Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0167 and B158DRAFT_0168 are separated by 92 nucleotides B158DRAFT_0168 and B158DRAFT_0169 are separated by 63 nucleotides B158DRAFT_0169 and B158DRAFT_0170 are separated by 11 nucleotides B158DRAFT_0170 and B158DRAFT_0171 are separated by 25 nucleotides
B158DRAFT_0167: B158DRAFT_0167 - phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 164,184 to 165,467
_0167
B158DRAFT_0168: B158DRAFT_0168 - Dicarboxylate transport., at 165,560 to 168,199
_0168
B158DRAFT_0169: B158DRAFT_0169 - hypothetical protein, at 168,263 to 168,457
_0169
B158DRAFT_0170: B158DRAFT_0170 - Uncharacterized protein conserved in bacteria, at 168,469 to 168,801
_0170
B158DRAFT_0171: B158DRAFT_0171 - Glutathione S-transferase, at 168,827 to 169,423
_0171
Position (kb)
165
166
167
168
169 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 165.439 kb on + strand at 165.470 kb on - strand at 165.496 kb on + strand at 165.496 kb on + strand at 165.496 kb on + strand at 165.504 kb on - strand at 165.509 kb on - strand at 165.552 kb on + strand at 165.552 kb on + strand at 165.587 kb on + strand at 165.587 kb on + strand at 165.595 kb on - strand at 165.595 kb on - strand at 165.595 kb on - strand at 165.602 kb on + strand at 165.602 kb on + strand at 165.608 kb on - strand at 165.642 kb on - strand at 165.643 kb on + strand at 165.645 kb on + strand at 165.770 kb on + strand at 165.770 kb on + strand at 165.770 kb on + strand at 165.770 kb on + strand at 165.770 kb on + strand at 165.770 kb on + strand at 165.778 kb on - strand at 165.778 kb on - strand at 165.778 kb on - strand at 165.805 kb on + strand at 165.805 kb on + strand at 165.813 kb on - strand at 165.813 kb on - strand at 165.813 kb on - strand at 165.813 kb on - strand at 165.813 kb on - strand at 165.918 kb on + strand, within B158DRAFT_0168 at 165.962 kb on - strand, within B158DRAFT_0168 at 165.986 kb on + strand, within B158DRAFT_0168 at 165.986 kb on + strand, within B158DRAFT_0168 at 165.986 kb on + strand, within B158DRAFT_0168 at 165.991 kb on + strand, within B158DRAFT_0168 at 165.991 kb on + strand, within B158DRAFT_0168 at 165.994 kb on - strand, within B158DRAFT_0168 at 165.994 kb on - strand, within B158DRAFT_0168 at 165.999 kb on - strand, within B158DRAFT_0168 at 165.999 kb on - strand, within B158DRAFT_0168 at 165.999 kb on - strand, within B158DRAFT_0168 at 166.015 kb on + strand, within B158DRAFT_0168 at 166.015 kb on + strand, within B158DRAFT_0168 at 166.023 kb on - strand, within B158DRAFT_0168 at 166.031 kb on - strand, within B158DRAFT_0168 at 166.089 kb on - strand, within B158DRAFT_0168 at 166.117 kb on - strand, within B158DRAFT_0168 at 166.284 kb on + strand, within B158DRAFT_0168 at 166.319 kb on + strand, within B158DRAFT_0168 at 166.374 kb on + strand, within B158DRAFT_0168 at 166.384 kb on + strand, within B158DRAFT_0168 at 166.395 kb on - strand, within B158DRAFT_0168 at 166.413 kb on + strand, within B158DRAFT_0168 at 166.413 kb on + strand, within B158DRAFT_0168 at 166.414 kb on - strand, within B158DRAFT_0168 at 166.420 kb on - strand, within B158DRAFT_0168 at 166.489 kb on + strand, within B158DRAFT_0168 at 166.573 kb on + strand, within B158DRAFT_0168 at 166.614 kb on + strand, within B158DRAFT_0168 at 166.622 kb on - strand, within B158DRAFT_0168 at 166.624 kb on - strand, within B158DRAFT_0168 at 166.672 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.682 kb on + strand, within B158DRAFT_0168 at 166.690 kb on - strand, within B158DRAFT_0168 at 166.690 kb on - strand, within B158DRAFT_0168 at 166.690 kb on - strand, within B158DRAFT_0168 at 166.755 kb on - strand, within B158DRAFT_0168 at 166.829 kb on + strand, within B158DRAFT_0168 at 167.030 kb on + strand, within B158DRAFT_0168 at 167.038 kb on + strand, within B158DRAFT_0168 at 167.038 kb on - strand, within B158DRAFT_0168 at 167.093 kb on + strand, within B158DRAFT_0168 at 167.093 kb on + strand, within B158DRAFT_0168 at 167.109 kb on + strand, within B158DRAFT_0168 at 167.258 kb on - strand, within B158DRAFT_0168 at 167.268 kb on + strand, within B158DRAFT_0168 at 167.268 kb on + strand, within B158DRAFT_0168 at 167.276 kb on - strand, within B158DRAFT_0168 at 167.276 kb on - strand, within B158DRAFT_0168 at 167.276 kb on - strand, within B158DRAFT_0168 at 167.362 kb on - strand, within B158DRAFT_0168 at 167.390 kb on + strand, within B158DRAFT_0168 at 167.507 kb on + strand, within B158DRAFT_0168 at 167.518 kb on + strand, within B158DRAFT_0168 at 167.531 kb on - strand, within B158DRAFT_0168 at 167.586 kb on + strand, within B158DRAFT_0168 at 167.586 kb on + strand, within B158DRAFT_0168 at 167.586 kb on + strand, within B158DRAFT_0168 at 167.586 kb on + strand, within B158DRAFT_0168 at 167.594 kb on - strand, within B158DRAFT_0168 at 167.594 kb on - strand, within B158DRAFT_0168 at 167.594 kb on - strand, within B158DRAFT_0168 at 167.594 kb on - strand, within B158DRAFT_0168 at 167.594 kb on - strand, within B158DRAFT_0168 at 167.594 kb on - strand, within B158DRAFT_0168 at 167.626 kb on + strand, within B158DRAFT_0168 at 167.634 kb on - strand, within B158DRAFT_0168 at 167.688 kb on + strand, within B158DRAFT_0168 at 167.727 kb on - strand, within B158DRAFT_0168 at 167.745 kb on + strand, within B158DRAFT_0168 at 167.796 kb on + strand, within B158DRAFT_0168 at 168.029 kb on + strand at 168.029 kb on - strand at 168.238 kb on + strand at 168.284 kb on + strand, within B158DRAFT_0169 at 168.307 kb on + strand, within B158DRAFT_0169 at 168.330 kb on - strand, within B158DRAFT_0169 at 168.421 kb on + strand, within B158DRAFT_0169 at 168.454 kb on - strand at 168.531 kb on - strand, within B158DRAFT_0170 at 168.535 kb on + strand, within B158DRAFT_0170 at 168.545 kb on + strand, within B158DRAFT_0170 at 168.553 kb on - strand, within B158DRAFT_0170 at 168.561 kb on - strand, within B158DRAFT_0170 at 168.567 kb on - strand, within B158DRAFT_0170 at 168.567 kb on - strand, within B158DRAFT_0170 at 168.573 kb on - strand, within B158DRAFT_0170 at 168.640 kb on + strand, within B158DRAFT_0170 at 168.735 kb on + strand, within B158DRAFT_0170 at 168.735 kb on + strand, within B158DRAFT_0170 at 168.743 kb on - strand, within B158DRAFT_0170 at 168.839 kb on - strand at 168.934 kb on + strand, within B158DRAFT_0171 at 168.944 kb on + strand, within B158DRAFT_0171 at 168.944 kb on + strand, within B158DRAFT_0171 at 168.944 kb on + strand, within B158DRAFT_0171 at 168.944 kb on + strand, within B158DRAFT_0171 at 168.944 kb on + strand, within B158DRAFT_0171 at 168.944 kb on + strand, within B158DRAFT_0171 at 168.952 kb on - strand, within B158DRAFT_0171 at 168.952 kb on - strand, within B158DRAFT_0171 at 168.952 kb on - strand, within B158DRAFT_0171 at 168.952 kb on - strand, within B158DRAFT_0171 at 168.952 kb on - strand, within B158DRAFT_0171 at 168.975 kb on + strand, within B158DRAFT_0171 at 168.997 kb on + strand, within B158DRAFT_0171 at 168.997 kb on + strand, within B158DRAFT_0171 at 168.997 kb on + strand, within B158DRAFT_0171 at 168.997 kb on + strand, within B158DRAFT_0171 at 169.005 kb on - strand, within B158DRAFT_0171 at 169.005 kb on - strand, within B158DRAFT_0171 at 169.083 kb on - strand, within B158DRAFT_0171 at 169.083 kb on - strand, within B158DRAFT_0171 at 169.083 kb on - strand, within B158DRAFT_0171 at 169.113 kb on + strand, within B158DRAFT_0171 at 169.114 kb on - strand, within B158DRAFT_0171 at 169.141 kb on + strand, within B158DRAFT_0171
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 165,439 + +1.0 165,470 - -0.3 165,496 + -1.4 165,496 + +0.4 165,496 + +0.1 165,504 - -0.5 165,509 - -0.8 165,552 + -1.5 165,552 + -2.8 165,587 + -0.8 165,587 + -0.8 165,595 - -0.5 165,595 - -1.3 165,595 - +0.3 165,602 + +1.0 165,602 + -0.8 165,608 - -1.4 165,642 - -2.0 165,643 + +0.4 165,645 + -0.3 165,770 + +0.0 165,770 + -1.3 165,770 + -0.1 165,770 + -1.3 165,770 + -2.2 165,770 + -2.0 165,778 - -0.2 165,778 - +0.7 165,778 - +1.0 165,805 + +2.1 165,805 + +1.0 165,813 - -0.8 165,813 - -0.9 165,813 - -0.5 165,813 - +0.4 165,813 - -0.1 165,918 + B158DRAFT_0168 0.14 -0.4 165,962 - B158DRAFT_0168 0.15 -1.2 165,986 + B158DRAFT_0168 0.16 -0.4 165,986 + B158DRAFT_0168 0.16 -1.7 165,986 + B158DRAFT_0168 0.16 -0.1 165,991 + B158DRAFT_0168 0.16 +0.4 165,991 + B158DRAFT_0168 0.16 +1.8 165,994 - B158DRAFT_0168 0.16 -0.1 165,994 - B158DRAFT_0168 0.16 -0.7 165,999 - B158DRAFT_0168 0.17 -2.0 165,999 - B158DRAFT_0168 0.17 -1.0 165,999 - B158DRAFT_0168 0.17 -0.6 166,015 + B158DRAFT_0168 0.17 -0.1 166,015 + B158DRAFT_0168 0.17 -2.3 166,023 - B158DRAFT_0168 0.18 +0.7 166,031 - B158DRAFT_0168 0.18 +0.6 166,089 - B158DRAFT_0168 0.20 +0.3 166,117 - B158DRAFT_0168 0.21 -0.2 166,284 + B158DRAFT_0168 0.27 -2.7 166,319 + B158DRAFT_0168 0.29 +1.1 166,374 + B158DRAFT_0168 0.31 -2.2 166,384 + B158DRAFT_0168 0.31 -0.1 166,395 - B158DRAFT_0168 0.32 -1.8 166,413 + B158DRAFT_0168 0.32 -1.1 166,413 + B158DRAFT_0168 0.32 +4.0 166,414 - B158DRAFT_0168 0.32 +1.6 166,420 - B158DRAFT_0168 0.33 -0.2 166,489 + B158DRAFT_0168 0.35 +0.2 166,573 + B158DRAFT_0168 0.38 -1.3 166,614 + B158DRAFT_0168 0.40 -0.0 166,622 - B158DRAFT_0168 0.40 -1.3 166,624 - B158DRAFT_0168 0.40 -1.7 166,672 + B158DRAFT_0168 0.42 -1.1 166,682 + B158DRAFT_0168 0.42 -1.6 166,682 + B158DRAFT_0168 0.42 -0.9 166,682 + B158DRAFT_0168 0.42 -0.6 166,682 + B158DRAFT_0168 0.42 -0.0 166,682 + B158DRAFT_0168 0.42 -1.2 166,682 + B158DRAFT_0168 0.42 +0.2 166,682 + B158DRAFT_0168 0.42 -0.2 166,690 - B158DRAFT_0168 0.43 +1.1 166,690 - B158DRAFT_0168 0.43 +0.9 166,690 - B158DRAFT_0168 0.43 -1.0 166,755 - B158DRAFT_0168 0.45 -0.7 166,829 + B158DRAFT_0168 0.48 +0.4 167,030 + B158DRAFT_0168 0.56 -0.3 167,038 + B158DRAFT_0168 0.56 +5.3 167,038 - B158DRAFT_0168 0.56 +0.3 167,093 + B158DRAFT_0168 0.58 -0.3 167,093 + B158DRAFT_0168 0.58 +0.7 167,109 + B158DRAFT_0168 0.59 +0.5 167,258 - B158DRAFT_0168 0.64 -1.6 167,268 + B158DRAFT_0168 0.65 -0.4 167,268 + B158DRAFT_0168 0.65 +0.3 167,276 - B158DRAFT_0168 0.65 +0.7 167,276 - B158DRAFT_0168 0.65 -0.5 167,276 - B158DRAFT_0168 0.65 -1.8 167,362 - B158DRAFT_0168 0.68 -0.5 167,390 + B158DRAFT_0168 0.69 -1.0 167,507 + B158DRAFT_0168 0.74 +0.8 167,518 + B158DRAFT_0168 0.74 +0.9 167,531 - B158DRAFT_0168 0.75 +1.7 167,586 + B158DRAFT_0168 0.77 -1.3 167,586 + B158DRAFT_0168 0.77 +0.1 167,586 + B158DRAFT_0168 0.77 +1.7 167,586 + B158DRAFT_0168 0.77 -0.6 167,594 - B158DRAFT_0168 0.77 +2.3 167,594 - B158DRAFT_0168 0.77 +0.4 167,594 - B158DRAFT_0168 0.77 -0.6 167,594 - B158DRAFT_0168 0.77 -2.2 167,594 - B158DRAFT_0168 0.77 +2.3 167,594 - B158DRAFT_0168 0.77 -0.4 167,626 + B158DRAFT_0168 0.78 -0.6 167,634 - B158DRAFT_0168 0.79 -0.5 167,688 + B158DRAFT_0168 0.81 -0.2 167,727 - B158DRAFT_0168 0.82 -0.3 167,745 + B158DRAFT_0168 0.83 +0.2 167,796 + B158DRAFT_0168 0.85 -0.8 168,029 + -0.6 168,029 - -0.8 168,238 + +0.1 168,284 + B158DRAFT_0169 0.11 +0.0 168,307 + B158DRAFT_0169 0.23 +0.1 168,330 - B158DRAFT_0169 0.34 -0.6 168,421 + B158DRAFT_0169 0.81 -0.5 168,454 - -0.8 168,531 - B158DRAFT_0170 0.19 -0.8 168,535 + B158DRAFT_0170 0.20 -0.4 168,545 + B158DRAFT_0170 0.23 +0.2 168,553 - B158DRAFT_0170 0.25 +3.4 168,561 - B158DRAFT_0170 0.28 -0.3 168,567 - B158DRAFT_0170 0.29 +0.2 168,567 - B158DRAFT_0170 0.29 -0.0 168,573 - B158DRAFT_0170 0.31 +1.8 168,640 + B158DRAFT_0170 0.51 +1.2 168,735 + B158DRAFT_0170 0.80 +0.3 168,735 + B158DRAFT_0170 0.80 -0.6 168,743 - B158DRAFT_0170 0.82 +0.3 168,839 - -0.3 168,934 + B158DRAFT_0171 0.18 -0.8 168,944 + B158DRAFT_0171 0.20 +0.4 168,944 + B158DRAFT_0171 0.20 -0.7 168,944 + B158DRAFT_0171 0.20 -0.2 168,944 + B158DRAFT_0171 0.20 -1.4 168,944 + B158DRAFT_0171 0.20 +1.8 168,944 + B158DRAFT_0171 0.20 -0.9 168,952 - B158DRAFT_0171 0.21 -1.9 168,952 - B158DRAFT_0171 0.21 +0.4 168,952 - B158DRAFT_0171 0.21 +0.3 168,952 - B158DRAFT_0171 0.21 -1.6 168,952 - B158DRAFT_0171 0.21 -0.1 168,975 + B158DRAFT_0171 0.25 -1.4 168,997 + B158DRAFT_0171 0.28 -0.3 168,997 + B158DRAFT_0171 0.28 -0.7 168,997 + B158DRAFT_0171 0.28 -0.4 168,997 + B158DRAFT_0171 0.28 +2.8 169,005 - B158DRAFT_0171 0.30 +1.2 169,005 - B158DRAFT_0171 0.30 +1.8 169,083 - B158DRAFT_0171 0.43 -0.6 169,083 - B158DRAFT_0171 0.43 +0.8 169,083 - B158DRAFT_0171 0.43 -1.9 169,113 + B158DRAFT_0171 0.48 -0.5 169,114 - B158DRAFT_0171 0.48 -0.3 169,141 + B158DRAFT_0171 0.53 -1.0
Or see this region's nucleotide sequence