Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_3954

Experiment: D-Cycloserine 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_3952 and PfGW456L13_3953 are separated by 231 nucleotidesPfGW456L13_3953 and PfGW456L13_3954 are separated by 49 nucleotidesPfGW456L13_3954 and PfGW456L13_3955 are separated by 29 nucleotidesPfGW456L13_3955 and PfGW456L13_5223 are separated by 197 nucleotidesPfGW456L13_5223 and PfGW456L13_5224 are separated by 83 nucleotides PfGW456L13_3952 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 4,433,660 to 4,434,589 _3952 PfGW456L13_3953 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 4,434,821 to 4,435,258 _3953 PfGW456L13_3954 - Probable hydrolase, at 4,435,308 to 4,436,141 _3954 PfGW456L13_3955 - Transcriptional regulator, TetR family, at 4,436,171 to 4,436,710 _3955 PfGW456L13_5223 - tRNA-Glu-TTC, at 4,436,908 to 4,436,980 _5223 PfGW456L13_5224 - tRNA-Ala-GGC, at 4,437,064 to 4,437,136 _5224 Position (kb) 4435 4436 4437Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4434.486 kb on + strand, within PfGW456L13_3952at 4434.487 kb on - strand, within PfGW456L13_3952at 4434.487 kb on - strand, within PfGW456L13_3952at 4434.545 kb on - strandat 4434.654 kb on + strandat 4434.661 kb on + strandat 4434.684 kb on + strandat 4434.685 kb on - strandat 4434.685 kb on - strandat 4434.747 kb on + strandat 4434.747 kb on + strandat 4434.747 kb on + strandat 4434.932 kb on + strand, within PfGW456L13_3953at 4434.982 kb on + strand, within PfGW456L13_3953at 4434.983 kb on - strand, within PfGW456L13_3953at 4435.038 kb on + strand, within PfGW456L13_3953at 4435.038 kb on + strand, within PfGW456L13_3953at 4435.038 kb on + strand, within PfGW456L13_3953at 4435.039 kb on - strand, within PfGW456L13_3953at 4435.039 kb on - strand, within PfGW456L13_3953at 4435.167 kb on + strand, within PfGW456L13_3953at 4435.167 kb on + strand, within PfGW456L13_3953at 4435.167 kb on + strand, within PfGW456L13_3953at 4435.168 kb on - strand, within PfGW456L13_3953at 4435.168 kb on - strand, within PfGW456L13_3953at 4435.168 kb on - strand, within PfGW456L13_3953at 4435.278 kb on + strandat 4435.278 kb on + strandat 4435.279 kb on - strandat 4435.299 kb on - strandat 4435.299 kb on - strandat 4435.299 kb on - strandat 4435.353 kb on + strandat 4435.353 kb on + strandat 4435.353 kb on + strandat 4435.353 kb on + strandat 4435.522 kb on + strand, within PfGW456L13_3954at 4435.522 kb on + strand, within PfGW456L13_3954at 4435.936 kb on - strand, within PfGW456L13_3954at 4435.936 kb on - strand, within PfGW456L13_3954at 4435.936 kb on - strand, within PfGW456L13_3954at 4436.130 kb on + strandat 4436.130 kb on + strandat 4436.131 kb on - strandat 4436.442 kb on + strand, within PfGW456L13_3955at 4436.442 kb on + strand, within PfGW456L13_3955at 4436.442 kb on + strand, within PfGW456L13_3955at 4436.443 kb on - strand, within PfGW456L13_3955at 4436.443 kb on - strand, within PfGW456L13_3955at 4436.443 kb on - strand, within PfGW456L13_3955at 4436.443 kb on - strand, within PfGW456L13_3955at 4436.577 kb on + strand, within PfGW456L13_3955at 4436.578 kb on - strand, within PfGW456L13_3955at 4436.702 kb on - strandat 4436.865 kb on - strandat 4436.882 kb on - strandat 4436.882 kb on - strandat 4436.882 kb on - strandat 4436.882 kb on - strandat 4437.129 kb on - strandat 4437.129 kb on - strandat 4437.129 kb on - strandat 4437.129 kb on - strandat 4437.129 kb on - strandat 4437.129 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.125 mg/ml
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4,434,486 + PfGW456L13_3952 0.89 +0.4
4,434,487 - PfGW456L13_3952 0.89 +1.8
4,434,487 - PfGW456L13_3952 0.89 +0.7
4,434,545 - -0.5
4,434,654 + +2.7
4,434,661 + -0.8
4,434,684 + -0.7
4,434,685 - -0.6
4,434,685 - +0.2
4,434,747 + -2.3
4,434,747 + -0.7
4,434,747 + +0.6
4,434,932 + PfGW456L13_3953 0.25 -0.3
4,434,982 + PfGW456L13_3953 0.37 -0.2
4,434,983 - PfGW456L13_3953 0.37 +1.0
4,435,038 + PfGW456L13_3953 0.50 +0.1
4,435,038 + PfGW456L13_3953 0.50 -0.2
4,435,038 + PfGW456L13_3953 0.50 -0.8
4,435,039 - PfGW456L13_3953 0.50 -0.0
4,435,039 - PfGW456L13_3953 0.50 +1.6
4,435,167 + PfGW456L13_3953 0.79 -0.2
4,435,167 + PfGW456L13_3953 0.79 +0.7
4,435,167 + PfGW456L13_3953 0.79 +1.0
4,435,168 - PfGW456L13_3953 0.79 -0.8
4,435,168 - PfGW456L13_3953 0.79 -0.3
4,435,168 - PfGW456L13_3953 0.79 -1.7
4,435,278 + -0.8
4,435,278 + -0.2
4,435,279 - +0.9
4,435,299 - +1.1
4,435,299 - -0.3
4,435,299 - +0.5
4,435,353 + -3.6
4,435,353 + -0.2
4,435,353 + -0.5
4,435,353 + +0.7
4,435,522 + PfGW456L13_3954 0.26 -0.2
4,435,522 + PfGW456L13_3954 0.26 +0.7
4,435,936 - PfGW456L13_3954 0.75 +0.8
4,435,936 - PfGW456L13_3954 0.75 +0.7
4,435,936 - PfGW456L13_3954 0.75 +0.0
4,436,130 + +1.2
4,436,130 + -0.6
4,436,131 - +0.3
4,436,442 + PfGW456L13_3955 0.50 +1.6
4,436,442 + PfGW456L13_3955 0.50 +0.3
4,436,442 + PfGW456L13_3955 0.50 -0.4
4,436,443 - PfGW456L13_3955 0.50 +0.2
4,436,443 - PfGW456L13_3955 0.50 +0.5
4,436,443 - PfGW456L13_3955 0.50 +0.4
4,436,443 - PfGW456L13_3955 0.50 -1.0
4,436,577 + PfGW456L13_3955 0.75 +0.7
4,436,578 - PfGW456L13_3955 0.75 -0.6
4,436,702 - +0.1
4,436,865 - +0.4
4,436,882 - -0.1
4,436,882 - +0.6
4,436,882 - +1.4
4,436,882 - +1.4
4,437,129 - -0.6
4,437,129 - -0.5
4,437,129 - -0.0
4,437,129 - +0.3
4,437,129 - -0.9
4,437,129 - -0.8

Or see this region's nucleotide sequence