Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF747 and GFF748 are separated by 53 nucleotides GFF748 and GFF749 overlap by 4 nucleotides GFF749 and GFF750 are separated by 167 nucleotides GFF750 and GFF751 are separated by 116 nucleotides
GFF747 - Multidrug translocase MdfA, at 105,559 to 106,791
GFF747
GFF748 - Protein ybjI, at 106,845 to 107,660
GFF748
GFF749 - Putative transport protein/putative regulator, at 107,657 to 108,868
GFF749
GFF750 - Transcriptional regulator, TetR family, at 109,036 to 109,656
GFF750
GFF751 - TrkA, Potassium channel-family protein, at 109,773 to 111,326
GFF751
Position (kb)
107
108
109 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 106.663 kb on - strand, within GFF747 at 106.873 kb on - strand at 106.876 kb on - strand at 106.890 kb on - strand at 107.106 kb on + strand, within GFF748 at 107.272 kb on + strand, within GFF748 at 107.515 kb on + strand, within GFF748 at 107.573 kb on - strand, within GFF748 at 107.599 kb on + strand at 107.776 kb on - strand at 107.776 kb on - strand at 107.790 kb on + strand, within GFF749 at 108.037 kb on - strand, within GFF749 at 108.141 kb on - strand, within GFF749 at 108.430 kb on - strand, within GFF749 at 108.587 kb on - strand, within GFF749 at 108.605 kb on + strand, within GFF749 at 108.758 kb on - strand at 108.814 kb on + strand at 108.814 kb on + strand at 108.814 kb on + strand at 108.868 kb on + strand at 108.906 kb on + strand at 108.942 kb on + strand at 108.943 kb on - strand at 108.997 kb on + strand at 109.071 kb on + strand at 109.193 kb on - strand, within GFF750 at 109.369 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.376 kb on + strand, within GFF750 at 109.377 kb on - strand, within GFF750 at 109.377 kb on - strand, within GFF750 at 109.700 kb on - strand at 109.700 kb on - strand at 109.734 kb on + strand at 109.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 106,663 - GFF747 0.90 -0.2 106,873 - -0.7 106,876 - +1.5 106,890 - -0.2 107,106 + GFF748 0.32 -0.2 107,272 + GFF748 0.52 -2.3 107,515 + GFF748 0.82 -0.7 107,573 - GFF748 0.89 -0.4 107,599 + -0.7 107,776 - -0.7 107,776 - -1.0 107,790 + GFF749 0.11 +0.0 108,037 - GFF749 0.31 +2.9 108,141 - GFF749 0.40 +0.4 108,430 - GFF749 0.64 -0.1 108,587 - GFF749 0.77 +3.3 108,605 + GFF749 0.78 +0.7 108,758 - -0.4 108,814 + -1.4 108,814 + -0.5 108,814 + +0.4 108,868 + +0.4 108,906 + -0.4 108,942 + +0.2 108,943 - +0.6 108,997 + +0.5 109,071 + -0.3 109,193 - GFF750 0.25 -0.9 109,369 + GFF750 0.54 +0.3 109,376 + GFF750 0.55 -2.4 109,376 + GFF750 0.55 -0.3 109,376 + GFF750 0.55 +0.3 109,376 + GFF750 0.55 -0.4 109,377 - GFF750 0.55 +0.4 109,377 - GFF750 0.55 +0.3 109,700 - +0.1 109,700 - +0.2 109,734 + +0.7 109,822 - +0.2
Or see this region's nucleotide sequence