at 1104.575 kb on - strand at 1104.606 kb on + strand, within B158DRAFT_1064 at 1104.617 kb on - strand, within B158DRAFT_1064 at 1104.688 kb on - strand, within B158DRAFT_1064 at 1104.690 kb on + strand, within B158DRAFT_1064 at 1104.780 kb on + strand, within B158DRAFT_1064 at 1104.780 kb on + strand, within B158DRAFT_1064 at 1104.780 kb on + strand, within B158DRAFT_1064 at 1104.780 kb on + strand, within B158DRAFT_1064 at 1104.788 kb on - strand, within B158DRAFT_1064 at 1104.884 kb on + strand, within B158DRAFT_1064 at 1104.884 kb on + strand, within B158DRAFT_1064 at 1104.884 kb on + strand, within B158DRAFT_1064 at 1104.892 kb on - strand, within B158DRAFT_1064 at 1104.899 kb on + strand, within B158DRAFT_1064 at 1104.902 kb on - strand, within B158DRAFT_1064 at 1104.904 kb on + strand, within B158DRAFT_1064 at 1104.912 kb on - strand, within B158DRAFT_1064 at 1104.912 kb on - strand, within B158DRAFT_1064 at 1104.912 kb on - strand, within B158DRAFT_1064 at 1104.926 kb on - strand, within B158DRAFT_1064 at 1104.936 kb on + strand, within B158DRAFT_1064 at 1104.949 kb on + strand, within B158DRAFT_1064 at 1104.957 kb on - strand, within B158DRAFT_1064 at 1105.054 kb on - strand, within B158DRAFT_1064 at 1105.055 kb on + strand, within B158DRAFT_1064 at 1105.063 kb on - strand, within B158DRAFT_1064 at 1105.063 kb on - strand, within B158DRAFT_1064 at 1105.064 kb on - strand, within B158DRAFT_1064 at 1105.133 kb on - strand, within B158DRAFT_1064 at 1105.229 kb on + strand at 1105.229 kb on + strand at 1105.237 kb on - strand at 1105.292 kb on - strand at 1105.399 kb on - strand, within B158DRAFT_1065 at 1105.399 kb on - strand, within B158DRAFT_1065 at 1105.399 kb on - strand, within B158DRAFT_1065 at 1105.502 kb on - strand, within B158DRAFT_1065 at 1105.504 kb on - strand, within B158DRAFT_1065 at 1105.504 kb on - strand, within B158DRAFT_1065 at 1105.627 kb on + strand, within B158DRAFT_1065 at 1105.627 kb on + strand, within B158DRAFT_1065 at 1105.635 kb on - strand, within B158DRAFT_1065 at 1105.639 kb on + strand, within B158DRAFT_1065 at 1105.642 kb on - strand, within B158DRAFT_1065 at 1105.680 kb on + strand, within B158DRAFT_1065 at 1105.685 kb on + strand, within B158DRAFT_1065 at 1105.688 kb on - strand, within B158DRAFT_1065 at 1105.688 kb on - strand, within B158DRAFT_1065 at 1105.751 kb on - strand, within B158DRAFT_1065 at 1105.807 kb on - strand, within B158DRAFT_1065 at 1106.031 kb on - strand at 1106.031 kb on - strand at 1106.077 kb on + strand at 1106.125 kb on - strand at 1106.125 kb on - strand at 1106.125 kb on - strand at 1106.186 kb on - strand, within B158DRAFT_1066 at 1106.254 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.402 kb on + strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.410 kb on - strand, within B158DRAFT_1066 at 1106.490 kb on + strand, within B158DRAFT_1066 at 1106.490 kb on + strand, within B158DRAFT_1066 at 1106.490 kb on + strand, within B158DRAFT_1066 at 1106.490 kb on + strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.498 kb on - strand, within B158DRAFT_1066 at 1106.539 kb on + strand, within B158DRAFT_1066 at 1106.547 kb on - strand, within B158DRAFT_1066 at 1106.581 kb on - strand, within B158DRAFT_1066 at 1106.586 kb on - strand, within B158DRAFT_1066 at 1106.632 kb on + strand, within B158DRAFT_1066 at 1106.641 kb on + strand, within B158DRAFT_1066 at 1106.641 kb on + strand, within B158DRAFT_1066 at 1106.641 kb on + strand, within B158DRAFT_1066 at 1106.641 kb on + strand, within B158DRAFT_1066 at 1106.641 kb on + strand, within B158DRAFT_1066 at 1106.649 kb on - strand, within B158DRAFT_1066 at 1106.649 kb on - strand, within B158DRAFT_1066 at 1106.649 kb on - strand, within B158DRAFT_1066 at 1106.649 kb on - strand, within B158DRAFT_1066 at 1106.671 kb on - strand, within B158DRAFT_1066 at 1106.706 kb on + strand, within B158DRAFT_1066 at 1106.706 kb on + strand, within B158DRAFT_1066 at 1106.706 kb on + strand, within B158DRAFT_1066 at 1106.714 kb on - strand, within B158DRAFT_1066 at 1106.731 kb on - strand, within B158DRAFT_1066 at 1106.735 kb on + strand, within B158DRAFT_1066 at 1106.743 kb on - strand, within B158DRAFT_1066 at 1106.753 kb on - strand, within B158DRAFT_1066 at 1106.753 kb on - strand, within B158DRAFT_1066 at 1106.753 kb on - strand, within B158DRAFT_1066 at 1106.787 kb on - strand, within B158DRAFT_1066 at 1106.860 kb on + strand, within B158DRAFT_1066 at 1106.908 kb on - strand, within B158DRAFT_1066 at 1106.909 kb on - strand, within B158DRAFT_1066 at 1106.949 kb on + strand, within B158DRAFT_1066 at 1106.959 kb on + strand, within B158DRAFT_1066 at 1106.963 kb on + strand, within B158DRAFT_1066
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 1,104,492 + -2.6 1,104,523 + +2.0 1,104,575 - +3.2 1,104,575 - +2.4 1,104,606 + B158DRAFT_1064 0.10 +3.6 1,104,617 - B158DRAFT_1064 0.12 +2.7 1,104,688 - B158DRAFT_1064 0.22 +0.8 1,104,690 + B158DRAFT_1064 0.22 +2.0 1,104,780 + B158DRAFT_1064 0.35 +2.1 1,104,780 + B158DRAFT_1064 0.35 +1.3 1,104,780 + B158DRAFT_1064 0.35 +2.3 1,104,780 + B158DRAFT_1064 0.35 +0.4 1,104,788 - B158DRAFT_1064 0.36 +3.0 1,104,884 + B158DRAFT_1064 0.50 +2.8 1,104,884 + B158DRAFT_1064 0.50 +3.9 1,104,884 + B158DRAFT_1064 0.50 +2.4 1,104,892 - B158DRAFT_1064 0.51 +1.9 1,104,899 + B158DRAFT_1064 0.52 +2.7 1,104,902 - B158DRAFT_1064 0.53 +2.1 1,104,904 + B158DRAFT_1064 0.53 +2.3 1,104,912 - B158DRAFT_1064 0.54 +2.9 1,104,912 - B158DRAFT_1064 0.54 +2.5 1,104,912 - B158DRAFT_1064 0.54 +2.8 1,104,926 - B158DRAFT_1064 0.56 +2.7 1,104,936 + B158DRAFT_1064 0.57 +2.2 1,104,949 + B158DRAFT_1064 0.59 -0.5 1,104,957 - B158DRAFT_1064 0.60 +3.1 1,105,054 - B158DRAFT_1064 0.74 +2.1 1,105,055 + B158DRAFT_1064 0.74 +2.6 1,105,063 - B158DRAFT_1064 0.75 +2.7 1,105,063 - B158DRAFT_1064 0.75 +2.8 1,105,064 - B158DRAFT_1064 0.76 +1.8 1,105,133 - B158DRAFT_1064 0.85 +2.4 1,105,229 + -0.9 1,105,229 + -1.3 1,105,237 - -1.0 1,105,292 - -1.2 1,105,399 - B158DRAFT_1065 0.22 -0.0 1,105,399 - B158DRAFT_1065 0.22 -0.1 1,105,399 - B158DRAFT_1065 0.22 -1.1 1,105,502 - B158DRAFT_1065 0.36 -0.0 1,105,504 - B158DRAFT_1065 0.36 +0.5 1,105,504 - B158DRAFT_1065 0.36 +0.6 1,105,627 + B158DRAFT_1065 0.53 -0.5 1,105,627 + B158DRAFT_1065 0.53 +0.2 1,105,635 - B158DRAFT_1065 0.54 -1.1 1,105,639 + B158DRAFT_1065 0.54 -0.2 1,105,642 - B158DRAFT_1065 0.55 -3.4 1,105,680 + B158DRAFT_1065 0.60 -1.4 1,105,685 + B158DRAFT_1065 0.61 +0.1 1,105,688 - B158DRAFT_1065 0.61 +0.0 1,105,688 - B158DRAFT_1065 0.61 -0.9 1,105,751 - B158DRAFT_1065 0.70 -0.1 1,105,807 - B158DRAFT_1065 0.77 -0.1 1,106,031 - -0.5 1,106,031 - -0.5 1,106,077 + +2.2 1,106,125 - -2.4 1,106,125 - -0.6 1,106,125 - -0.9 1,106,186 - B158DRAFT_1066 0.11 -1.5 1,106,254 + B158DRAFT_1066 0.16 -0.2 1,106,402 + B158DRAFT_1066 0.26 +0.9 1,106,402 + B158DRAFT_1066 0.26 +1.0 1,106,402 + B158DRAFT_1066 0.26 -0.3 1,106,402 + B158DRAFT_1066 0.26 -1.1 1,106,402 + B158DRAFT_1066 0.26 +0.2 1,106,402 + B158DRAFT_1066 0.26 -0.3 1,106,402 + B158DRAFT_1066 0.26 -0.9 1,106,410 - B158DRAFT_1066 0.27 -1.8 1,106,410 - B158DRAFT_1066 0.27 +0.7 1,106,410 - B158DRAFT_1066 0.27 -0.7 1,106,410 - B158DRAFT_1066 0.27 +1.9 1,106,410 - B158DRAFT_1066 0.27 +1.0 1,106,410 - B158DRAFT_1066 0.27 -0.6 1,106,410 - B158DRAFT_1066 0.27 -0.5 1,106,410 - B158DRAFT_1066 0.27 -1.8 1,106,410 - B158DRAFT_1066 0.27 +0.9 1,106,490 + B158DRAFT_1066 0.33 -0.9 1,106,490 + B158DRAFT_1066 0.33 +0.1 1,106,490 + B158DRAFT_1066 0.33 +0.2 1,106,490 + B158DRAFT_1066 0.33 +2.4 1,106,498 - B158DRAFT_1066 0.33 +0.8 1,106,498 - B158DRAFT_1066 0.33 -0.1 1,106,498 - B158DRAFT_1066 0.33 -0.3 1,106,498 - B158DRAFT_1066 0.33 +0.2 1,106,498 - B158DRAFT_1066 0.33 -0.2 1,106,498 - B158DRAFT_1066 0.33 -0.7 1,106,498 - B158DRAFT_1066 0.33 -1.6 1,106,539 + B158DRAFT_1066 0.36 +0.1 1,106,547 - B158DRAFT_1066 0.37 -0.6 1,106,581 - B158DRAFT_1066 0.39 -0.1 1,106,586 - B158DRAFT_1066 0.40 -1.2 1,106,632 + B158DRAFT_1066 0.43 +0.0 1,106,641 + B158DRAFT_1066 0.44 +0.7 1,106,641 + B158DRAFT_1066 0.44 -0.3 1,106,641 + B158DRAFT_1066 0.44 +0.3 1,106,641 + B158DRAFT_1066 0.44 +0.6 1,106,641 + B158DRAFT_1066 0.44 -0.8 1,106,649 - B158DRAFT_1066 0.44 +1.8 1,106,649 - B158DRAFT_1066 0.44 +0.2 1,106,649 - B158DRAFT_1066 0.44 +1.8 1,106,649 - B158DRAFT_1066 0.44 -0.5 1,106,671 - B158DRAFT_1066 0.46 -2.5 1,106,706 + B158DRAFT_1066 0.48 -1.1 1,106,706 + B158DRAFT_1066 0.48 -1.7 1,106,706 + B158DRAFT_1066 0.48 -0.9 1,106,714 - B158DRAFT_1066 0.49 +0.3 1,106,731 - B158DRAFT_1066 0.50 -0.6 1,106,735 + B158DRAFT_1066 0.50 -0.6 1,106,743 - B158DRAFT_1066 0.51 -0.4 1,106,753 - B158DRAFT_1066 0.52 -1.1 1,106,753 - B158DRAFT_1066 0.52 -1.1 1,106,753 - B158DRAFT_1066 0.52 -0.7 1,106,787 - B158DRAFT_1066 0.54 -0.7 1,106,860 + B158DRAFT_1066 0.59 -1.1 1,106,908 - B158DRAFT_1066 0.63 -0.3 1,106,909 - B158DRAFT_1066 0.63 -1.0 1,106,949 + B158DRAFT_1066 0.66 +0.7 1,106,959 + B158DRAFT_1066 0.66 -1.1 1,106,963 + B158DRAFT_1066 0.67 +1.1
Or see this region's nucleotide sequence