Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0873 and B158DRAFT_0874 are separated by 76 nucleotides B158DRAFT_0874 and B158DRAFT_0875 overlap by 4 nucleotides B158DRAFT_0875 and B158DRAFT_0876 are separated by 107 nucleotides B158DRAFT_0876 and B158DRAFT_0877 are separated by 422 nucleotides
B158DRAFT_0873: B158DRAFT_0873 - DNA adenine methylase Dam, at 888,996 to 889,829
_0873
B158DRAFT_0874: B158DRAFT_0874 - Protein of unknown function (DUF2970)., at 889,906 to 890,190
_0874
B158DRAFT_0875: B158DRAFT_0875 - hypothetical protein, at 890,187 to 890,501
_0875
B158DRAFT_0876: B158DRAFT_0876 - 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (EC 2.5.1.54), at 890,609 to 891,988
_0876
B158DRAFT_0877: B158DRAFT_0877 - DNA replication and repair protein RadA, at 892,411 to 893,778
_0877
Position (kb) 890
891
892 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 889.780 kb on + strand at 889.793 kb on - strand at 889.793 kb on - strand at 889.798 kb on - strand at 889.831 kb on - strand at 889.835 kb on + strand at 889.845 kb on - strand at 889.884 kb on + strand at 889.884 kb on + strand at 889.892 kb on - strand at 889.892 kb on - strand at 889.892 kb on - strand at 889.911 kb on + strand at 889.919 kb on - strand at 889.926 kb on + strand at 890.310 kb on + strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.398 kb on - strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.414 kb on - strand, within B158DRAFT_0875 at 890.414 kb on - strand, within B158DRAFT_0875 at 890.424 kb on + strand, within B158DRAFT_0875 at 890.424 kb on + strand, within B158DRAFT_0875 at 890.424 kb on + strand, within B158DRAFT_0875 at 890.432 kb on - strand, within B158DRAFT_0875 at 890.446 kb on - strand, within B158DRAFT_0875 at 890.446 kb on - strand, within B158DRAFT_0875 at 890.547 kb on + strand at 890.552 kb on + strand at 890.555 kb on - strand at 890.706 kb on - strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.730 kb on + strand at 890.730 kb on + strand at 890.730 kb on + strand at 890.731 kb on - strand at 890.731 kb on - strand at 890.731 kb on - strand at 890.738 kb on - strand at 890.755 kb on + strand, within B158DRAFT_0876 at 890.828 kb on + strand, within B158DRAFT_0876 at 890.829 kb on - strand, within B158DRAFT_0876 at 890.836 kb on - strand, within B158DRAFT_0876 at 890.876 kb on - strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.942 kb on + strand, within B158DRAFT_0876 at 890.945 kb on + strand, within B158DRAFT_0876 at 890.952 kb on + strand, within B158DRAFT_0876 at 890.967 kb on - strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.984 kb on - strand, within B158DRAFT_0876 at 890.984 kb on - strand, within B158DRAFT_0876 at 890.996 kb on + strand, within B158DRAFT_0876 at 890.996 kb on + strand, within B158DRAFT_0876 at 891.004 kb on - strand, within B158DRAFT_0876 at 891.008 kb on + strand, within B158DRAFT_0876 at 891.034 kb on + strand, within B158DRAFT_0876 at 891.089 kb on + strand, within B158DRAFT_0876 at 891.090 kb on - strand, within B158DRAFT_0876 at 891.123 kb on - strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.169 kb on + strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.177 kb on + strand, within B158DRAFT_0876 at 891.187 kb on + strand, within B158DRAFT_0876 at 891.187 kb on + strand, within B158DRAFT_0876 at 891.187 kb on + strand, within B158DRAFT_0876 at 891.193 kb on + strand, within B158DRAFT_0876 at 891.193 kb on - strand, within B158DRAFT_0876 at 891.245 kb on + strand, within B158DRAFT_0876 at 891.245 kb on + strand, within B158DRAFT_0876 at 891.245 kb on + strand, within B158DRAFT_0876 at 891.296 kb on - strand, within B158DRAFT_0876 at 891.303 kb on - strand, within B158DRAFT_0876 at 891.303 kb on - strand, within B158DRAFT_0876 at 891.312 kb on + strand, within B158DRAFT_0876 at 891.320 kb on - strand, within B158DRAFT_0876 at 891.327 kb on + strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.367 kb on + strand, within B158DRAFT_0876 at 891.367 kb on + strand, within B158DRAFT_0876 at 891.367 kb on + strand, within B158DRAFT_0876 at 891.375 kb on - strand, within B158DRAFT_0876 at 891.432 kb on + strand, within B158DRAFT_0876 at 891.437 kb on + strand, within B158DRAFT_0876 at 891.440 kb on - strand, within B158DRAFT_0876 at 891.456 kb on + strand, within B158DRAFT_0876 at 891.457 kb on - strand, within B158DRAFT_0876 at 891.506 kb on + strand, within B158DRAFT_0876 at 891.509 kb on - strand, within B158DRAFT_0876 at 891.509 kb on - strand, within B158DRAFT_0876 at 891.534 kb on - strand, within B158DRAFT_0876 at 891.629 kb on + strand, within B158DRAFT_0876 at 891.637 kb on - strand, within B158DRAFT_0876 at 891.638 kb on + strand, within B158DRAFT_0876 at 891.638 kb on + strand, within B158DRAFT_0876 at 891.646 kb on - strand, within B158DRAFT_0876 at 891.655 kb on + strand, within B158DRAFT_0876 at 891.673 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.716 kb on - strand, within B158DRAFT_0876 at 891.726 kb on - strand, within B158DRAFT_0876 at 891.788 kb on + strand, within B158DRAFT_0876 at 891.788 kb on + strand, within B158DRAFT_0876 at 891.794 kb on + strand, within B158DRAFT_0876 at 891.796 kb on - strand, within B158DRAFT_0876 at 891.844 kb on - strand, within B158DRAFT_0876 at 891.899 kb on + strand at 891.899 kb on + strand at 891.944 kb on - strand at 891.953 kb on + strand at 891.953 kb on + strand at 891.961 kb on - strand at 891.962 kb on + strand at 891.963 kb on + strand at 891.970 kb on - strand at 891.980 kb on - strand at 892.028 kb on - strand at 892.182 kb on + strand at 892.200 kb on - strand at 892.210 kb on - strand at 892.303 kb on + strand at 892.307 kb on + strand at 892.360 kb on - strand at 892.390 kb on - strand at 892.469 kb on + strand at 892.469 kb on + strand at 892.474 kb on + strand at 892.474 kb on + strand at 892.474 kb on + strand at 892.474 kb on + strand at 892.477 kb on - strand at 892.477 kb on - strand at 892.477 kb on - strand at 892.487 kb on - strand at 892.546 kb on + strand at 892.546 kb on + strand at 892.554 kb on - strand, within B158DRAFT_0877 at 892.576 kb on + strand, within B158DRAFT_0877 at 892.583 kb on + strand, within B158DRAFT_0877 at 892.584 kb on - strand, within B158DRAFT_0877 at 892.584 kb on - strand, within B158DRAFT_0877 at 892.589 kb on - strand, within B158DRAFT_0877 at 892.591 kb on - strand, within B158DRAFT_0877 at 892.593 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.616 kb on + strand, within B158DRAFT_0877 at 892.637 kb on + strand, within B158DRAFT_0877 at 892.645 kb on - strand, within B158DRAFT_0877 at 892.645 kb on - strand, within B158DRAFT_0877 at 892.663 kb on + strand, within B158DRAFT_0877 at 892.676 kb on + strand, within B158DRAFT_0877 at 892.748 kb on - strand, within B158DRAFT_0877 at 892.800 kb on - strand, within B158DRAFT_0877 at 892.810 kb on - strand, within B158DRAFT_0877 at 892.837 kb on + strand, within B158DRAFT_0877 at 892.921 kb on - strand, within B158DRAFT_0877 at 892.960 kb on + strand, within B158DRAFT_0877 at 892.972 kb on + strand, within B158DRAFT_0877 at 892.980 kb on - strand, within B158DRAFT_0877 at 892.980 kb on - strand, within B158DRAFT_0877 at 892.982 kb on + strand, within B158DRAFT_0877
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 889,780 + +9.0 889,793 - +8.5 889,793 - +8.9 889,798 - +7.9 889,831 - -0.1 889,835 + -0.6 889,845 - -1.1 889,884 + -1.1 889,884 + -1.2 889,892 - -0.2 889,892 - -1.2 889,892 - +0.6 889,911 + -0.8 889,919 - -0.4 889,926 + -0.0 890,310 + B158DRAFT_0875 0.39 +0.5 890,396 - B158DRAFT_0875 0.66 -0.4 890,396 - B158DRAFT_0875 0.66 -0.2 890,396 - B158DRAFT_0875 0.66 -0.3 890,396 - B158DRAFT_0875 0.66 +0.0 890,396 - B158DRAFT_0875 0.66 -0.7 890,398 - B158DRAFT_0875 0.67 -0.8 890,406 + B158DRAFT_0875 0.70 -0.2 890,406 + B158DRAFT_0875 0.70 -1.0 890,406 + B158DRAFT_0875 0.70 -0.6 890,406 + B158DRAFT_0875 0.70 -1.5 890,414 - B158DRAFT_0875 0.72 -0.1 890,414 - B158DRAFT_0875 0.72 -1.7 890,424 + B158DRAFT_0875 0.75 -0.5 890,424 + B158DRAFT_0875 0.75 -1.7 890,424 + B158DRAFT_0875 0.75 +0.6 890,432 - B158DRAFT_0875 0.78 -0.1 890,446 - B158DRAFT_0875 0.82 -2.9 890,446 - B158DRAFT_0875 0.82 -0.1 890,547 + -0.7 890,552 + -0.8 890,555 - -3.0 890,706 - -0.4 890,723 + -3.0 890,723 + -0.6 890,723 + -0.6 890,723 + -0.3 890,723 + -1.4 890,723 + +1.4 890,730 + -0.9 890,730 + +0.8 890,730 + -0.9 890,731 - +3.5 890,731 - -0.6 890,731 - -2.7 890,738 - +0.7 890,755 + B158DRAFT_0876 0.11 -2.3 890,828 + B158DRAFT_0876 0.16 -1.2 890,829 - B158DRAFT_0876 0.16 -0.1 890,836 - B158DRAFT_0876 0.16 -0.6 890,876 - B158DRAFT_0876 0.19 -1.0 890,902 + B158DRAFT_0876 0.21 -0.7 890,902 + B158DRAFT_0876 0.21 -2.7 890,902 + B158DRAFT_0876 0.21 -0.9 890,902 + B158DRAFT_0876 0.21 +0.3 890,902 + B158DRAFT_0876 0.21 -1.3 890,902 + B158DRAFT_0876 0.21 -2.1 890,902 + B158DRAFT_0876 0.21 -1.5 890,910 - B158DRAFT_0876 0.22 -1.9 890,910 - B158DRAFT_0876 0.22 -0.7 890,910 - B158DRAFT_0876 0.22 -1.9 890,910 - B158DRAFT_0876 0.22 +1.2 890,942 + B158DRAFT_0876 0.24 -1.3 890,945 + B158DRAFT_0876 0.24 +2.8 890,952 + B158DRAFT_0876 0.25 -0.6 890,967 - B158DRAFT_0876 0.26 -2.5 890,976 + B158DRAFT_0876 0.27 -0.6 890,976 + B158DRAFT_0876 0.27 -1.9 890,976 + B158DRAFT_0876 0.27 -1.1 890,976 + B158DRAFT_0876 0.27 -0.8 890,976 + B158DRAFT_0876 0.27 -0.7 890,976 + B158DRAFT_0876 0.27 -2.2 890,976 + B158DRAFT_0876 0.27 -1.1 890,976 + B158DRAFT_0876 0.27 -1.7 890,984 - B158DRAFT_0876 0.27 -1.3 890,984 - B158DRAFT_0876 0.27 +1.3 890,996 + B158DRAFT_0876 0.28 -1.0 890,996 + B158DRAFT_0876 0.28 +3.2 891,004 - B158DRAFT_0876 0.29 -3.1 891,008 + B158DRAFT_0876 0.29 -0.2 891,034 + B158DRAFT_0876 0.31 -1.0 891,089 + B158DRAFT_0876 0.35 -0.6 891,090 - B158DRAFT_0876 0.35 -0.2 891,123 - B158DRAFT_0876 0.37 -0.6 891,167 + B158DRAFT_0876 0.40 +1.4 891,167 + B158DRAFT_0876 0.40 +2.2 891,167 + B158DRAFT_0876 0.40 -1.6 891,167 + B158DRAFT_0876 0.40 +0.5 891,167 + B158DRAFT_0876 0.40 +1.1 891,167 + B158DRAFT_0876 0.40 -2.8 891,169 + B158DRAFT_0876 0.41 -2.2 891,175 - B158DRAFT_0876 0.41 -0.5 891,175 - B158DRAFT_0876 0.41 -0.4 891,175 - B158DRAFT_0876 0.41 -1.2 891,175 - B158DRAFT_0876 0.41 -0.5 891,175 - B158DRAFT_0876 0.41 -1.7 891,177 + B158DRAFT_0876 0.41 -1.3 891,187 + B158DRAFT_0876 0.42 -1.5 891,187 + B158DRAFT_0876 0.42 -3.4 891,187 + B158DRAFT_0876 0.42 -1.1 891,193 + B158DRAFT_0876 0.42 -1.0 891,193 - B158DRAFT_0876 0.42 -0.7 891,245 + B158DRAFT_0876 0.46 +0.9 891,245 + B158DRAFT_0876 0.46 -1.0 891,245 + B158DRAFT_0876 0.46 -3.1 891,296 - B158DRAFT_0876 0.50 -1.5 891,303 - B158DRAFT_0876 0.50 -0.9 891,303 - B158DRAFT_0876 0.50 -0.4 891,312 + B158DRAFT_0876 0.51 +2.0 891,320 - B158DRAFT_0876 0.52 +1.4 891,327 + B158DRAFT_0876 0.52 -0.9 891,335 - B158DRAFT_0876 0.53 +1.6 891,335 - B158DRAFT_0876 0.53 +1.4 891,335 - B158DRAFT_0876 0.53 -0.7 891,335 - B158DRAFT_0876 0.53 -1.5 891,367 + B158DRAFT_0876 0.55 -1.6 891,367 + B158DRAFT_0876 0.55 -0.8 891,367 + B158DRAFT_0876 0.55 -0.4 891,375 - B158DRAFT_0876 0.56 -0.9 891,432 + B158DRAFT_0876 0.60 +1.3 891,437 + B158DRAFT_0876 0.60 -2.3 891,440 - B158DRAFT_0876 0.60 -0.9 891,456 + B158DRAFT_0876 0.61 -0.5 891,457 - B158DRAFT_0876 0.61 -1.8 891,506 + B158DRAFT_0876 0.65 -1.6 891,509 - B158DRAFT_0876 0.65 -0.1 891,509 - B158DRAFT_0876 0.65 -0.3 891,534 - B158DRAFT_0876 0.67 +1.8 891,629 + B158DRAFT_0876 0.74 -0.9 891,637 - B158DRAFT_0876 0.74 -1.4 891,638 + B158DRAFT_0876 0.75 -1.7 891,638 + B158DRAFT_0876 0.75 +1.1 891,646 - B158DRAFT_0876 0.75 -2.1 891,655 + B158DRAFT_0876 0.76 +2.4 891,673 + B158DRAFT_0876 0.77 -0.5 891,700 + B158DRAFT_0876 0.79 -2.6 891,700 + B158DRAFT_0876 0.79 -0.8 891,700 + B158DRAFT_0876 0.79 -0.1 891,700 + B158DRAFT_0876 0.79 +0.6 891,700 + B158DRAFT_0876 0.79 -0.6 891,700 + B158DRAFT_0876 0.79 +2.1 891,700 + B158DRAFT_0876 0.79 -0.1 891,700 + B158DRAFT_0876 0.79 -1.5 891,708 - B158DRAFT_0876 0.80 -1.6 891,708 - B158DRAFT_0876 0.80 -0.3 891,708 - B158DRAFT_0876 0.80 -0.8 891,708 - B158DRAFT_0876 0.80 +0.1 891,708 - B158DRAFT_0876 0.80 -1.8 891,708 - B158DRAFT_0876 0.80 -0.6 891,708 - B158DRAFT_0876 0.80 +5.5 891,716 - B158DRAFT_0876 0.80 -1.5 891,726 - B158DRAFT_0876 0.81 +0.1 891,788 + B158DRAFT_0876 0.85 -0.6 891,788 + B158DRAFT_0876 0.85 -1.0 891,794 + B158DRAFT_0876 0.86 +2.0 891,796 - B158DRAFT_0876 0.86 -0.7 891,844 - B158DRAFT_0876 0.89 -0.8 891,899 + -0.8 891,899 + -0.8 891,944 - +0.9 891,953 + +0.2 891,953 + -0.1 891,961 - -3.0 891,962 + -1.2 891,963 + -0.9 891,970 - -1.0 891,980 - -1.4 892,028 - -1.6 892,182 + -1.5 892,200 - -0.8 892,210 - +0.9 892,303 + -0.5 892,307 + -1.7 892,360 - +0.4 892,390 - -2.4 892,469 + -0.9 892,469 + +0.2 892,474 + -0.8 892,474 + -0.1 892,474 + -0.3 892,474 + -1.0 892,477 - -0.1 892,477 - +1.4 892,477 - -1.0 892,487 - -1.5 892,546 + -1.1 892,546 + -1.2 892,554 - B158DRAFT_0877 0.10 +0.8 892,576 + B158DRAFT_0877 0.12 +0.0 892,583 + B158DRAFT_0877 0.13 -1.3 892,584 - B158DRAFT_0877 0.13 -0.6 892,584 - B158DRAFT_0877 0.13 -0.1 892,589 - B158DRAFT_0877 0.13 +0.5 892,591 - B158DRAFT_0877 0.13 -0.8 892,593 + B158DRAFT_0877 0.13 -0.5 892,607 + B158DRAFT_0877 0.14 -0.8 892,607 + B158DRAFT_0877 0.14 -0.9 892,607 + B158DRAFT_0877 0.14 -0.7 892,607 + B158DRAFT_0877 0.14 +0.9 892,607 + B158DRAFT_0877 0.14 -0.0 892,615 - B158DRAFT_0877 0.15 -0.4 892,615 - B158DRAFT_0877 0.15 -1.9 892,615 - B158DRAFT_0877 0.15 -0.4 892,615 - B158DRAFT_0877 0.15 +2.4 892,616 + B158DRAFT_0877 0.15 +0.1 892,637 + B158DRAFT_0877 0.17 +2.2 892,645 - B158DRAFT_0877 0.17 -0.8 892,645 - B158DRAFT_0877 0.17 -1.5 892,663 + B158DRAFT_0877 0.18 -2.4 892,676 + B158DRAFT_0877 0.19 +1.2 892,748 - B158DRAFT_0877 0.25 -0.6 892,800 - B158DRAFT_0877 0.28 -0.9 892,810 - B158DRAFT_0877 0.29 -1.6 892,837 + B158DRAFT_0877 0.31 +0.1 892,921 - B158DRAFT_0877 0.37 -1.4 892,960 + B158DRAFT_0877 0.40 -0.3 892,972 + B158DRAFT_0877 0.41 -0.5 892,980 - B158DRAFT_0877 0.42 -0.1 892,980 - B158DRAFT_0877 0.42 -1.1 892,982 + B158DRAFT_0877 0.42 +0.8
Or see this region's nucleotide sequence