Experiment: A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_321 and PfGW456L13_322 are separated by 150 nucleotides PfGW456L13_322 and PfGW456L13_323 overlap by 4 nucleotides PfGW456L13_323 and PfGW456L13_324 are separated by 70 nucleotides
PfGW456L13_321 - Histidine ammonia-lyase (EC 4.3.1.3), at 375,421 to 376,944
_321
PfGW456L13_322 - Histidine ABC transporter, ATP-binding protein (TC 3.A.1), at 377,095 to 377,925
_322
PfGW456L13_323 - Histidine ABC transporter, permease protein (TC 3.A.1), at 377,922 to 378,773
_323
PfGW456L13_324 - Histidine ABC transporter, histidine-binding protein (TC 3.A.1), at 378,844 to 379,812
_324
Position (kb) 377
378 Strain fitness (log2 ratio) -2
-1
0
1
2 at 376.451 kb on + strand, within PfGW456L13_321 at 376.452 kb on - strand, within PfGW456L13_321 at 376.613 kb on + strand, within PfGW456L13_321 at 376.613 kb on + strand, within PfGW456L13_321 at 376.666 kb on + strand, within PfGW456L13_321 at 376.959 kb on + strand at 376.960 kb on - strand at 376.960 kb on - strand at 376.960 kb on - strand at 376.972 kb on - strand at 377.096 kb on + strand at 377.096 kb on + strand at 377.097 kb on - strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.136 kb on - strand at 377.136 kb on - strand at 377.136 kb on - strand at 377.136 kb on - strand at 377.433 kb on - strand, within PfGW456L13_322 at 377.433 kb on - strand, within PfGW456L13_322 at 377.631 kb on - strand, within PfGW456L13_322 at 377.631 kb on - strand, within PfGW456L13_322 at 377.953 kb on + strand at 377.953 kb on + strand at 377.953 kb on + strand at 377.954 kb on - strand at 377.954 kb on - strand at 377.954 kb on - strand at 377.963 kb on + strand at 377.963 kb on + strand at 377.964 kb on - strand at 377.964 kb on - strand at 378.046 kb on - strand, within PfGW456L13_323 at 378.283 kb on + strand, within PfGW456L13_323 at 378.284 kb on - strand, within PfGW456L13_323 at 378.284 kb on - strand, within PfGW456L13_323 at 378.349 kb on + strand, within PfGW456L13_323 at 378.350 kb on - strand, within PfGW456L13_323 at 378.589 kb on - strand, within PfGW456L13_323 at 378.755 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.845 kb on + strand at 378.845 kb on + strand at 378.845 kb on + strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.04 mg/ml remove 376,451 + PfGW456L13_321 0.68 +0.6 376,452 - PfGW456L13_321 0.68 -2.3 376,613 + PfGW456L13_321 0.78 +0.5 376,613 + PfGW456L13_321 0.78 +1.6 376,666 + PfGW456L13_321 0.82 -0.9 376,959 + -0.1 376,960 - -0.9 376,960 - +0.0 376,960 - -0.4 376,972 - -0.9 377,096 + +0.3 377,096 + +1.5 377,097 - +0.2 377,135 + -0.2 377,135 + -0.5 377,135 + +1.5 377,135 + +1.6 377,135 + +0.0 377,136 - +0.1 377,136 - -0.1 377,136 - +0.3 377,136 - -1.0 377,433 - PfGW456L13_322 0.41 +1.0 377,433 - PfGW456L13_322 0.41 +1.0 377,631 - PfGW456L13_322 0.65 +0.4 377,631 - PfGW456L13_322 0.65 +0.0 377,953 + +0.3 377,953 + -0.8 377,953 + -0.4 377,954 - +0.2 377,954 - -0.7 377,954 - -1.3 377,963 + +0.2 377,963 + +0.9 377,964 - -0.3 377,964 - -0.6 378,046 - PfGW456L13_323 0.15 -0.2 378,283 + PfGW456L13_323 0.42 -1.4 378,284 - PfGW456L13_323 0.42 -0.1 378,284 - PfGW456L13_323 0.42 -0.2 378,349 + PfGW456L13_323 0.50 +0.2 378,350 - PfGW456L13_323 0.50 +0.8 378,589 - PfGW456L13_323 0.78 -0.7 378,755 + +0.3 378,773 + -0.6 378,773 + +0.7 378,773 + -0.6 378,773 + +0.1 378,773 + +0.2 378,774 - -0.2 378,774 - -0.3 378,774 - -1.9 378,774 - -0.8 378,774 - +0.2 378,845 + +0.7 378,845 + +0.7 378,845 + -1.0 378,846 - +0.0 378,846 - +1.8 378,846 - +0.7 378,846 - -0.2 378,846 - +0.5 378,846 - -1.0
Or see this region's nucleotide sequence