Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0489 and Atu0490 are separated by 135 nucleotides Atu0490 and Atu0491 are separated by 82 nucleotides Atu0491 and Atu0492 are separated by 26 nucleotides
Atu0489: Atu0489 - methyltransferase, at 476,434 to 477,270
Atu0489
Atu0490: Atu0490 - hypothetical protein, at 477,406 to 478,017
Atu0490
Atu0491: Atu0491 - hypothetical protein, at 478,100 to 478,741
Atu0491
Atu0492: Atu0492 - hypothetical protein, at 478,768 to 479,229
Atu0492
Position (kb) 477
478
479 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 476.410 kb on + strand at 476.604 kb on - strand, within Atu0489 at 476.623 kb on - strand, within Atu0489 at 476.634 kb on - strand, within Atu0489 at 476.647 kb on - strand, within Atu0489 at 476.647 kb on - strand, within Atu0489 at 476.700 kb on + strand, within Atu0489 at 476.767 kb on - strand, within Atu0489 at 476.832 kb on + strand, within Atu0489 at 476.865 kb on + strand, within Atu0489 at 476.943 kb on + strand, within Atu0489 at 477.014 kb on + strand, within Atu0489 at 477.038 kb on + strand, within Atu0489 at 477.048 kb on + strand, within Atu0489 at 477.048 kb on + strand, within Atu0489 at 477.160 kb on - strand, within Atu0489 at 477.300 kb on + strand at 477.307 kb on - strand at 477.318 kb on - strand at 477.421 kb on - strand at 477.436 kb on - strand at 477.496 kb on - strand, within Atu0490 at 477.504 kb on - strand, within Atu0490 at 477.514 kb on + strand, within Atu0490 at 477.544 kb on - strand, within Atu0490 at 477.572 kb on + strand, within Atu0490 at 477.572 kb on + strand, within Atu0490 at 477.608 kb on + strand, within Atu0490 at 477.712 kb on + strand, within Atu0490 at 477.722 kb on + strand, within Atu0490 at 477.742 kb on + strand, within Atu0490 at 477.742 kb on + strand, within Atu0490 at 477.760 kb on + strand, within Atu0490 at 477.886 kb on - strand, within Atu0490 at 477.942 kb on - strand, within Atu0490 at 478.111 kb on - strand at 478.223 kb on - strand, within Atu0491 at 478.262 kb on - strand, within Atu0491 at 478.376 kb on + strand, within Atu0491 at 478.464 kb on + strand, within Atu0491 at 478.482 kb on - strand, within Atu0491 at 478.619 kb on + strand, within Atu0491 at 478.627 kb on - strand, within Atu0491 at 478.648 kb on + strand, within Atu0491 at 478.749 kb on - strand at 478.842 kb on - strand, within Atu0492 at 478.920 kb on - strand, within Atu0492 at 478.950 kb on + strand, within Atu0492 at 478.966 kb on - strand, within Atu0492 at 478.968 kb on - strand, within Atu0492 at 478.997 kb on - strand, within Atu0492
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 476,410 + +1.6 476,604 - Atu0489 0.20 +0.3 476,623 - Atu0489 0.23 +0.1 476,634 - Atu0489 0.24 +0.1 476,647 - Atu0489 0.25 +0.7 476,647 - Atu0489 0.25 -1.3 476,700 + Atu0489 0.32 +0.6 476,767 - Atu0489 0.40 +0.1 476,832 + Atu0489 0.48 -0.6 476,865 + Atu0489 0.51 -0.1 476,943 + Atu0489 0.61 +1.2 477,014 + Atu0489 0.69 -0.2 477,038 + Atu0489 0.72 +0.7 477,048 + Atu0489 0.73 -0.2 477,048 + Atu0489 0.73 +0.2 477,160 - Atu0489 0.87 -0.0 477,300 + +0.5 477,307 - +0.6 477,318 - +0.3 477,421 - -2.1 477,436 - -1.6 477,496 - Atu0490 0.15 -2.9 477,504 - Atu0490 0.16 -1.6 477,514 + Atu0490 0.18 -2.2 477,544 - Atu0490 0.23 +0.7 477,572 + Atu0490 0.27 -0.9 477,572 + Atu0490 0.27 -0.8 477,608 + Atu0490 0.33 -0.5 477,712 + Atu0490 0.50 -1.0 477,722 + Atu0490 0.52 -1.2 477,742 + Atu0490 0.55 -1.4 477,742 + Atu0490 0.55 -0.9 477,760 + Atu0490 0.58 +0.2 477,886 - Atu0490 0.78 -0.8 477,942 - Atu0490 0.88 -2.0 478,111 - +0.3 478,223 - Atu0491 0.19 +1.0 478,262 - Atu0491 0.25 +0.4 478,376 + Atu0491 0.43 +0.2 478,464 + Atu0491 0.57 +0.9 478,482 - Atu0491 0.60 +1.6 478,619 + Atu0491 0.81 +0.2 478,627 - Atu0491 0.82 +0.1 478,648 + Atu0491 0.85 +0.1 478,749 - +0.7 478,842 - Atu0492 0.16 +0.3 478,920 - Atu0492 0.33 +0.5 478,950 + Atu0492 0.39 -0.0 478,966 - Atu0492 0.43 +0.4 478,968 - Atu0492 0.43 -0.6 478,997 - Atu0492 0.50 -0.7
Or see this region's nucleotide sequence